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Recombinant Mouse ICAM-1/CD54 Protein

  • 表达系统: HEK293
  • Accession #: P13597
  • 蛋白标签: C-His
  • 生物活性: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated Jurkat cells. When 5 x 104 cells/well are added to Recombinant Mouse ICAM-1/CD54 Protein (rp154082) coated plates (10 µg/mL with 100 µL/well), approximately 65 - 95% will adhere after 1 hour incubation at 37℃.
  • 内毒素水平: <0.1 EU/μg
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库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
rp154082-10μg
10μg 现货 Stock Image
rp154082-50μg
50μg 现货 Stock Image
rp154082-100μg
100μg 现货 Stock Image
rp154082-1mg
1mg 期货 Stock Image

基本描述

产品名称 Recombinant Mouse ICAM-1/CD54 Protein
别名 细胞间粘附分子1(ICAM1)重组蛋白 | 重组小鼠ICAM-1/CD54蛋白
英文别名 CD54 antigen | CD54 | cell surface glycoprotein P3.58 | human rhinovirus receptor | ICAM1 | ICAM-1 | intercellular adhesion molecule 1 (CD54), human rhinovirus receptor | intercellular adhesion molecule 1 | BB2 | Major group rhinovirus receptor | P3.58 |
规格或纯度 ActiBioPure™, Bioactive, Animal Free, Carrier Free, High performance, ≥95%(SDS-PAGE)
产品介绍


ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Post-translational

Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

生物活性 Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated Jurkat cells. When 5 x 104 cells/well are added to Recombinant Mouse ICAM-1/CD54 Protein (rp154082) coated plates (10 µg/mL with 100 µL/well), approximately 65 - 95% will adhere after 1 hour incubation at 37℃.
内毒素水平 <0.1 EU/μg
表达系统 HEK293
种属 小鼠(Mouse)
氨基酸 28-485 aa
序列 QVSIHPREAFLPQGGSVQVNCSSSCKEDLSLGLETQWLKDELESGPNWKLFELSEIGEDSSPLCFENCGTVQSSASATITVYSFPESVELRPLPAWQQVGKDLTLRCHVDGGAPRTQLSAVLLRGEEILSRQPVGGHPKDPKEITFTVLASRGDHGANFSCRTELDLRPQGLALFSNVSEARSLRTFDLPATIPKLDTPDLLEVGTQQKLFCSLEGLFPASEARIYLELGGQMPTQESTNSSDSVSATALVEVTEEFDRTLPLRCVLELADQILETQRTLTVYNFSAPVLTLSQLEVSEGSQVTVKCEAHSGSKVVLLSGVEPRPPTPQVQFTLNASSEDHKRSFFCSAALEVAGKFLFKNQTLELHVLYGPRLDETDCLGNWTWQEGSQQTLKCQAWGNPSPKMTCRRKADGALLPIGVVKSVKQEMNGTYVCHAFSSHGNVTRNVYLTVLYHSQNNHHHHHH
蛋白标签 C-His
无载体 Yes
无动物源 Yes
Accession # P13597
来源 重组表达
预测分子量 51 kDa
SDS-PAGE 70 - 100 kDa, under reducing conditions; 65 - 95 kDa, under non-reducing conditions.

储存与运输

物理形态 冻干(Lyophilized)
储存缓冲液 Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶 Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型 蛋白质

图片

Recombinant Mouse ICAM-1/CD54 Protein (rp154082) - Protein Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated Jurkat cells. When 5 x 104 cells/well are added to Recombinant Mouse ICAM-1/CD54 Protein (rp154082) coated plates (10 µg/mL with 100 µL/well), approximately 65 - 95% will adhere after 1 hour incubation at 37℃.

Recombinant Mouse ICAM-1/CD54 Protein (rp154082) - SDS-PAGE
3 μg/lane of Recombinant Mouse ICAM-1/CD54 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 70 - 100 kDa under reducing condition and 65 - 95 kDa under non-reducing condition.

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找到6个结果

批号(Lot Number) 证书类型 货号
ZJ24F1214984 分析证书 rp154082
ZJ24F1011557 分析证书 rp154082
ZJ24F1011573 分析证书 rp154082
ZJ24F1011574 分析证书 rp154082
ZJ24F1214982 分析证书 rp154082
ZJ24F1214983 分析证书 rp154082

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