Recombinant Mouse IL-10 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P18893
蛋白标签: C-His
生物活性: Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. The ED₅₀ for this effect is 0.8 ng/mL.
内毒素水平: <0.1 EU/μg

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货号 (SKU)	包装规格	是否现货
rp154124-10μg	10μg	现货
rp154124-50μg	50μg	现货
rp154124-100μg	100μg	现货
rp154124-1mg	1mg	期货

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点击查看蛋白信息: IL10

Accession#: P18893 (1) Gene ID: 16153 (1) IL10 (9)

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基本描述

产品名称	Recombinant Mouse IL-10 Protein
别名	重组小鼠白细胞介素10蛋白 \| CSIF \| CSIFMGC126450 \| Cytokine synthesis inhibitory factor \| GVHDS \| IL10 \| IL-10 \| IL10A \| IL-10MGC126451 \| interleukin 10 \| interleukin-10 \| TGIF
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Purity ≥95% (SDS-PAGE) Endotoxin level <0.1 EU/μg Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, interleukin 10 is encoded by the IL10 gene. IL-10 signals through a receptor complex consisting of two IL-10 receptor-1 and two IL-10 receptor-2 proteins. Consequently, the functional receptor consists of four IL-10 receptor molecules. IL-10 binding induces STAT3 signalling via the phosphorylation of the cytoplasmic tails of IL-10 receptor 1 + IL-10 receptor 2 by JAK1 and Tyk2 respectively.
生物活性	Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. The ED₅₀ for this effect is 0.8 ng/mL.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	小鼠(Mouse)
氨基酸	19-178 aa
序列	SRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVEQVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKSHHHHHH
纯度	≥95% (SDS-PAGE)
蛋白标签	C-His
无动物源	Yes
Accession #	P18893
来源	重组表达
预测分子量	19.6 kDa
SDS-PAGE	20-24 kDa, under reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。复溶后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Mouse IL-10 Protein (rp154124)

Recombinant Mouse IL-10 Protein (rp154124) - SDS-PAGE
2 μg/lane of Recombinant Mouse IL-10 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 18 - 22 kDa.

Protein Bioactivity: Recombinant Mouse IL-10 Protein (rp154124)

Recombinant Mouse IL-10 Protein (rp154124) - Protein Bioactivity
Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. The ED50 for this effect is 0.8 ng/mL.

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批号(Lot Number)	证书类型	货号
ZJ25F0724296	分析证书	rp154124
ZJ25F0724295	分析证书	rp154124
ZJ25F0724294	分析证书	rp154124

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