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Recombinant Human TNF-alpha GMP Protein

  • 表达系统: E. coli
  • Accession #: P01375.1
  • 蛋白标签: No tag
  • 生物活性: Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25-100 pg/mL. The specific activity of recombinant human TNF-α GMP is >4.3 x 10^7 IU/mg, which is calibrated against the human TNF-α WHO International Standard (NIBSC code: 12/154).
  • 内毒素水平: <0.1 EU/μg
有货

库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
rp168404-GMP-100μg
100μg 期货 Stock Image
rp168404-GMP-2mg
2mg 期货 Stock Image

基本描述

产品名称 Recombinant Human TNF-alpha GMP Protein
别名 肿瘤坏死因子重组蛋白 | 重组人 TNF-alpha GMP 蛋白
英文别名 APC1 protein | Cachectin | Cachetin | DIF | TNF | TNF, monocyte-derived | TNFA | TNF-A | TNFalpha | TNF-alpha | TNF-alphacachectin | TNFATNF, macrophage-derived | TNFG1F | TNLG1F | tumor necrosis factor-alpha | tumor necrosis factor | Tumor necrosis facto
规格或纯度 ActiBioPure™, 生物活性, GMP, 无动物源, 无载体, High performance, ≥97%(SDS-PAGE&SEC-HPLC)
生化机理 与 TNFRSF1A/TNFR1 和 TNFRSF1B/TNFBR 结合的细胞因子。它主要由巨噬细胞分泌,能诱导某些肿瘤细胞系的细胞死亡。它是一种强效热原,可通过直接作用或刺激白细胞介素-1分泌引起发热,并与诱导恶病质有关。
生物活性 Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25-100 pg/mL. The specific activity of recombinant human TNF-α GMP is >4.3 x 10^7 IU/mg, which is calibrated against the human TNF-α WHO International Standard (NIBSC code: 12/154).
内毒素水平 <0.1 EU/μg
表达系统 E. coli
种属 人(Human)
氨基酸 77-233 aa
序列 MVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
蛋白标签 No tag
N-末端序列 Met-Val77-Arg-Ser-Ser-Ser-Arg-Thr-Pro-Ser Val77-Arg-Ser-Ser-Ser-Arg-Thr-Pro-Ser-Asp
无载体 Yes
无动物源 Yes
Accession # P01375.1
来源 重组表达
预测分子量 17 kDa
SDS-PAGE 17 kDa, under reducing conditions

储存与运输

物理形态 冻干(Lyophilized)
储存缓冲液 Lyophilized from a 0.2 μm filtered solution in PBS.
复溶 Reconstitute at 0.1-1 mg/mL in PBS.
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 按供货时的温度≤ -20 ℃保存,至少可保存 12 个月。1 个月,复溶后在无菌条件下 2 至 8 ℃ 保存。3 个月,复溶后无菌条件下 ≤ -28 ℃。收货后建议分装。避免冻融循环。
分子类型 蛋白质

质检证书(CoA,COO,BSE/TSE 和分析图谱)

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溶液计算器