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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| rp154082-10μg |
10μg |
现货 ![]() |
| |
| rp154082-50μg |
50μg |
现货 ![]() |
| |
| rp154082-100μg |
100μg |
现货 ![]() |
| |
| rp154082-1mg |
1mg |
期货 ![]() |
|
| 产品名称 | Recombinant Mouse ICAM-1/CD54 Protein |
|---|---|
| 别名 | 细胞间粘附分子1(ICAM1)重组蛋白 | 重组小鼠ICAM-1/CD54蛋白 |
| 英文别名 | CD54 antigen | CD54 | cell surface glycoprotein P3.58 | human rhinovirus receptor | ICAM1 | ICAM-1 | intercellular adhesion molecule 1 (CD54), human rhinovirus receptor | intercellular adhesion molecule 1 | BB2 | Major group rhinovirus receptor | P3.58 | |
| 规格或纯度 | ActiBioPure™, Bioactive, Animal Free, Carrier Free, High performance, ≥95%(SDS-PAGE) |
| 产品介绍 |
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. Post-translational Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
| 生物活性 | Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated Jurkat cells. When 5 x 104 cells/well are added to Recombinant Mouse ICAM-1/CD54 Protein (rp154082) coated plates (10 µg/mL with 100 µL/well), approximately 65 - 95% will adhere after 1 hour incubation at 37℃. |
| 内毒素水平 | <0.1 EU/μg |
| 表达系统 | HEK293 |
| 种属 | 小鼠(Mouse) |
| 氨基酸 | 28-485 aa |
| 序列 | QVSIHPREAFLPQGGSVQVNCSSSCKEDLSLGLETQWLKDELESGPNWKLFELSEIGEDSSPLCFENCGTVQSSASATITVYSFPESVELRPLPAWQQVGKDLTLRCHVDGGAPRTQLSAVLLRGEEILSRQPVGGHPKDPKEITFTVLASRGDHGANFSCRTELDLRPQGLALFSNVSEARSLRTFDLPATIPKLDTPDLLEVGTQQKLFCSLEGLFPASEARIYLELGGQMPTQESTNSSDSVSATALVEVTEEFDRTLPLRCVLELADQILETQRTLTVYNFSAPVLTLSQLEVSEGSQVTVKCEAHSGSKVVLLSGVEPRPPTPQVQFTLNASSEDHKRSFFCSAALEVAGKFLFKNQTLELHVLYGPRLDETDCLGNWTWQEGSQQTLKCQAWGNPSPKMTCRRKADGALLPIGVVKSVKQEMNGTYVCHAFSSHGNVTRNVYLTVLYHSQNNHHHHHH |
| 蛋白标签 | C-His |
| 无载体 | Yes |
| 无动物源 | Yes |
| Accession # | P13597 |
| 来源 | 重组表达 |
| 预测分子量 | 51 kDa |
| SDS-PAGE | 70 - 100 kDa, under reducing conditions; 65 - 95 kDa, under non-reducing conditions. |
| 物理形态 | 冻干(Lyophilized) |
|---|---|
| 储存缓冲液 | Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4 |
| 复溶 | Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml. |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 蛋白质 |
Recombinant Mouse ICAM-1/CD54 Protein (rp154082) - Protein Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated Jurkat cells. When 5 x 104 cells/well are added to Recombinant Mouse ICAM-1/CD54 Protein (rp154082) coated plates (10 µg/mL with 100 µL/well), approximately 65 - 95% will adhere after 1 hour incubation at 37℃.
Recombinant Mouse ICAM-1/CD54 Protein (rp154082) - SDS-PAGE
3 μg/lane of Recombinant Mouse ICAM-1/CD54 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 70 - 100 kDa under reducing condition and 65 - 95 kDa under non-reducing condition.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp154082 | |
| 分析证书 | rp154082 | |
| 分析证书 | rp154082 | |
| 分析证书 | rp154082 | |
| 分析证书 | rp154082 | |
| 分析证书 | rp154082 |