Recombinant Mouse Ret Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P35546
蛋白标签: C-His
生物活性: Measured by its binding ability in a functional ELISA. Serial dilution of immobilized rmRet (100 µL/well) was tested for binding with the GDNF-GFR alpha -1 complex using 4 ng/mL rhGDNF and 1 µg/mL rrGFR alpha -1/Fc Chimera. The concentration of immobilized Ret that produces 50% of the optimal binding response is found to be approximately 2-5 µg/mL.
内毒素水平: <0.1 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp168533-10μg	10μg	现货
rp168533-50μg	50μg	现货
rp168533-100μg	100μg	现货
rp168533-1mg	1mg	期货

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点击查看蛋白信息: RET

Accession#:P35546 (1) Gene ID:19713 (1) Ret (40)

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基本描述

产品名称	Recombinant Mouse Ret Protein
别名	重组小鼠Ret蛋白
英文别名	Proto-oncogene Tyrosine-protein Kinase Receptor Ret \| CDHF12MTC1 \| CDHR16 \| EC 2.7.10 \| Hirschsprung disease 1 \| Hirschsprung disease) \| MEN2A \| MEN2B \| multiple endocrine neoplasia and medullary thyroid carcinoma 1 \| ret proto-oncogene \| RET transforming
规格或纯度	ActiBioPure™, 生物活性, 无动物源, Carrier Free, ≥95%(SDS-PAGE)
产品介绍	Function Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation in response to glia cell line-derived growth family factors (GDNF, NRTN, ARTN, PSPN and GDF15). In contrast to most receptor tyrosine kinases, RET requires not only its cognate ligands but also coreceptors, for activation. GDNF ligands (GDNF, NRTN, ARTN, PSPN and GDF15) first bind their corresponding GDNFR coreceptors (GFRA1, GFRA2, GFRA3, GFRA4 and GFRAL, respectively), triggering RET autophosphorylation and activation, leading to activation of downstream signaling pathways, including the MAPK- and AKT-signaling pathways. Acts as a dependence receptor via the GDNF-GFRA1 signaling: in the presence of the ligand GDNF in somatotrophs within pituitary, promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Required for the molecular mechanisms orchestration during intestine organogenesis via the ARTN-GFRA3 signaling: involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Also active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Involved in the development of the neural crest. Regulates nociceptor survival and size. Phosphorylates PTK2/FAK1.
生物活性	Measured by its binding ability in a functional ELISA. Serial dilution of immobilized rmRet (100 µL/well) was tested for binding with the GDNF-GFR alpha -1 complex using 4 ng/mL rhGDNF and 1 µg/mL rrGFR alpha -1/Fc Chimera. The concentration of immobilized Ret that produces 50% of the optimal binding response is found to be approximately 2-5 µg/mL.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	小鼠(Mouse)
氨基酸	29-637 aa (F174S)
序列	LYFSRDAYWERLYVDQPAGTPLLYVHALRDAPGEVPSFRLGQHLYGVYRTRLHENDWIRINETTGLLYLNQSLDHSSWEQLSIRNGGFPLLTIFLQVFLGSTAQREGECHWPGCTRVYFSFINDTFPNCSSFKAQDLCIPETAVSSRVRENRPPGTFYHFHMLPVQFLCPNISVKYSLLGGDSLPFRCDPDCLEVSTRWALDRELREKYVLEALCIVAGPGANKETVTLSFPVTVYDEDDSAPTFSGGVGTASAVVEFKRKEGTVVATLQVFDADVVPASGELVRRYTNTLLSGDSWAQQTFRVEHSPIETLVQVNNNSVRATMHNYKLILNRSLSISESRVLQLAVLVNDSDFQGPGAGGILVLHFNVSVLPVTLNLPRAYSFPVNKRARRYAQIGKVCVENCQEFSGVSIQYKLQPSSINCTALGVVTSPEDTSGTLFVNDTEALRRPECTKLQYTVVATDRQTRRQTQASLVVTVEGTSITEEVGCPKSCAVNKRRPECEECGGLGSPTGRCEWRQGDGKGITRNFSTCSPSTRTCPDGHCDAVESRDANICPQDCLRADIVGGHERGERQGIKAGYGICNCFPDEKKCFCEPEDSQGPLCDALCRHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P35546
来源	重组表达
预测分子量	68.4kDa
SDS-PAGE	110-120 kDa under reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。复溶后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Mouse Ret Protein (rp168533)

Recombinant Mouse Ret Protein (rp168533) - SDS-PAGE
2 μg/lane of Recombinant Mouse Ret Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 90 - 110 kDa under reducing conditions and 90 - 110 & 180 - 220 kDa under non-reducing conditions.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0927491	分析证书	rp168533
ZJ25F0927490	分析证书	rp168533
ZJ25F0927489	分析证书	rp168533

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