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Recombinant Human Serpin F1/PEDF Protein

Recombinant Human Serpin F1/PEDF Protein

级别和纯度:

≥97%(SDS-PAGE&HPLC)

表达系统: E. coli
Accession #: P36955
蛋白标签: No tag
生物活性: Fully biologically active when compared to standard. The ED50 as determined by its ability to enhance the adhesion of human Saos2 cells to bovine Collagen I coated plate is less than 2 ng/ml.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp150051-10μg	10μg	现货
rp150051-20μg	20μg	期货
rp150051-50μg	50μg	现货
rp150051-100μg	100μg	现货
rp150051-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: SERPINF1

Accession#:P36955 (2) Gene ID:5176 (2) PEDF (2) SERPINF1 (2)

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基本描述

产品名称	Recombinant Human Serpin F1/PEDF Protein
别名	重组人色素上皮衍生因子蛋白 \| 重组人丝氨酸蛋白酶抑制剂 F1/PEDF 蛋白
英文别名	Cell proliferation-inducing gene 35 protein \| EPC-1 \| EPC-1PIG35 \| PEDF \| PEDFpigment epithelium-derived factor \| pigment epithelium derived factor), member 1 \| proliferation-inducing protein 35 \| serine (or cysteine) proteinase inhibitor, clade F (alpha-
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE&HPLC)
产品介绍	Purity ＞97% by SDS-PAGE and HPLC analyses. Function Pigment epithelium-derived factor (PEDF) is encoded by the SERPINF1 gene in humans and found in verebrates. It is a secreted phosphoglycoprotein that belongs to the clade F subfamily, serpin superfamily of proteinase inhibitors. The PEDF is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is synthesized as a 418 a.a. about 50kDa precursor that contains a 19 a.a. signal sequence and a 399 a.a. mature region that shows a pyroglutamate at Gln20. Like other serpins, it contains three β-sheets, 810 α-helices, and a C-terminal RCL (reactive center loop). Unlike other serpins with Ser protease inhibiting activity. PEDF has functions of inducing extensive neuronal differentiation in retinoblastoma cells, inhibiting of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. PEDF is researched as a therapeutic candidate for treatment of such conditions as choroidal neovascularization, heart disease, and cancer.
生物活性	Fully biologically active when compared to standard. The ED50 as determined by its ability to enhance the adhesion of human Saos2 cells to bovine Collagen I coated plate is less than 2 ng/ml.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	20-418 aa
序列	QNPASPPEEG SPDPDSTGAL VEEEDPFFKV PVNKLAAAVS NFGYDLYRVR SSTSPTTNVL LSPLSVATAL SALSLGAEQR TESIIHRALY YDLISSPDIH GTYKELLDTV TAPQKNLKSA SRIVFEKKLR IKSSFVAPLE KSYGTRPRVL TGNPRLDLQE INNWVQAQMK GKLARSTKEI PDEISILLLG VAHFKGQWVT KFDSRKTSLE DFYLDEERTV RVPMMSDPKA VLRYGLDSDL SCKIAQLPLT GSMSIIFFLP LKVTQNLTLI EESLTSEFIH DIDRELKTVQ AVLTVPKLKL SYEGEVTKSL QEMKLQSLFD SPDFSKITGK PIKLTQVEHR AGFEWNEDGA GTTPSPGLQP AHLTFPLDYH LNQPFIFVLR DTDTGALLFI GKILDPRGP
纯度	＞97%(SDS-PAGE, HPLC)
蛋白标签	No tag
蛋白长度	Full length protein
无载体	Yes
无动物源	Yes
Accession #	P36955
来源	重组表达
预测分子量	44.4kDa

储存与运输

应用	Active, SDS-PAGE
物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	使用手动除霜冰柜，避免反复冻融循环。自收到之日起 12 个月，-20 至 -70 ℃。1 个月，复溶后无菌条件下 2 至 8 ℃。3 个月，复溶后无菌条件下 -20 至 -70 ℃。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human Serpin F1/PEDF (rp150051)

Recombinant Human Serpin F1/PEDF (rp150051)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to enhance the adhesion of human Saos2 cells to bovine Collagen I coated plate is less than 2 ng/mL, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.

Recombinant Human Serpin F1/PEDF Protein

Recombinant Human Serpin F1/PEDF (rp150051) -SDS-PAGE
Recombinant Human Serpin F1/PEDF Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a single band at 44.4 kDa.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F0600476	分析证书	rp150051
ZJ23F0600477	分析证书	rp150051
ZJ23F0600478	分析证书	rp150051

引用文献

1. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940]
2. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]
3. Becerra, S P SP, Sagasti, A A, Spinella, P P and Notario, V V.. (1995) Pigment epithelium-derived factor behaves like a noninhibitory serpin. Neurotrophic activity does not require the serpin reactive loop.. The Journal of biological chemistry, (27): [PMID:7592790]
4. Becerra, S P SP and 6 more authors.. (1993) Overexpression of fetal human pigment epithelium-derived factor in Escherichia coli. A functionally active neurotrophic factor.. The Journal of biological chemistry, (5): [PMID:8226833]
5. Steele, F R FR, Chader, G J GJ, Johnson, L V LV and Tombran-Tink, J J.. (1993) Pigment epithelium-derived factor: neurotrophic activity and identification as a member of the serine protease inhibitor gene family.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:8434014]
6. Pignolo, R J RJ, Cristofalo, V J VJ and Rotenberg, M O MO.. (1993) Senescent WI-38 cells fail to express EPC-1, a gene induced in young cells upon entry into the G0 state.. The Journal of biological chemistry, (25): [PMID:8473338]
7. Tombran-Tink, J J and 6 more authors.. (1996) Organization, evolutionary conservation, expression and unusual Alu density of the human gene for pigment epithelium-derived factor, a unique neurotrophic serpin.. Molecular vision, (4): [PMID:9238088]
8. Koenekoop, R R and 6 more authors.. (1999) Four polymorphic variations in the PEDF gene identified during the mutation screening of patients with Leber congenital amaurosis.. Molecular vision, (2): [PMID:10398730]
9. Simonovic, M M, Gettins, P G PG and Volz, K K.. (2001) Crystal structure of human PEDF, a potent anti-angiogenic and neurite growth-promoting factor.. Proceedings of the National Academy of Sciences of the United States of America, (25): [PMID:11562499]
10. Petersen, Steen V SV, Valnickova, Zuzana Z and Enghild, Jan J JJ.. (2003) Pigment-epithelium-derived factor (PEDF) occurs at a physiologically relevant concentration in human blood: purification and characterization.. The Biochemical journal, (15): [PMID:12737624]
11. Maik-Rachline, Galia G, Shaltiel, Shmuel S and Seger, Rony R.. (2005) Extracellular phosphorylation converts pigment epithelium-derived factor from a neurotrophic to an antiangiogenic factor.. Blood, (15): [PMID:15374885]
12. Liu, Tao T and 6 more authors.. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952]
13. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196]
14. Notari, Luigi L and 12 more authors.. (2006) Identification of a lipase-linked cell membrane receptor for pigment epithelium-derived factor.. The Journal of biological chemistry, (8): [PMID:17032652]
15. Chi, An A and 16 more authors.. (2006) Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.. Journal of proteome research, [PMID:17081065]
16. Becker, Jutta J and 16 more authors.. (2011) Exome sequencing identifies truncating mutations in human SERPINF1 in autosomal-recessive osteogenesis imperfecta.. American journal of human genetics, (11): [PMID:21353196]
17. Oshikawa, Mio M and 8 more authors.. (2011) Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.. Investigative ophthalmology & visual science, (22): [PMID:21697133]
18. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912]

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Recombinant Human Serpin F1/PEDF Protein

库存信息

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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引用文献

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