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Recombinant Human CCL8/MCP-2 Protein

COA COA
  • 表达系统: E. coli
  • Accession #: P80075
  • 蛋白标签: No tag
  • 生物活性: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.
  • 内毒素水平: <1.0 EU/μg
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货号 (SKU) 包装规格 是否现货 价格 数量
rp148607-10μg
 10μg 现货 Stock Image
rp148607-20μg
 20μg 期货 Stock Image
rp148607-50μg
 50μg 现货 Stock Image
rp148607-100μg
 100μg 现货 Stock Image
rp148607-1mg
 1mg 期货 Stock Image
大包装询价 收藏夹 比较  SDS下载  DATASHEET  质量标准
查看相关系列
点击查看蛋白信息: CCL8
Accession#:P80075 (2) CCL8 (3) Gene ID:6355 (2) MCP2 (2)
main product photo

基本描述

产品名称 Recombinant Human CCL8/MCP-2 Protein
别名 重组人 CCL8/MCP-2 蛋白 | 重组人 CCL8/MCP-2 蛋白
英文别名 C-C motif chemokine 8 | CCL8 | Chemokine (C-C motif) ligand 8 | HC14 | MCP2 | MCP-2 | Member 8 (monocyte chemotactic protein 2) | Monocyte chemoattractant protein 2 | SCYA10 | Small-inducible cytokine A8 | HC14 | MCP-2 | Monocyte chemoattractant protein 2
规格或纯度 ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥96%(SDS-PAGE&HPLC)
产品介绍


Purity Human CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is belonging to the CC chemokine family. It is encoded by the gene CCL8. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic function for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells.

Purity
>96% by SDS-PAGE and HPLC analyses.

Additional sequence information
Full-length mature C-C motif chemokine 8 chain lacking the signal peptide.

Function
Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.

Post-translational
N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.

MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.
Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
生物活性 Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.
内毒素水平 <1.0 EU/μg
表达系统 E. coli
种属 人(Human)
氨基酸 24-99 aa
序列 QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP
纯度 ＞96%( SDS-PAGE and HPLC)
蛋白标签 No tag
无载体 Yes
无动物源 Yes
Accession # P80075
来源 重组表达
预测分子量 8.9 kDa
SDS-PAGE 8.9 kDa

储存与运输

应用 Cell Culture,SDS-PAGE,Active standard
物理形态 冻干(Lyophilized)
储存缓冲液 Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
复溶 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 交付后等分，储存于 -20°C，避免冷冻/解冻循环。冻干，无添加剂。本品为活性蛋白质，可能会引起体内生物反应，请谨慎处理。
分子类型 蛋白质

图片

Recombinant Human CCL8/MCP-2 Protein (rp148607)-Protein Bioactivity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/mL.

Recombinant Human CCL8/MCP-2 Protein (rp148607)-SDS-PAGE
Recombinant Human CCL8/MCP-2 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, Showing a single band at 7.8 kDa.

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ23F0600400 分析证书 rp148607
ZJ23F0600401 分析证书 rp148607
ZJ23F0600399 分析证书 rp148607

可替换产品

  1. Recombinant Human CCL8/MCP-2 Protein
    Recombinant Human CCL8/MCP-2 Protein

    ¥719.90

引用文献

1. Proost P, Struyf S, Couvreur M, Lenaerts JP, Conings R, Menten P, Verhaert P, Wuyts A, Van Damme J.  (1998)  Posttranslational modifications affect the activity of the human monocyte chemotactic proteins MCP-1 and MCP-2: identification of MCP-2(6-76) as a natural chemokine inhibitor..  J Immunol,  160  (8): (4034-41).  [PMID:9558113]
2. Blaszczyk J, Coillie EV, Proost P, Damme JV, Opdenakker G, Bujacz GD, Wang JM, Ji X.  (2000)  Complete crystal structure of monocyte chemotactic protein-2, a CC chemokine that interacts with multiple receptors..  Biochemistry,  39  (46): (14075-81).  [PMID:11087354]
3. Van Damme, J J, Proost, P P, Lenaerts, J P JP and Opdenakker, G G..  (1992)  Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family..  The Journal of experimental medicine,  (1): [PMID:1613466]
4. Chang, H C HC, Hsu, F F, Freeman, G J GJ, Griffin, J D JD and Reinherz, E L EL..  (1989)  Cloning and expression of a gamma-interferon-inducible gene in monocytes: a new member of a cytokine gene family..  International immunology,  [PMID:2518726]
5. Kim, K S KS, Rajarathnam, K K, Clark-Lewis, I I and Sykes, B D BD..  (1996)  Structural characterization of a monomeric chemokine: monocyte chemoattractant protein-3..  FEBS letters,  (21): [PMID:8898111]
6. Van Coillie, E E and 7 more authors..  (1997)  Human monocyte chemotactic protein-2: cDNA cloning and regulated expression of mRNA in mesenchymal cells..  Biochemical and biophysical research communications,  (24): [PMID:9070881]
7. Van Coillie, E E and 7 more authors..  (1997)  The human MCP-2 gene (SCYA8): cloning, sequence analysis, tissue expression, and assignment to the CC chemokine gene contig on chromosome 17q11.2..  Genomics,  (1): [PMID:9119400]

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