Recombinant Mouse Leptin R Protein

COA

级别和纯度:

≥90%(SDS-PAGE)

表达系统: HEK293
Accession #: P48356
蛋白标签: C-His
生物活性: Measured by its ability to inhibit Leptin-dependent proliferation of BaF3 mouse pro‑B cells transfected with human Leptin R. The ED50 for this effect is 2-10 ng/mL in the presence of 1 ng/mL recombinant mouse Leptin.
内毒素水平: <0.1 EU/μg

有货

库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp168605-10μg	10μg	现货
rp168605-50μg	50μg	现货
rp168605-100μg	100μg	现货
rp168605-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: LEPR

Accession#: P48356 (1) Accession#: Q3US58 (1) CD295 (1) Gene ID: 16847 (1) Lepr (3) Leptin R (1)

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基本描述

产品名称	Recombinant Mouse Leptin R Protein
别名	重组小鼠Leptin R蛋白 \| 重组小鼠瘦素受体蛋白
英文别名	Leptin Receptor \| B219 \| CD295 antigen \| CD295 \| DB \| DKFZp686B1731 \| huB219 \| LEPR \| LEP-R \| Leptin R \| LeptinR \| OB R \| OB receptor \| OB-R \| OBR \| obl \| Leprb \| Modb1 \| LEPROT \| OB-RGRP \| diabetes \| obese-like
规格或纯度	ActiBioPure™, 无动物源, Carrier Free, 生物活性, High performance, ≥90%(SDS-PAGE)
产品介绍	Receptor for hormone LEP/leptin (Probable). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity. Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T-cells. Leptin increases Th1 and suppresses Th2 cytokine production. Isoform A May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis. Does not induce phosphorylation of and activate STAT3. Isoform E Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis.
生物活性	Measured by its ability to inhibit Leptin-dependent proliferation of BaF3 mouse pro‑B cells transfected with human Leptin R. The ED50 for this effect is 2-10 ng/mL in the presence of 1 ng/mL recombinant mouse Leptin.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	小鼠(Mouse)
氨基酸	22-839 aa
序列	LNLAYPISPWKFKLFCGPPNTTDDSFLSPAGAPNNASALKGASEAIVEAKFNSSGIYVPELSKTVFHCCFGNEQGQNCSALTDNTEGKTLASVVKASVFRQLGVNWDIECWMKGDLTLFICHMEPLPKNPFKNYDSKVHLLYDLPEVIDDSPLPPLKDSFQTVQCNCSLRGCECHVPVPRAKLNYALLMYLEITSAGVSFQSPLMSLQPMLVVKPDPPLGLHMEVTDDGNLKISWDSQTMAPFPLQYQVKYLENSTIVREAAEIVSATSLLVDSVLPGSSYEVQVRSKRLDGSGVWSDWSSPQVFTTQDVVYFPPKILTSVGSNASFHCIYKNENQIISSKQIVWWRNLAEKIPEIQYSIVSDRVSKVTFSNLKATRPRGKFTYDAVYCCNEQACHHRYAELYVIDVNINISCETDGYLTKMTCRWSPSTIQSLVGSTVQLRYHRRSLYCPDSPSIHPTSEPKNCVLQRDGFYECVFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSNVKAEITVNTGLLKVSWEKPVFPENNLQFQIRYGLSGKEIQWKTHEVFDAKSKSASLLVSDLCAVYVVQVRCRRLDGLGYWSNWSSPAYTLVMDVKVPMRGPEFWRKMDGDVTKKERNVTLLWKPLTKNDSLCSVRRYVVKHRTAHNGTWSEDVGNRTNLTFLWTEPAHTVTVLAVNSLGASLVNFNLTFSWPMSKVSAVESLSAYPLSSSCVILSWTLSPDDYSLLYLVIEWKILNEDDGMKWLRIPSNVKKFYIHDNFIPIEKYQFSLYPVFMEGVGKPKIINGFTKDAIDKQQNDAGHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P48356
来源	重组表达
预测分子量	93 kDa
SDS-PAGE	110 - 130 kDa, under reducing conditions; 110 - 130 kDa, under non-reducing conditions.

储存与运输

储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Mouse Leptin R Protein (rp168605)

Recombinant Mouse Leptin R Protein (rp168605) - SDS-PAGE
2 μg/lane of Recombinant Mouse Leptin R Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 110 - 130 kDa.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0217789	分析证书	rp168605
ZJ25F0217788	分析证书	rp168605
ZJ25F0217787	分析证书	rp168605

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