货号 (SKU)

包装规格

是否现货

价格

数量

rp147754-10μg

10μg

现货

rp147754-50μg

50μg

现货

rp147754-100μg

100μg

现货

rp147754-1mg

1mg

期货

基本描述

产品名称	Recombinant Human IFN-alpha/beta R1 Protein
别名	重组人IFN-alpha/beta R1蛋白 \| 重组人干扰素α/β受体蛋白
英文别名	alpha-type antiviral protein \| AVP \| beta-type antiviral protein \| CRF2-1 \| Cytokine receptor class-II member 1 \| Cytokine receptor family 2 member 1 \| human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC \| IFN-alpha/beta R1 \| IFN-alpha/beta receptor 1
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity ≥95% SDS-PAGE. Endotoxin level <0.1 EU/µg Function Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves.
生物活性	Measured by its binding ability in a functional ELISA. When Recombinant Human IFN-alpha / beta R1 is present at 10 μg/mL, it binds to Recombinant Human IFN‑ alpha / beta R2 Fc Chimera in the presence of recombinant human IFN-alpha 2. The concentration of Recombinant Human IFN-alpha / beta R2 Fc Chimera that produces 50% of the optimal binding response is approximately 0.5-1.5 μg/mL.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	28-436 aa
序列	KNLKSPQKVEVDIIDDNFILRWNRSDESVGNVTFSFDYQKTGMDNWIKLSGCQNITSTKCNFSSLKLNVYEEIKLRIRAEKENTSSWYEVDSFTPFRKAQIGPPEVHLEAEDKAIVIHISPGTKDSVMWALDGLSFTYSLVIWKNSSGVEERIENIYSRHKIYKLSPETTYCLKVKAALLTSWKIGVYSPVHCIKTTVENELPPPENIEVSVQNQNYVLKWDYTYANMTFQVQWLHAFLKRNPGNHLYKWKQIPDCENVKTTQCVFPQNVFQKGIYLLRVQASDGNNTSFWSEEIKFDTEIQAFLLPPVFNIRSLSDSFHIYIGAPKQSGNTPVIQDYPLIYEIIFWENTSNAERKIIEKKTDVTVPNLKPLTVYCVKARAHTMDEKLNKSSVFSDAVCEKTKPGNTSKHHHHHH
纯度	≥95% (SDS-PAGE)
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P17181
来源	重组表达
预测分子量	49 kDa
SDS-PAGE	78 kDa under reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% trehalose, 5% mannitol, pH7.4.
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。复溶后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human IFN-alpha/beta R1 Protein (rp147754)

Recombinant Human IFN-alpha/beta R1 Protein (rp147754) - SDS-PAGE
2 μg/lane of Recombinant Human IFN-alpha/beta R1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 65 - 85 kDa.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0724754	分析证书	rp147754
ZJ25F0724753	分析证书	rp147754
ZJ25F0724752	分析证书	rp147754

引用文献

1. Zhang G, deWeerd NA, Stifter SA, Liu L, Zhou B, Wang W, Zhou Y, Ying B, Hu X, Matthews AY et al.. (2018) A proline deletion in IFNAR1 impairs IFN-signaling and underlies increased resistance to tuberculosis in humans.. Nat Commun, 9 (1): (85). [PMID:29311663]
2. Zhang Q, Bastard P, Liu Z, Le Pen J, Moncada-Velez M, Chen J, Ogishi M, Sabli IKD, Hodeib S, Korol C et al.. (2020) Inborn errors of type I IFN immunity in patients with life-threatening COVID-19.. Science, 370 (6515): [PMID:32972995]
3. Bello AM, Bende T, Wei L, Wang X, Majchrzak-Kita B, Fish EN, Kotra LP.. (2008) De novo design of nonpeptidic compounds targeting the interactions between interferon-alpha and its cognate cell surface receptor.. J Med Chem, 51 (9): (2734-2743). [PMID:18393399] [10.1021/jm701182y]
4. Lutfalla, G G, Gardiner, K K, Proudhon, D D, Vielh, E E and Uzé, G G.. (1992) The structure of the human interferon alpha/beta receptor gene.. The Journal of biological chemistry, (5): [PMID:1370833]
5. Flores, I I, Mariano, T M TM and Pestka, S S.. (1991) Human interferon omega (omega) binds to the alpha/beta receptor.. The Journal of biological chemistry, (25): [PMID:1834641]
6. Uzé, G G, Lutfalla, G G and Gresser, I I.. (1990) Genetic transfer of a functional human interferon alpha receptor into mouse cells: cloning and expression of its cDNA.. Cell, (26): [PMID:2153461]
7. Colamonici, O O and 8 more authors.. (1994) Direct binding to and tyrosine phosphorylation of the alpha subunit of the type I interferon receptor by p135tyk2 tyrosine kinase.. Molecular and cellular biology, [PMID:7526154]
8. Domanski, P P and 6 more authors.. (1995) Cloning and expression of a long form of the beta subunit of the interferon alpha beta receptor that is required for signaling.. The Journal of biological chemistry, (15): [PMID:7665574]
9. Abramovich, C C and 6 more authors.. (1994) Differential tyrosine phosphorylation of the IFNAR chain of the type I interferon receptor and of an associated surface protein in response to IFN-alpha and IFN-beta.. The EMBO journal, (15): [PMID:7813427]
10. Abramovich, C C, Ratovitski, E E, Lundgren, E E and Revel, M M.. (1994) Identification of mRNAs encoding two different soluble forms of the human interferon alpha-receptor.. FEBS letters, (7): [PMID:8307198]
11. Li, X X, Leung, S S, Kerr, I M IM and Stark, G R GR.. (1997) Functional subdomains of STAT2 required for preassociation with the alpha interferon receptor and for signaling.. Molecular and cellular biology, [PMID:9121453]
12. and Krown, S E SE.. (1987) Interferon treatment of renal cell carcinoma. Current status and future prospects.. Cancer, (1): [PMID:10822464]
13. Hattori, M M and 64 more authors.. (2000) The DNA sequence of human chromosome 21.. Nature, (18): [PMID:10830953]
14. Dupont, Stefan A SA, Goelz, Susan S, Goyal, Jaya J and Green, Marie M.. (2002) Mechanisms for regulation of cellular responsiveness to human IFN-beta1a.. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, [PMID:12034032]
15. Yasuda, Shuhei S and Miyata, Keiji K.. (2002) [Interferon alfacon-1 (Advaferon): a novel synthetic interferon for the treatment of hepatitis C, its pharmacological and clinical profile].. Nihon yakurigaku zasshi. Folia pharmacologica Japonica, [PMID:12528473]
16. Kumar, K G Suresh KG and 5 more authors.. (2003) SCF(HOS) ubiquitin ligase mediates the ligand-induced down-regulation of the interferon-alpha receptor.. The EMBO journal, (15): [PMID:14532120]
17. Kumar, K G Suresh KG, Krolewski, John J JJ and Fuchs, Serge Y SY.. (2004) Phosphorylation and specific ubiquitin acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor.. The Journal of biological chemistry, (5): [PMID:15337770]
18. Dhalluin, Christophe C and 6 more authors.. Structural, kinetic, and thermodynamic analysis of the binding of the 40 kDa PEG-interferon-alpha2a and its individual positional isomers to the extracellular domain of the receptor IFNAR2.. Bioconjugate chemistry, [PMID:15898717]
19. van Koetsveld, Peter M PM and 11 more authors.. (2006) Potent inhibitory effects of type I interferons on human adrenocortical carcinoma cell growth.. The Journal of clinical endocrinology and metabolism, [PMID:16912135]
20. Yano, Hirohisa H and 11 more authors.. (2006) Growth inhibitory effects of pegylated IFN alpha-2b on human liver cancer cells in vitro and in vivo.. Liver international : official journal of the International Association for the Study of the Liver, [PMID:16953837]
21. and Markowitz, Clyde E CE.. (2007) Interferon-beta: mechanism of action and dosing issues.. Neurology, (12): [PMID:17562848]
22. Kumar, K G Suresh KG and 10 more authors.. (2007) Site-specific ubiquitination exposes a linear motif to promote interferon-alpha receptor endocytosis.. The Journal of cell biology, (3): [PMID:18056411]
23. Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973]
24. Claudinon, Julie J and 8 more authors.. (2009) Palmitoylation of interferon-alpha (IFN-alpha) receptor subunit IFNAR1 is required for the activation of Stat1 and Stat2 by IFN-alpha.. The Journal of biological chemistry, (4): [PMID:19561067]
25. Ishii, Koji K and 7 more authors.. (2010) Interferon alpha receptor 2 expression by peripheral blood monocytes in patients with a high viral load of hepatitis C virus genotype 1 showing substitution of amino Acid 70 in the core region.. Intervirology, [PMID:19955815]
26. Thomas, Christoph C and 13 more authors.. (2011) Structural linkage between ligand discrimination and receptor activation by type I interferons.. Cell, (19): [PMID:21854986]
27. Kole, Abhisake A and 6 more authors.. (2013) Type I IFNs regulate effector and regulatory T cell accumulation and anti-inflammatory cytokine production during T cell-mediated colitis.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:23913971]
28. Zheng, Hui H and 10 more authors.. (2013) A BRISC-SHMT complex deubiquitinates IFNAR1 and regulates interferon responses.. Cell reports, (17): [PMID:24075985]
29. Wallweber, Heidi J A HJ, Tam, Christine C, Franke, Yvonne Y, Starovasnik, Melissa A MA and Lupardus, Patrick J PJ.. (2014) Structural basis of recognition of interferon-α receptor by tyrosine kinase 2.. Nature structural & molecular biology, [PMID:24704786]
30. Hu, Xiao X and 9 more authors.. (2015) Pharmacokinetics, pharmacodynamics, and safety of peginterferon beta-1a in subjects with normal or impaired renal function.. Journal of clinical pharmacology, [PMID:25187030]
31. Hu, Xiao X and 6 more authors.. (2015) Pharmacokinetics and pharmacodynamics of peginterferon beta-1a in patients with relapsing-remitting multiple sclerosis in the randomized ADVANCE study.. British journal of clinical pharmacology, [PMID:25265472]
32. Kasper, Lloyd H LH and Reder, Anthony T AT.. (2014) Immunomodulatory activity of interferon-beta.. Annals of clinical and translational neurology, [PMID:25356432]
33. Mastrangeli, Renato R and 10 more authors.. (2016) Biological Functions of Interferon β-1a Are Enhanced By Deamidation.. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, [PMID:27348209]
34. Haji Abdolvahab, M M, Mofrad, M R K MR and Schellekens, H H.. (2016) Interferon Beta: From Molecular Level to Therapeutic Effects.. International review of cell and molecular biology, [PMID:27572132]
35. Hernandez, Nicholas N and 49 more authors.. (2019) Inherited IFNAR1 deficiency in otherwise healthy patients with adverse reaction to measles and yellow fever live vaccines.. The Journal of experimental medicine, (3): [PMID:31270247]
36. Chen, X X, Ji, Z L ZL and Chen, Y Z YZ.. (2002) TTD: Therapeutic Target Database.. Nucleic acids research, (1): [PMID:11752352]

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复溶计算器

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体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Recombinant Human IFN-alpha/beta R1 Protein

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器