货号 (SKU)

包装规格

是否现货

价格

数量

rp146167-10μg

10μg

现货

rp146167-50μg

50μg

现货

rp146167-100μg

100μg

现货

rp146167-1mg

1mg

期货

基本描述

产品名称	Recombinant Human Frizzled-4 Protein
别名	卷曲同源物4(FZD4)重组蛋白
英文别名	Fz-4 \| FzE4 \| hFz4 \| CD344 \| CD344 antigen \| EVR1 \| exudative vitreoretinopathy 1 \| FEVR \| frizzled (Drosophila) homolog 4 \| frizzled homolog 4 (Drosophila) \| Frizzled4 \| Frizzled-4 \| Fz-4 \| FZD4 \| FZD4S \| FzE4 \| GPCR \| hFz4 \| MGC34390 \| WNT receptor friz
规格或纯度	ActiBioPure™, 生物活性, 无动物源, Carrier Free, ≥95%(SDS-PAGE)
产品介绍	Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
生物活性	Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing biotinylated rmWnt-5a at 100 ng/mL and rhFrizzled-4/Fc Chimera dilutions at 0.1-2,000 ng/mL, the concentration of rhFrizzled-4/Fc Chimera that produces 50% of the optimal binding response is found to be approximately 15-60 ng/mL.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	37-180 aa
序列	FGDEEERRCDPIRISMCQNLGYNVTKMPNLVGHELQTDAELQLTTFTPLIQYGCSSQLQFFLCSVYVPMCTEKINIPIGPCGGMCLSVKRRCEPVLKEFGFAWPESLNCSKFPPQNDHNHMCMEGPGDEEVPLPHKTPIQPGEEENLYFQGMDPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH
蛋白标签	C-hFc & His
无载体	Yes
无动物源	Yes
Accession #	Q9ULV1
来源	重组表达
预测分子量	44.1 kDa
SDS-PAGE	55 kDa, under reducing conditions; 115 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human Frizzled-4 Protein (rp146167)

Recombinant Human Frizzled-4 Protein (rp146167) - SDS-PAGE
2 μg/lane of Recombinant Human Frizzled-4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 55 kDa under reducing condition and 115 kDa under non-reducing condition.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0319631	分析证书	rp146167
ZJ25F0319630	分析证书	rp146167
ZJ25F0319629	分析证书	rp146167

引用文献

1. Kirikoshi H, Sagara N, Koike J, Tanaka K, Sekihara H, Hirai M, Katoh M. (1999) Molecular cloning and characterization of human Frizzled-4 on chromosome 11q14-q21.. Biochem Biophys Res Commun, 264 (3): (955-61). [PMID:10544037]
2. Xu Q, Wang Y, Dabdoub A, Smallwood PM, Williams J, Woods C, Kelley MW, Jiang L, Tasman W, Zhang K et al.. (2004) Vascular development in the retina and inner ear: control by Norrin and Frizzled-4, a high-affinity ligand-receptor pair.. Cell, 116 (6): (883-95). [PMID:15035989]
3. Robitaille J, MacDonald ML, Kaykas A, Sheldahl LC, Zeisler J, Dubé MP, Zhang LH, Singaraja RR, Guernsey DL, Zheng B, Siebert LF, Hoskin-Mott A, Trese MT, Pimstone SN, Shastry BS, Moon RT, Hayden MR, Goldberg YP, Samuels ME. (2002) Mutant frizzled-4 disrupts retinal angiogenesis in familial exudative vitreoretinopathy.. Nat Genet, 32 (326-330). [PMID:12172548]
4. Yoshida S, Arita R, Yoshida A, Tada H, Emori A, Noda Y, Nakao S, Fujisawa K, Ishibashi T. (2004) Novel mutation in FZD4 gene in a Japanese pedigree with familial exudative vitreoretinopathy.. Am J Ophthalmol, 138 (4): (670-1). [PMID:15488808]
5. Qin M, Hayashi H, Oshima K, Tahira T, Hayashi K, Kondo H. (2005) Complexity of the genotype-phenotype correlation in familial exudative vitreoretinopathy with mutations in the LRP5 and/or FZD4 genes.. Hum Mutat, 26 (2): (104-12). [PMID:15981244]
6. MacDonald ML, Goldberg YP, Macfarlane J, Samuels ME, Trese MT, Shastry BS. (2005) Genetic variants of frizzled-4 gene in familial exudative vitreoretinopathy and advanced retinopathy of prematurity.. Clin Genet, 67 (4): (363-6). [PMID:15733276]
7. Kondo H, Hayashi H, Oshima K, Tahira T, Hayashi K. (2003) Frizzled 4 gene (FZD4) mutations in patients with familial exudative vitreoretinopathy with variable expressivity.. Br J Ophthalmol, 87 (10): (1291-5). [PMID:14507768]
8. Tanaka S, Akiyoshi T, Mori M, Wands JR, Sugimachi K. (1998) A novel frizzled gene identified in human esophageal carcinoma mediates APC/beta-catenin signals.. Proc Natl Acad Sci USA, 95 (17): (10164-9). [PMID:9707618]
9. Yang S, Wu Y, Xu TH, de Waal PW, He Y, Pu M, Chen Y, DeBruine ZJ, Zhang B, Zaidi SA et al.. (2018) Crystal structure of the Frizzled 4 receptor in a ligand-free state.. Nature, 560 (7720): (666-670). [PMID:30135577]
10. Riccio G, Bottone S, La Regina G, Badolati N, Passacantilli S, Rossi GB, Accardo A, Dentice M, Silvestri R, Novellino E et al.. (2018) A Negative Allosteric Modulator of WNT Receptor Frizzled 4 Switches into an Allosteric Agonist.. Biochemistry, 57 (5): (839-851). [PMID:29293331]
11. Generoso SF, Giustiniano M, La Regina G, Bottone S, Passacantilli S, Di Maro S, Cassese H, Bruno A, Mallardo M, Dentice M et al.. (2015) Pharmacological folding chaperones act as allosteric ligands of Frizzled4.. Nat Chem Biol, 11 (4): (280-6). [PMID:25751279]
12. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811]
13. Toomes, Carmel C and 12 more authors.. (2004) Spectrum and frequency of FZD4 mutations in familial exudative vitreoretinopathy.. Investigative ophthalmology & visual science, [PMID:15223780]
14. Omoto, Satoshi S, Hayashi, Takaaki T, Kitahara, Kenji K, Takeuchi, Tomokazu T and Ueoka, Yasuo Y.. (2004) Autosomal dominant familial exudative vitreoretinopathy in two Japanese families with FZD4 mutations (H69Y and C181R).. Ophthalmic genetics, [PMID:15370539]
15. Nallathambi, Jeyabalan J and 5 more authors.. (2006) Identification of novel FZD4 mutations in Indian patients with familial exudative vitreoretinopathy.. Molecular vision, (21): [PMID:17093393]
16. Qin, Minghui M, Kondo, Hiroyuki H, Tahira, Tomoko T and Hayashi, Kenshi K.. (2008) Moderate reduction of Norrin signaling activity associated with the causative missense mutations identified in patients with familial exudative vitreoretinopathy.. Human genetics, [PMID:17955262]
17. Robitaille, Johane M JM and 6 more authors.. (2009) Phenotypic overlap of familial exudative vitreoretinopathy (FEVR) with persistent fetal vasculature (PFV) caused by FZD4 mutations in two distinct pedigrees.. Ophthalmic genetics, [PMID:19172507]
18. Boonstra, F Nienke FN and 10 more authors.. (2009) Clinical and molecular evaluation of probands and family members with familial exudative vitreoretinopathy.. Investigative ophthalmology & visual science, [PMID:19324841]
19. Ells, Anna A and 11 more authors.. (2010) Severe retinopathy of prematurity associated with FZD4 mutations.. Ophthalmic genetics, [PMID:20141357]
20. Nikopoulos, Konstantinos K and 16 more authors.. (2010) Overview of the mutation spectrum in familial exudative vitreoretinopathy and Norrie disease with identification of 21 novel variants in FZD4, LRP5, and NDP.. Human mutation, [PMID:20340138]
21. Hao, Huai-Xiang HX and 18 more authors.. (2012) ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner.. Nature, (29): [PMID:22575959]
22. Capurro, Mariana M, Martin, Tonya T, Shi, Wen W and Filmus, Jorge J.. (2014) Glypican-3 binds to Frizzled and plays a direct role in the stimulation of canonical Wnt signaling.. Journal of cell science, (1): [PMID:24496449]

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体积（添加到小瓶中）

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所需的复溶浓度

Recombinant Human Frizzled-4 Protein

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器