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Recombinant Human Progranulin Protein

Recombinant Human Progranulin Protein

级别和纯度:

≥92%(SDS-PAGE)

表达系统: HEK293
Accession #: P28799
蛋白标签: C-His
生物活性: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Progranulin/PGRN, immobilized at 10-40 μg/mL on a nitrocellulose coated plate, is able to significantly enhance neurite outgrowth.
内毒素水平: <0.1 EU/μg

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货号 (SKU)	包装规格	是否现货
rp146631-10μg	10μg	现货
rp146631-50μg	50μg	现货
rp146631-100μg	100μg	现货
rp146631-1mg	1mg	期货

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点击查看蛋白信息: GRN

Accession#: P28799 (1) Gene ID: 2896 (1) Granulin (1) PGRN (1) Progranulin (1)

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基本描述

产品名称	Recombinant Human Progranulin Protein
别名	颗粒体蛋白(GRN)重组蛋白
英文别名	Acrogranin \| Epithelin precursor \| Glycoprotein 88 \| Glycoprotein of 88 Kda \| GP88 \| Granulin precursor \| PC cell-derived growth factor \| PCDGF \| PEPI \| PGRN \| Proepithelin \| Acrogranin \| CLN11 \| GEP \| GP88 \| Granulin A \| Granulin B \| Granulin C \| Granuli
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥92%(SDS-PAGE)
产品介绍	Purity ≥92% (SDS-PAGE) Endotoxin level ＜0.1 EU/μg Function Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.
生物活性	Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Progranulin/PGRN, immobilized at 10-40 μg/mL on a nitrocellulose coated plate, is able to significantly enhance neurite outgrowth.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	18-593 aa
序列	TRCPDGQFCPVACCLDPGGASYSCCRPLLDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRWDAPLRDPALRQLLHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P28799
预测分子量	63 kDa
SDS-PAGE	90-95 kDa, under reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human Progranulin Protein (rp146631)

Recombinant Human Progranulin Protein (rp146631) - SDS-PAGE
2 μg/lane of Recombinant Human Progranulin Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing the band at 70 - 90 kDa under reducing conditions.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0319859	分析证书	rp146631
ZJ25F0319858	分析证书	rp146631
ZJ25F0319860	分析证书	rp146631

引用文献

1. Bhandari, V V and Bateman, A A.. (1992) Structure and chromosomal location of the human granulin gene.. Biochemical and biophysical research communications, (15): [PMID:1417868]
2. Bhandari, V V, Palfree, R G RG and Bateman, A A.. (1992) Isolation and sequence of the granulin precursor cDNA from human bone marrow reveals tandem cysteine-rich granulin domains.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1542665]
3. Plowman, G D GD and 6 more authors.. (1992) The epithelin precursor encodes two proteins with opposing activities on epithelial cell growth.. The Journal of biological chemistry, (25): [PMID:1618805]
4. Bateman, A A, Belcourt, D D, Bennett, H H, Lazure, C C and Solomon, S S.. (1990) Granulins, a novel class of peptide from leukocytes.. Biochemical and biophysical research communications, (31): [PMID:2268320]
5. Kardana, A A, Bagshawe, K D KD, Coles, B B, Read, D D and Taylor, M M.. (1993) Characterisation of UGP and its relationship with beta-core fragment.. British journal of cancer, [PMID:8471426]
6. Tolkatchev, D D, Ng, A A, Vranken, W W and Ni, F F.. (2000) Design and solution structure of a well-folded stack of two beta-hairpins based on the amino-terminal fragment of human granulin A.. Biochemistry, (21): [PMID:10715107]
7. Zhu, Jing J and 10 more authors.. (2002) Conversion of proepithelin to epithelins: roles of SLPI and elastase in host defense and wound repair.. Cell, (13): [PMID:12526812]
8. Baker, Matt M and 26 more authors.. (2006) Mutations in progranulin cause tau-negative frontotemporal dementia linked to chromosome 17.. Nature, (24): [PMID:16862116]
9. Mukherjee, Odity O and 18 more authors.. (2006) HDDD2 is a familial frontotemporal lobar degeneration with ubiquitin-positive, tau-negative inclusions caused by a missense mutation in the signal peptide of progranulin.. Annals of neurology, [PMID:16983685]
10. Mukherjee, Odity O and 14 more authors.. (2008) Molecular characterization of novel progranulin (GRN) mutations in frontotemporal dementia.. Human mutation, [PMID:18183624]
11. Tolkatchev, Dmitri D and 8 more authors.. (2008) Structure dissection of human progranulin identifies well-folded granulin/epithelin modules with unique functional activities.. Protein science : a publication of the Protein Society, [PMID:18359860]
12. Van Damme, Philip P and 8 more authors.. (2008) Progranulin functions as a neurotrophic factor to regulate neurite outgrowth and enhance neuronal survival.. The Journal of cell biology, (7): [PMID:18378771]
13. Guerreiro, Rita J RJ, Washecka, Nicole N, Hardy, John J and Singleton, Andrew A.. (2010) A thorough assessment of benign genetic variability in GRN and MAPT.. Human mutation, [PMID:20020531]
14. Songsrirote, Kriangsak K, Li, Zhi Z, Ashford, David D, Bateman, Andrew A and Thomas-Oates, Jane J.. (2010) Development and application of mass spectrometric methods for the analysis of progranulin N-glycosylation.. Journal of proteomics, (16): [PMID:20188224]
15. Hu, Fenghua F and 8 more authors.. (2010) Sortilin-mediated endocytosis determines levels of the frontotemporal dementia protein, progranulin.. Neuron, (18): [PMID:21092856]
16. Smith, Katherine R KR and 16 more authors.. (2012) Strikingly different clinicopathological phenotypes determined by progranulin-mutation dosage.. American journal of human genetics, (8): [PMID:22608501]
17. Nguyen, Andrew D AD and 7 more authors.. (2013) Secreted progranulin is a homodimer and is not a component of high density lipoproteins (HDL).. The Journal of biological chemistry, (22): [PMID:23364791]
18. Zhou, Xiaolai X and 7 more authors.. (2015) Prosaposin facilitates sortilin-independent lysosomal trafficking of progranulin.. The Journal of cell biology, (14): [PMID:26370502]
19. Jian, Jinlong J and 12 more authors.. (2016) Progranulin Recruits HSP70 to β-Glucocerebrosidase and Is Therapeutic Against Gaucher Disease.. EBioMedicine, [PMID:27789271]
20. Tanaka, Yoshinori Y and 8 more authors.. (2017) Progranulin regulates lysosomal function and biogenesis through acidification of lysosomes.. Human molecular genetics, (1): [PMID:28073925]
21. Beel, Sander S and 7 more authors.. (2017) Progranulin functions as a cathepsin D chaperone to stimulate axonal outgrowth in vivo.. Human molecular genetics, (1): [PMID:28453791]
22. Zhou, Xiaolai X and 10 more authors.. (2017) Impaired prosaposin lysosomal trafficking in frontotemporal lobar degeneration due to progranulin mutations.. Nature communications, (25): [PMID:28541286]
23. Lee, Chris W CW and 12 more authors.. (2017) The lysosomal protein cathepsin L is a progranulin protease.. Molecular neurodegeneration, (25): [PMID:28743268]

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