Recombinant Human Nectin-3 Protein

COA

级别和纯度:

≥98%(SDS-PAGE)

表达系统: HEK293
Accession #: Q9NQS3
蛋白标签: C-His
生物活性: Recombinant Human Nectin-3 Protein is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. Human PVRL1/NECTIN1 hFc has been identified as an interactor of Nectin-3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human Nectin-3 and recombinant PVRL1/NECTIN1 hFc.
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp149161-10μg	10μg	现货
rp149161-50μg	50μg	现货
rp149161-100μg	100μg	现货
rp149161-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: NECTIN3

Accession#:Q9NQS3 (2) Gene ID:25945 (2) NECTIN3 (2)

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基本描述

产品名称	Recombinant Human Nectin-3 Protein
别名	重组人类 Nectin-3 蛋白
英文别名	CDw113 \| Nectin cell adhesion molecule 3 \| Poliovirus receptor-related protein 3
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥98%(SDS-PAGE)
产品介绍	Purity >98% SDS-PAGE. Function Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.
生物活性	Recombinant Human Nectin-3 Protein is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. Human PVRL1/NECTIN1 hFc has been identified as an interactor of Nectin-3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human Nectin-3 and recombinant PVRL1/NECTIN1 hFc.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	58-400 aa
序列	GPIIVEPHVTAVWGKNVSLKCLIEVNETITQISWEKIHGKSSQTVAVHHPQYGFSVQGEYQGRVLFKNYSLNDATITLHNIGFSDSGKYICKAVTFPLGNAQSSTTVTVLVEPTVSLIKGPDSLIDGGNETVAAICIAATGKPVAHIDWEGDLGEMESTTTSFPNETATIISQYKLFPTRFARGRRITCVVKHPALEKDIRYSFILDIQYAPEVSVTGYDGNWFVGRKGVNLKCNADANPPPFKSVWSRLDGQWPDGLLASDNTLHFVHPLTFNYSGVYICKVTNSLGQRSDQKVIYISDPPTTTTLQPTIQWHPSTADIEDLATEPKKLPFPLSTLATIKDDAHHHHHHHHHH
纯度	＞98%(SDS-PAGE)
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	Q9NQS3
来源	重组表达
预测分子量	39.3 kDa
SDS-PAGE	71.1 kDa, under reducing conditions; 68.0 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from sterile PBS, pH7.4, 5% Trehalose, 5% Mannitol.
复溶	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.59mg/mL.
储存温度	-80℃储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	样品自收到之日起在-70℃条件下可稳定保存 12 个月。收到样品后在无菌条件下保存，温度为 -20℃ 至 -80℃。建议将蛋白质分装成较小的量，以达到最佳储存效果。避免反复冻融循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human Nectin-3 Protein (rp149161)

Recombinant Human Nectin-3 Protein (rp149161) - Protein Bioactivity
Recombinant Human Nectin-3 Protein is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. Human PVRL1/NECTIN1 hFc has been identified as an interactor of Nectin-3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human Nectin-3 and recombinant PVRL1/NECTIN1 hFc.

SDS-PAGE: Recombinant Human Nectin-3 Protein (rp149161)

Recombinant Human Nectin-3 Protein (rp149161) - SDS-PAGE
2.5μg/lane of Recombinant Human Nectin-3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 71.1 kDa under reducing conditions and 68.0 kDa under non-reducing conditions.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F0700588	分析证书	rp149161
ZJ23F0700587	分析证书	rp149161
ZJ23F0700586	分析证书	rp149161

引用文献

1. Yu X, Harden K, Gonzalez LC, Francesco M, Chiang E, Irving B, Tom I, Ivelja S, Refino CJ, Clark H et al.. (2009) The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.. Nat Immunol, 10 (1): (48-57). [PMID:19011627]
2. Reymond, N N and 6 more authors.. (2000) Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin.. Gene, (19): [PMID:11024295]
3. Mueller, Steffen S and Wimmer, Eckard E.. (2003) Recruitment of nectin-3 to cell-cell junctions through trans-heterophilic interaction with CD155, a vitronectin and poliovirus receptor that localizes to alpha(v)beta3 integrin-containing membrane microdomains.. The Journal of biological chemistry, (15): [PMID:12759359]
4. Fujito, Tsutomu T and 7 more authors.. (2005) Inhibition of cell movement and proliferation by cell-cell contact-induced interaction of Necl-5 with nectin-3.. The Journal of cell biology, (10): [PMID:16216929]
5. Muzny, Donna M DM and 113 more authors.. (2006) The DNA sequence, annotation and analysis of human chromosome 3.. Nature, (27): [PMID:16641997]
6. Harrison, Oliver J OJ and 10 more authors.. (2012) Nectin ectodomain structures reveal a canonical adhesive interface.. Nature structural & molecular biology, [PMID:22902367]
7. LaFrance, Michelle E ME and 5 more authors.. (2015) Identification of an epithelial cell receptor responsible for Clostridium difficile TcdB-induced cytotoxicity.. Proceedings of the National Academy of Sciences of the United States of America, (2): [PMID:26038560]

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