货号 (SKU)

包装规格

是否现货

价格

数量

rp146365-10μg

10μg

现货

rp146365-50μg

50μg

现货

rp146365-100μg

100μg

现货

rp146365-1mg

1mg

期货

基本描述

产品名称	Recombinant Human GDF-5 Protein
别名	重组人GDF5蛋白 \| 重组人生长分化因子5 \| 重组人 GDF-5 蛋白
英文别名	BMP14 \| BMP-14 \| Cartilage-derived morphogenetic protein 1 \| cartilage-derived morphogenetic protein-1 \| CDMP-1 \| CDMP1 \| LAP4 \| GDF5 \| GDF-5 \| growth differentiation factor 5 \| growth/differentiation factor 5 \| OS5 \| radotermin \| SYNS2 \| BDA1C \| LAP-4 \|
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE&HPLC)
产品介绍	Purity >95% (SDS-PAGE&HPLC) Endotoxin level <0.1 EU/µg Function Could be involved in bone and cartilage formation. Chondrogenic signaling is mediated by the high-affinity receptor BMPR1B.
生物活性	Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/ml, corresponding to a specific activity of >1000 IU/mg.
内毒素水平	<0.1 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	382-501 aa
序列	APLATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR
纯度	>95% (SDS-PAGE&HPLC)
蛋白标签	No tag
无载体	Yes
无动物源	Yes
Accession #	P43026
来源	重组表达
预测分子量	27.1 kDa
SDS-PAGE	27.1 kDa

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from a 0.2 µm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.5 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	自收到之日起 12 个月，-20 至 -70 °C。1 个月，复溶后无菌条件下 2 至 8 °C。3 个月，复溶后在无菌条件下 -20 至 -70 °C。收货后建议分装。避免冻融循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human GDF-5 Protein (rp146365)

Recombinant Human GDF-5 Protein (rp146365) - Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/mL, corresponding to a specific activity of > 1000 IU/mg.

SDS-PAGE: Recombinant Human GDF-5 Protein (rp146365)

Recombinant Human GDF-5 Protein (rp146365) - SDS-PAGE
Recombinant Human GDF-5 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining. Showing a single band at 27.1 kDa.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0809637	分析证书	rp146365
ZJ24F0809635	分析证书	rp146365
ZJ24F0809636	分析证书	rp146365

引用文献

1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J. (2001) The DNA sequence and comparative analysis of human chromosome 20.. Nature, 414 (6866): (865-71). [PMID:11780052]
2. Chang, S C SC and 8 more authors.. (1994) Cartilage-derived morphogenetic proteins. New members of the transforming growth factor-beta superfamily predominantly expressed in long bones during human embryonic development.. The Journal of biological chemistry, (11): [PMID:7961761]
3. Hötten, G G, Neidhardt, H H, Jacobowsky, B B and Pohl, J J.. (1994) Cloning and expression of recombinant human growth/differentiation factor 5.. Biochemical and biophysical research communications, (28): [PMID:7980526]
4. Thomas, J T JT and 5 more authors.. (1996) A human chondrodysplasia due to a mutation in a TGF-beta superfamily member.. Nature genetics, [PMID:8589725]
5. Thomas, J T JT and 7 more authors.. (1997) Disruption of human limb morphogenesis by a dominant negative mutation in CDMP1.. Nature genetics, [PMID:9288098]
6. Triantafilou, K K, Triantafilou, M M and Dedrick, R L RL.. (2001) A CD14-independent LPS receptor cluster.. Nature immunology, [PMID:11276205]
7. Faiyaz-Ul-Haque, M M and 7 more authors.. (2002) Mutation in the cartilage-derived morphogenetic protein-1 (CDMP1) gene in a kindred affected with fibular hypoplasia and complex brachydactyly (DuPan syndrome).. Clinical genetics, [PMID:12121354]
8. Schwabe, Georg C GC and 6 more authors.. (2004) Brachydactyly type C caused by a homozygous missense mutation in the prodomain of CDMP1.. American journal of medical genetics. Part A, (1): [PMID:14735582]
9. Seemann, Petra P and 13 more authors.. (2005) Activating and deactivating mutations in the receptor interaction site of GDF5 cause symphalangism or brachydactyly type A2.. The Journal of clinical investigation, [PMID:16127465]
10. Szczaluba, K K and 5 more authors.. (2005) Du Pan syndrome phenotype caused by heterozygous pathogenic mutations in CDMP1 gene.. American journal of medical genetics. Part A, (1): [PMID:16222676]
11. Dawson, Katherine K and 7 more authors.. (2006) GDF5 is a second locus for multiple-synostosis syndrome.. American journal of human genetics, [PMID:16532400]
12. Wang, Xu X and 13 more authors.. (2006) A novel mutation in GDF5 causes autosomal dominant symphalangism in two Chinese families.. American journal of medical genetics. Part A, (1): [PMID:16892395]
13. Miyamoto, Yoshinari Y and 15 more authors.. (2007) A functional polymorphism in the 5' UTR of GDF5 is associated with susceptibility to osteoarthritis.. Nature genetics, [PMID:17384641]
14. Plöger, Frank F and 7 more authors.. (2008) Brachydactyly type A2 associated with a defect in proGDF5 processing.. Human molecular genetics, (1): [PMID:18203755]
15. Yang, Wei W and 9 more authors.. (2008) Novel point mutations in GDF5 associated with two distinct limb malformations in Chinese: brachydactyly type C and proximal symphalangism.. Journal of human genetics, [PMID:18283415]
16. Sengle, Gerhard G and 7 more authors.. (2008) Targeting of bone morphogenetic protein growth factor complexes to fibrillin.. The Journal of biological chemistry, (16): [PMID:18339631]
17. Douzgou, Sofia S, Lehmann, Katarina K, Mingarelli, Rita R, Mundlos, Stefan S and Dallapiccola, Bruno B.. (2008) Compound heterozygosity for GDF5 in Du Pan type chondrodysplasia.. American journal of medical genetics. Part A, (15): [PMID:18629880]
18. Kotzsch, Alexander A, Nickel, Joachim J, Seher, Axel A, Sebald, Walter W and Müller, Thomas D TD.. (2009) Crystal structure analysis reveals a spring-loaded latch as molecular mechanism for GDF-5-type I receptor specificity.. The EMBO journal, (8): [PMID:19229295]
19. Seemann, Petra P and 19 more authors.. (2009) Mutations in GDF5 reveal a key residue mediating BMP inhibition by NOGGIN.. PLoS genetics, [PMID:19956691]
20. Byrnes, Ashley M AM and 6 more authors.. (2010) Mutations in GDF5 presenting as semidominant brachydactyly A1.. Human mutation, [PMID:20683927]
21. Schwaerzer, Gerburg K GK and 7 more authors.. (2012) New insights into the molecular mechanism of multiple synostoses syndrome (SYNS): mutation within the GDF5 knuckle epitope causes noggin-resistance.. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, [PMID:21976273]
22. Gutiérrez-Amavizca, Bianca Ethel BE and 6 more authors.. (2012) A novel mutation in CDMP1 causes brachydactyly type C with "angel-shaped phalanx". A genotype-phenotype correlation in the mutational spectrum.. European journal of medical genetics, [PMID:22828468]
23. Degenkolbe, Elisa E and 13 more authors.. (2013) A GDF5 point mutation strikes twice--causing BDA1 and SYNS2.. PLoS genetics, [PMID:24098149]
24. Stange, Katja K and 11 more authors.. (2014) Molecular analysis of two novel missense mutations in the GDF5 proregion that reduce protein activity and are associated with brachydactyly type C.. Journal of molecular biology, (23): [PMID:25092592]
25. Al-Qattan, Mohammad M MM, Al-Motairi, Muhammed I MI and Al Balwi, Mohammed A MA.. (2015) Two novel homozygous missense mutations in the GDF5 gene cause brachydactyly type C.. American journal of medical genetics. Part A, [PMID:25820810]
26. Wang, Jian J and 16 more authors.. (2016) A New Subtype of Multiple Synostoses Syndrome Is Caused by a Mutation in GDF6 That Decreases Its Sensitivity to Noggin and Enhances Its Potency as a BMP Signal.. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, [PMID:26643732]

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稀释计算器

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复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Recombinant Human GDF-5 Protein

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器