Recombinant Mouse MAdCAM-1 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: G5E838
蛋白标签: C-His
生物活性: Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells. When 5 x 104 cells/well are added to mouse MAdCAM-1/Fc Chimera coated plates (10 µg/mL with 100 µL/well), approximately 80-90% will adhere after MnCl2 stimulation for 1 hour incubation at room temperature.
内毒素水平: <0.1 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp168058-10μg	10μg	现货
rp168058-50μg	50μg	现货
rp168058-100μg	100μg	现货
rp168058-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: MADCAM1 点击查看蛋白信息: COG1

Accession#: G5E838 (1) Gene ID: 17123 (2) MADCAM1 (5)

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基本描述

产品名称	Recombinant Mouse MAdCAM-1 Protein
别名	重组小鼠MAdCAM-1蛋白 \| 重组小鼠黏膜地址素细胞黏附分子蛋白
英文别名	Mucosal Addressin Cell Adhesion Molecule 1 \| hMAdCAM-1 \| MACAM1 \| MAdCAM1 \| MAdCAM-1 \| mucosal addressin cell adhesion molecule 1 \| mucosal addressin cell adhesion molecule-1 \| mucosal vascular addressin cell adhesion molecule 1 \| Madcam1
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Purity ≥95% SDS-PAGE. Endotoxin level <0.1 EU/µg Background: Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule family member. In addition to Ig domains, it contains a mucin-like domain and a membrane proximal domain with similarity to IgA. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyer’s patches. It has also been found to be expressed on sinus-lining cells of the spleen. The integrin, alpha4beta7, has been shown to function as the MAdCAM-1 receptor. The Ig domains of MAdCAM-1 have been found to be critical to alpha4beta7binding. The mucin domain has been shown to have activity in L-Selectin binding. MAdCAM-1 expression has been demonstrated to be up-regulated by TNF-alpha and IL-1 beta. MAdCAM-1 appears to play a role in inflammatory bowel disease (IBD) as its expression is highly up-regulated in IBD and most likely serves to recruit alpha4beta7-expressing lymphocytes to the region. In vivo studies involving nonobese diabetic (NOD) mice have also suggested that MAdCAM-1/ alpha4beta7interaction plays a role in diabetes development in this model. Mouse MAdCAM-1 is a 405 amino acid (aa) residue protein with a 21 aa signal sequence, a 344 aa extracellular domain, a 20 aa transmembrane domain and a 20 aa cytoplasmic domain.
生物活性	Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells. When 5 x 104 cells/well are added to mouse MAdCAM-1/Fc Chimera coated plates (10 µg/mL with 100 µL/well), approximately 80-90% will adhere after MnCl2 stimulation for 1 hour incubation at room temperature.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	小鼠(Mouse)
氨基酸	22-365 aa
序列	QSFQVNPPESEVAVAMGTSLQITCSMSCDEGVARVHWRGLDTSLGSVQTLPGSSILSVRGMLSDTGTPVCVGSCGSRSFQHSVKILVYAFPDQLVVSPEFLVPGQDQVVSCTAHNIWPADPNSLSFALLLGEQRLEGAQALEPEQEEEIQEAEGTPLFRMTQRWRLPSLGTPAPPALHCQVTMQLPKLVLTHRKEIPVLQSQTSPKPPNTTSAEPYILTSSSTAEAVSTGLNITTLPSAPPYPKLSPRTLSSEGPCRPKIHQDLEAGWELLCEASCGPGVTVRWTLAPGDLATYHKREAGAQAWLSVLPPGPMVEGWFQCRQDPGGQVTNLYVPGQVTPNSSSTHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	G5E838
来源	重组表达
预测分子量	37.7 kDa
SDS-PAGE	55-60 kDa under reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。复溶后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Mouse MAdCAM-1 Protein (rp168058)

Recombinant Mouse MAdCAM-1 Protein (rp168058) - SDS-PAGE
2 μg/lane of Recombinant Mouse MAdCAM-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 55 - 65 kDa under reducing conditions and 55 - 65 & 120 kDa under non-reducing conditions.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0723982	分析证书	rp168058
ZJ25F0723981	分析证书	rp168058
ZJ25F0723980	分析证书	rp168058

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