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Recombinant Human Chitotriosidase/CHIT1 Protein

Recombinant Human Chitotriosidase/CHIT1 Protein

级别和纯度:

≥90%(SDS-PAGE)

表达系统: HEK293
Accession #: Q13231
蛋白标签: C-10His
生物活性: Measured by its ability to cleave 4-Methylumbelliferyl-beta -D-N,N’,N”-triacetylchitotriose (4-MU-TACT). The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.
内毒素水平: <0.1 EU/μg

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货号 (SKU)	包装规格	是否现货
rp144328-10μg	10μg	现货
rp144328-50μg	50μg	现货
rp144328-100μg	100μg	现货
rp144328-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CHIT1

Accession#: Q13231 (1) CHIT1 (1) Chitotriosidase (1) Gene ID: 11118 (2)

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基本描述

产品名称	Recombinant Human Chitotriosidase/CHIT1 Protein
别名	几丁质酶1(CHIT1)重组蛋白
英文别名	Chitinase-1 \| EC 3.2.1.14 \| CHIT1 \| CHI3 \| CHITCHI3 \| chitinase 1 (chitotriosidase) \| chitinase-1 \| Chitotriosidase \| chitotriosidase-1 \| EC 3.2.1.14 \| FLJ00314 \| MGC125322 \| plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase \| CHIT \| CHITD
规格或纯度	ActiBioPure™, 生物活性, 无动物源, Carrier Free, ≥90%(SDS-PAGE)
产品介绍	Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.
生物活性	Measured by its ability to cleave 4-Methylumbelliferyl-beta -D-N,N’,N”-triacetylchitotriose (4-MU-TACT). The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	22-466 aa
序列	AKLVCYFTNWAQYRQGEARFLPKDLDPSLCTHLIYAFAGMTNHQLSTTEWNDETLYQEFNGLKKMNPKLKTLLAIGGWNFGTQKFTDMVATANNRQTFVNSAIRFLRKYSFDGLDLDWEYPGSQGSPAVDKERFTTLVQDLANAFQQEAQTSGKERLLLSAAVPAGQTYVDAGYEVDKIAQNLDFVNLMAYDFHGSWEKVTGHNSPLYKRQEESGAAASLNVDAAVQQWLQKGTPASKLILGMPTYGRSFTLASSSDTRVGAPATGSGTPGPFTKEGGMLAYYEVCSWKGATKQRIQDQKVPYIFRDNQWVGFDDVESFKTKVSYLKQKGLGGAMVWALDLDDFAGFSCNQGRYPLIQTLRQELSLPYLPSGTPELEVPKPGQPSEPEHGPSPGQDTFCQGKADGLYPNPRERSSFYSCAAGRLFQQSCPTGLVFSNSCKCCTWNHHHHHHHHHH
蛋白标签	C-10His
无载体	Yes
无动物源	Yes
Accession #	Q13231
来源	重组表达
预测分子量	51 kDa
SDS-PAGE	47 - 55 kDa, under reducing conditions; 45 - 52 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human Chitotriosidase/CHIT1 Protein (rp144328)

Recombinant Human Chitotriosidase/CHIT1 Protein (rp144328) - SDS-PAGE
3 μg/lane of Recombinant Human Chitotriosidase/CHIT1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 47 - 55 kDa under reducing condition and 45 - 52 kDa under non-reducing condition.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F1215547	分析证书	rp144328
ZJ24F1215548	分析证书	rp144328
ZJ24F1215549	分析证书	rp144328

引用文献

1. Mazur M, Olczak J, Olejniczak S, Koralewski R, Czestkowski W, Jedrzejczak A, Golab J, Dzwonek K, Dymek B, Sklepkiewicz PL et al.. (2018) Targeting Acidic Mammalian chitinase Is Effective in Animal Model of Asthma.. J Med Chem, 61 (3): (695-710). [PMID:29283260]
2. Koralewski R, Dymek B, Mazur M, Sklepkiewicz P, Olejniczak S, Czestkowski W, Matyszewski K, Andryianau G, Niedziejko P, Kowalski M et al.. (2020) Discovery of OATD-01, a First-in-Class Chitinase Inhibitor as Potential New Therapeutics for Idiopathic Pulmonary Fibrosis.. J Med Chem, 63 (24): (15527-15540). [PMID:33078933]
3. Mazur M, Dymek B, Koralewski R, Sklepkiewicz P, Olejniczak S, Mazurkiewicz M, Piotrowicz M, Salamon M, Jędrzejczak K, Zagozdzon A, Czestkowski W, Matyszewski K, Borek B, Bartoszewicz A, Pluta E, Rymaszewska A, Mozga W, Stefaniak F, Dobrzański P, Dzwonek K, Golab J, Golebiowski A, Olczak J.. (2019) Development of Dual Chitinase Inhibitors as Potential New Treatment for Respiratory System Diseases.. J Med Chem, 62 (15): (7126-7145). [PMID:31291098] [10.1021/acs.jmedchem.9b00681]
4. Jiang X, Kumar A, Motomura Y, Liu T, Zhou Y, Moro K, Zhang KYJ, Yang Q.. (2020) A Series of Compounds Bearing a Dipyrido-Pyrimidine Scaffold Acting as Novel Human and Insect Pest Chitinase Inhibitors.. J Med Chem, 63 (3): (987-1001). [PMID:31928006] [10.1021/acs.jmedchem.9b01154]
5. Andryianau G, Kowalski M, Piotrowicz MC, Rajkiewicz AA, Dymek B, Sklepkiewicz PL, Pluta E, Stefaniak F, Czestkowski W, Olejniczak S, Mazur M, Niedziejko P, Koralewski R, Matyszewski K, Gruza M, Zagozdzon A, Salamon M, Rymaszewska A, Welzer M, Dzwonek K, Golab J, Olczak J, Bartoszewicz A, Golebiowski A.. (2020) Benzoxazepine-Derived Selective, Orally Bioavailable Inhibitor of Human Acidic Mammalian Chitinase.. ACS Med Chem Lett, 11 (6): (1228-1235). [PMID:32551005] [10.1021/acsmedchemlett.0c00092]
6. Boot, R G RG, Renkema, G H GH, Strijland, A A, van Zonneveld, A J AJ and Aerts, J M JM.. (1995) Cloning of a cDNA encoding chitotriosidase, a human chitinase produced by macrophages.. The Journal of biological chemistry, (3): [PMID:7592832]
7. Renkema, G H GH, Boot, R G RG, Muijsers, A O AO, Donker-Koopman, W E WE and Aerts, J M JM.. (1995) Purification and characterization of human chitotriosidase, a novel member of the chitinase family of proteins.. The Journal of biological chemistry, (3): [PMID:7836450]
8. Boot, R G RG and 7 more authors.. (1998) The human chitotriosidase gene. Nature of inherited enzyme deficiency.. The Journal of biological chemistry, (2): [PMID:9748235]
9. Fusetti, Fabrizia F and 7 more authors.. (2002) Structure of human chitotriosidase. Implications for specific inhibitor design and function of mammalian chitinase-like lectins.. The Journal of biological chemistry, (12): [PMID:11960986]
10. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414]
11. Grace, Marie E ME, Balwani, Manisha M, Nazarenko, Irina I, Prakash-Cheng, Ainu A and Desnick, Robert J RJ.. (2007) Type 1 Gaucher disease: null and hypomorphic novel chitotriosidase mutations-implications for diagnosis and therapeutic monitoring.. Human mutation, [PMID:17464953]
12. Andersen, Ole A OA, Nathubhai, Amit A, Dixon, Mark J MJ, Eggleston, Ian M IM and van Aalten, Daan M F DM.. (2008) Structure-based dissection of the natural product cyclopentapeptide chitinase inhibitor argifin.. Chemistry & biology, [PMID:18355729]
13. Bussink, Anton P AP and 8 more authors.. (2009) Common G102S polymorphism in chitotriosidase differentially affects activity towards 4-methylumbelliferyl substrates.. The FEBS journal, [PMID:19725875]
14. Sutherland, Tara E TE and 6 more authors.. (2011) Analyzing airway inflammation with chemical biology: dissection of acidic mammalian chitinase function with a selective drug-like inhibitor.. Chemistry & biology, (27): [PMID:21609838]

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