Recombinant Human NRG1-beta 1 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: Q02297-11
蛋白标签: No tag
生物活性: Measured in a cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is typically 0.73 ng/mL.
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp156330-10μg	10μg	期货
rp156330-50μg	50μg	期货
rp156330-100μg	100μg	期货
rp156330-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: NRG1

Accession#: Q02297-11 (4) Gene ID:3084 (6) NRG1 (6) NRG1-beta 1 (1)

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基本描述

产品名称	Recombinant Human NRG1-beta 1 Protein
别名	重组人NRG1-beta 1蛋白 \| 重组人类 NRG1-beta 1 蛋白
英文别名	SMDF \| Sensory and motor neuron-derived factor \| ProNRG1 \| Pro-NRG1 \| pro-neuregulin-1, membrane-bound isoform \| OTTHUMP00000230605 \| OTTHUMP00000230603 \| OTTHUMP00000225545 \| OTTHUMP00000225477 \| OTTHUMP00000225422 \| OTTHUMP00000225421 \| OTTHUMP000002254
规格或纯度	ActiBioPure™, 生物活性, Carrier Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Protein Function: Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4 (PubMed:10867024, PubMed:7902537). Binds to ERBB3 (PubMed:20682778). Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:20682778). Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons (By similarity).
生物活性	Measured in a cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is typically 0.73 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	121-191 aa
序列	TSHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCPNEFTGDRCQNYVMASFYKHLGIEFMEAEELYQK
蛋白标签	No tag
无载体	Yes
无动物源	No
Accession #	Q02297-11
来源	重组表达
预测分子量	8.2 kDa
SDS-PAGE	11.1 kDa, under reducing conditions; 11.2 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human NRG1-beta 1 Protein (rp156330)

Recombinant Human NRG1-beta 1 Protein (rp156330) - Protein Bioactivity
Measured in a cell proliferation assay using MCF‑7 human breast cancer cells. The ED₅₀ for this effect is typically 0.73 ng/mL.

SDS-PAGE: Recombinant Human NRG1-beta 1 Protein (rp156330)

Recombinant Human NRG1-beta 1 Protein (rp156330) - SDS-PAGE
3 μg/lane of Recombinant Human NRG1-beta 1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 11.1 kDa under reducing conditions and 11.2 kDa under non-reducing conditions.

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