Recombinant Human PTH1R/PTHR1 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: Q03431
蛋白标签: C-His
生物活性: When Recombinant Human PTH1R/PTHR1 is used at 2 μg/mL, the concentration of biotinylated human PTHrP that produces 50% of the optimal binding response is found to be approximately 0.03‑0.12 μg/mL.
内毒素水平: <0.1 EU/μg

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货号 (SKU)	包装规格	是否现货
rp149790-10μg	10μg	现货
rp149790-50μg	50μg	现货
rp149790-100μg	100μg	现货
rp149790-1mg	1mg	期货

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点击查看蛋白信息: PTH1R

Accession#: Q03431 (2) Gene ID: 5745 (2) Parathyroid Hormone Receptor 1 (2) PTH1R (2) PTHR1 (1) TH1R (1)

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基本描述

产品名称	Recombinant Human PTH1R/PTHR1 Protein
别名	重组人PTH1R/PTHR1蛋白 \| 重组人甲状旁腺素受体1蛋白
英文别名	Parathyroid hormone 1 receptor \| PTH/PTHr receptor \| PTH/PTHrP type I receptor \| PTH1 receptor \| Parathyroid Hormone Receptor 1 \| MGC138426 \| parathyroid hormone 1 receptor \| MGC138452 \| parathyroid hormone/parathyroid hormone-related peptide receptor \| p
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity ≥95% SDS-PAGE. Endotoxin level <0.1 EU/µg Function Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
生物活性	When Recombinant Human PTH1R/PTHR1 is used at 2 μg/mL, the concentration of biotinylated human PTHrP that produces 50% of the optimal binding response is found to be approximately 0.03‑0.12 μg/mL.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	27-188 aa
序列	DADDVMTKEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKPRKDKASGKLYPESEEDKEAPTGSRYRGRPCLPEWDHILCWPLGAPGEVVAVPCPDYIYDFNHKGHAYRRCDRNGSWELVPGHNRTWANYSECVKFLTNETREREVFDRLGHHHHHH
纯度	≥95% (SDS-PAGE)
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	Q03431
来源	重组表达
预测分子量	20 kDa
SDS-PAGE	25 - 40 kDa, under reducing conditions; 25 - 40 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human PTH1R/PTHR1 Protein (rp149790)

Recombinant Human PTH1R/PTHR1 Protein (rp149790) - SDS-PAGE
3 μg/lane of Recombinant Human PTH1R/PTHR1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 25 - 40 kDa.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F1214940	分析证书	rp149790
ZJ24F1214941	分析证书	rp149790
ZJ24F1214942	分析证书	rp149790

引用文献

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12. Rozeman LB, Sangiorgi L, Briaire-de Bruijn IH, Mainil-Varlet P, Bertoni F, Cleton-Jansen AM, Hogendoorn PC, Bovée JV. (2004) Enchondromatosis (Ollier disease, Maffucci syndrome) is not caused by the PTHR1 mutation p.R150C.. Hum Mutat, 24 (6): (466-73). [PMID:15523647]
13. Hoare SR, Usdin TB. (2000) Tuberoinfundibular peptide (7-39) [TIP(7-39)], a novel, selective, high-affinity antagonist for the parathyroid hormone-1 receptor with no detectable agonist activity.. J Pharmacol Exp Ther, 295 (2): (761-70). [PMID:11046116]
14. Hoare SR, Clark JA, Usdin TB. (2000) Molecular determinants of tuberoinfundibular peptide of 39 residues (TIP39) selectivity for the parathyroid hormone-2 (PTH2) receptor. N-terminal truncation of TIP39 reverses PTH2 receptor/PTH1 receptor binding selectivity.. J Biol Chem, 275 (35): (27274-83). [PMID:10854439]
15. Decker E, Stellzig-Eisenhauer A, Fiebig BS, Rau C, Kress W, Saar K, Rüschendorf F, Hubner N, Grimm T, Weber BH. (2008) PTHR1 loss-of-function mutations in familial, nonsyndromic primary failure of tooth eruption.. Am J Hum Genet, 83 (6): (781-6). [PMID:19061984]
16. Okazaki M, Ferrandon S, Vilardaga JP, Bouxsein ML, Potts JT, Gardella TJ. (2008) Prolonged signaling at the parathyroid hormone receptor by peptide ligands targeted to a specific receptor conformation.. Proc Natl Acad Sci USA, 105 (43): (16525-30). [PMID:18946036]
17. Carter PH, Liu RQ, Foster WR, Tamasi JA, Tebben AJ, Favata M, Staal A, Cvijic ME, French MH, Dell V et al.. (2007) Discovery of a small molecule antagonist of the parathyroid hormone receptor by using an N-terminal parathyroid hormone peptide probe.. Proc Natl Acad Sci USA, 104 (16): (6846-51). [PMID:17428923]
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