Recombinant Mouse TNF-alpha Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P06804
蛋白标签: No tag
生物活性: Recombinant Mouse TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 29-116 pg/mL, corresponding to a specific activity of 8.62×10^6-3.45×10^7 units/mg.
内毒素水平: <0.1 EU/μg

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货号 (SKU)	包装规格	是否现货
rp154764-10μg	10μg	现货
rp154764-50μg	50μg	现货
rp154764-100μg	100μg	现货
rp154764-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: TNF

Accession#:P06804 (3) Gene ID:21926 (3) Tnf (22) TNF a (1) TNF-alpha (11) TNF-α (4)

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基本描述

产品名称	Recombinant Mouse TNF-alpha Protein
别名	重组小鼠TNF-alpha蛋白 \| 重组小鼠肿瘤坏死因子α蛋白
英文别名	Tumor Necrosis Factor alpha \| APC1 protein \| Cachectin \| Cachetin \| DIF \| TNF \| TNF, monocyte-derived \| TNFA \| TNF-A \| TNFalpha \| TNF-alphacachectin \| TNF-alpha \| TNFATNF, macrophage-derived \| TNFG1F \| TNFSF1A \| TNFSF2 \| TNF superfamily, member 2 \| tumor
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Purity ≥95% SDS-PAGE. Endotoxin level <0.1 EU/µg Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
生物活性	Recombinant Mouse TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 29-116 pg/mL, corresponding to a specific activity of 8.62×10^6-3.45×10^7 units/mg.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	小鼠(Mouse)
氨基酸	80-235 aa
序列	LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL
纯度	≥95% (SDS-PAGE)
蛋白标签	No tag
无载体	Yes
无动物源	Yes
Accession #	P06804
来源	重组表达
预测分子量	17 kDa
SDS-PAGE	17 kDa under reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。复溶后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Mouse TNF-alpha Protein (rp154764)

Recombinant Mouse TNF-alpha Protein (rp154764) - Protein Bioactivity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (A113142). The ED50 for this effect is 6.6 pg/mL.

SDS-PAGE: Recombinant Mouse TNF-alpha Protein (rp154764)

Recombinant Mouse TNF-alpha Protein (rp154764) - SDS-PAGE
2 μg/lane of Recombinant Mouse TNF-alpha Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 16 - 18 kDa under reducing condition and 16 - 18 & 30 - 32 kDa under non-reducing condition due to glycosylation.

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批号(Lot Number)	证书类型	货号
ZJ25F0926546	分析证书	rp154764
ZJ25F0926545	分析证书	rp154764
ZJ25F0926544	分析证书	rp154764

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Recombinant Mouse TNF-alpha Protein

库存信息

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基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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