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Recombinant Human Doublecortin Protein

Recombinant Human Doublecortin Protein

COA

级别和纯度:

无载体

无叠氮钠

≥90%(SDS-PAGE)

表达系统: E. coli
Accession #: O43602
蛋白标签: N-His
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp156657-10μg	10μg	现货
rp156657-100μg	100μg	现货
rp156657-1mg	1mg	期货

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点击查看蛋白信息: DCX

Accession#:O43602 (1) DCX (1) Doublecortin (1) Gene ID:1641 (1)

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基本描述

产品名称	Recombinant Human Doublecortin Protein
别名	Doublecortin,DCX,DBCN,LISX,Doublin,Lissencephalin-X(Lis-X),Neuronal migration protein doublecortin,重组人Doublecortin蛋白 \| 重组人双皮质素蛋白
英文别名	Doublin \| Lissencephalin-X \| Lis-X
规格或纯度	Carrier Free, Azide Free, ≥90%(SDS-PAGE)
产品介绍	Purity: >90%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description: DCX (doublecortin, N-GST chimera)contains 2 doublecortin domains and belongs to the doublecortin family. It is highly expressed in neuronal cells of fetal brain, but not expressed in other fetal tissues. In the adult, it is highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. DCX is a microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. DCX may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. It may be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration. Defects in DCX are the cause of lissencephaly X-linked type 1 and subcortical band heterotopia X-linked.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	40-150 aa
序列	MGHHHHHHALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNV
纯度	＞90% (SDS-PAGE)
蛋白标签	N-His
无载体	Yes
Accession #	O43602
来源	重组表达
预测分子量	13.2 kDa
SDS-PAGE	13.2 kDa

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, PH7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年，在 2-8°C 下可保存 1 个月。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human Doublecortin Protein (rp156657)

Recombinant Human Doublecortin Protein (rp156657) - SDS-PAGE
3 μg/lane of Recombinant Human Doublecortin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 13.2 kDa.

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ23F0700582	分析证书	rp156657
ZJ23F0700564	分析证书	rp156657

引用文献

1. Kim MH, Cierpicki T, Derewenda U, Krowarsch D, Feng Y, Devedjiev Y, Dauter Z, Walsh CA, Otlewski J, Bushweller JH et al.. (2003) The DCX-domain tandems of doublecortin and doublecortin-like kinase.. Nat Struct Biol, 10 (5): (324-33). [PMID:12692530]
2. des Portes, V V and 13 more authors.. (1998) A novel CNS gene required for neuronal migration and involved in X-linked subcortical laminar heterotopia and lissencephaly syndrome.. Cell, (9): [PMID:9489699]
3. Gleeson, J G JG and 10 more authors.. (1998) Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein.. Cell, (9): [PMID:9489700]
4. des Portes, V V and 15 more authors.. (1998) doublecortin is the major gene causing X-linked subcortical laminar heterotopia (SCLH).. Human molecular genetics, [PMID:9618162]
5. Sossey-Alaoui, K K and 8 more authors.. (1998) Human doublecortin (DCX) and the homologous gene in mouse encode a putative Ca2+-dependent signaling protein which is mutated in human X-linked neuronal migration defects.. Human molecular genetics, [PMID:9668176]
6. Pilz, D T DT and 10 more authors.. (1998) LIS1 and XLIS (DCX) mutations cause most classical lissencephaly, but different patterns of malformation.. Human molecular genetics, [PMID:9817918]
7. Gleeson, J G JG and 24 more authors.. (1999) Characterization of mutations in the gene doublecortin in patients with double cortex syndrome.. Annals of neurology, [PMID:9989615]
8. Kato, M M and 7 more authors.. (1999) A novel mutation of the doublecortin gene in Japanese patients with X-linked lissencephaly and subcortical band heterotopia.. Human genetics, [PMID:10369164]
9. Pilz, D T DT and 7 more authors.. (1999) Subcortical band heterotopia in rare affected males can be caused by missense mutations in DCX (XLIS) or LIS1.. Human molecular genetics, [PMID:10441340]
10. Sakamoto, M M, Ono, J J, Okada, S S, Nakamura, Y Y and Kurahashi, H H.. (2000) Genetic alteration of the DCX gene in Japanese patients with subcortical laminar heterotopia or isolated lissencephaly sequence.. Journal of human genetics, [PMID:10807542]
11. Matsumoto, N N and 11 more authors.. (2001) Mutation analysis of the DCX gene and genotype/phenotype correlation in subcortical band heterotopia.. European journal of human genetics : EJHG, [PMID:11175293]
12. Demelas, L L and 10 more authors.. (2001) Incomplete penetrance with normal MRI in a woman with germline mutation of the DCX gene.. Neurology, (24): [PMID:11468322]
13. Kato, M M and 8 more authors.. (2001) Mutation of the doublecortin gene in male patients with double cortex syndrome: somatic mosaicism detected by hair root analysis.. Annals of neurology, [PMID:11601509]
14. des Portes, V V and 8 more authors.. (2002) So-called 'cryptogenic' partial seizures resulting from a subtle cortical dysgenesis due to a doublecortin gene mutation.. Seizure, [PMID:12027577]
15. D'Agostino, Maria Daniela MD and 31 more authors.. (2002) Subcortical band heterotopia (SBH) in males: clinical, imaging and genetic findings in comparison with females.. Brain : a journal of neurology, [PMID:12390976]
16. Aigner, L L and 14 more authors.. (2003) Somatic mosaicism and variable penetrance in doublecortin-associated migration disorders.. Neurology, (28): [PMID:12552055]
17. Ross, Mark T MT and 281 more authors.. (2005) The DNA sequence of the human X chromosome.. Nature, (17): [PMID:15772651]
18. Slepak, Tatiana I; Salay, Lindsey D; Lemmon, Vance P and Bixby, John L.. (2012) Dyrk kinases regulate phosphorylation of doublecortin, cytoskeletal organization, and neuronal morphology.. Cytoskeleton (Hoboken, N.J.), [PMID:22359282]
19. Homan, Claire C CC and 10 more authors.. (2014) Mutations in USP9X are associated with X-linked intellectual disability and disrupt neuronal cell migration and growth.. American journal of human genetics, (6): [PMID:24607389]
20. Tsai, Meng-Han MH and 9 more authors.. (2016) A novel DCX missense mutation in a family with X-linked lissencephaly and subcortical band heterotopia syndrome inherited from a low-level somatic mosaic mother: Genetic and functional studies.. European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society, [PMID:27292316]

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