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Recombinant Mouse IL-1 beta Protein

Recombinant Mouse IL-1 beta Protein

级别和纯度:

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: P10749
蛋白标签: C-His
生物活性: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.The ED₅₀ for this effect is typically 10-50 pg/mL.
内毒素水平: <1.0 EU/μg

有货

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库存信息

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货号 (SKU)	包装规格	是否现货
rp154118-10μg	10μg	现货
rp154118-50μg	50μg	现货
rp154118-100μg	100μg	现货
rp154118-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: IL1B

Accession#:P10749 (1) Gene ID:16176 (1) IL-1 beta (5)

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基本描述

产品名称	Recombinant Mouse IL-1 beta Protein
别名	重组小鼠IL-1 β蛋白 \| 重组小鼠IL-1 beta蛋白 \| 重组小鼠 IL-1β 蛋白
英文别名	Catabolin \| H1 \| IFN beta inducing factor \| IL 1 \| IL 1 beta \| IL-1 beta \| IL1 \| IL1 BETA \| IL1B \| Il-1b \| IL1B_HUMAN \| IL1F2 \| Interleukin 1 beta \| Interleukin 1 beta precursor \| interleukin 1, beta \| Interleukin-1 beta \| OAF \| Osteoclast activating fact
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Background： IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65%-78% identity with canine, equine, feline, human, porcine, and rhesus IL-1 beta. Post-translational modifications： Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
生物活性	Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.The ED₅₀ for this effect is typically 10-50 pg/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	小鼠(Mouse)
氨基酸	118-269 aa
序列	MVPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSSHHHHHH
纯度	＞95%(SDS-PAGE)
蛋白标签	C-His
N-末端序列	Met
蛋白长度	Full length protein
无载体	Yes
无动物源	Yes
偶联	Unconjugated
Accession #	P10749
来源	重组表达
预测分子量	18 kDa
SDS-PAGE	16.1 kDa, under reducing conditions; 16.1 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	PBS, pH 7.4, 5%Trehalose, 5%Mannitol
复溶	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is recommended to reconstitute to a concentration of 0.1-1mg/mL.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	建议在无菌条件下于 -20℃~ -80℃ 保存 1 年以上。避免反复冻融循环
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Mouse IL-1 beta Protein (rp154118)

Recombinant Mouse IL-1 beta Protein (rp154118)-SDS-PAGE
3μg/lane of Recombinant Mouse IL-1 beta was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 16.1 kDa.

Protein Bioactivity: Recombinant Mouse IL-1 beta Protein (rp154118)

Recombinant Mouse IL-1 beta Protein (rp154118) - Protein Bioactivity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 10 - 50 pg/mL.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到11个结果

批号(Lot Number)	证书类型	货号
ZJ24F1214755	分析证书	rp154118
ZJ24F1214756	分析证书	rp154118
ZJ24F1214832	分析证书	rp154118
ZJ24F1214833	分析证书	rp154118
ZJ23F0500160	分析证书	rp154118
ZJ23F0500163	分析证书	rp154118
ZJ23F0900976	分析证书	rp154118
ZJ23F0500161	分析证书	rp154118
ZJ24F1215121	分析证书	rp154118
ZJ24F1215122	分析证书	rp154118
ZJ24F1215123	分析证书	rp154118

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