Recombinant Human CD3 delta/CD3d Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P04234-1
蛋白标签: C-His
生物活性: Measured by its binding ability in a functional ELISA. When Recombinant Human CD3 delta/CD3d is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Recombinant Human CD3E Fc Chimera Biotinylated Protein that produces 50% of the optimal binding response is approximately 1.0-6.0 μg/mL.
内毒素水平: <0.1 EU/μg

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
rp143990-10μg	10μg	现货
rp143990-50μg	50μg	现货
rp143990-100μg	100μg	现货
rp143990-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CD3D

Accession#:P04234 (5) CD3 (46) CD3 delta (1) CD3D (5) Gene ID:915 (5)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本描述

产品名称	Recombinant Human CD3 delta/CD3d Protein
别名	重组人CD3 delta/CD3d蛋白 \| 重组人CD3d分子蛋白
英文别名	T-cell receptor T3 delta chain \| CD3 delta \| CD3d antigen \| CD3d antigen, delta polypeptide (TiT3 complex) \| CD3d molecule, delta (CD3-TCR complex) \| CD3D \| CD3-DELTA \| T3DOKT3, delta chain \| T-cell receptor T3 delta chain \| CD3 antigen, delta subunit \| T
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, ≥95%(SDS-PAGE)
产品介绍	Function The CD3 complex mediates signal transduction.
生物活性	Measured by its binding ability in a functional ELISA. When Recombinant Human CD3 delta/CD3d is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Recombinant Human CD3E Fc Chimera Biotinylated Protein that produces 50% of the optimal binding response is approximately 1.0-6.0 μg/mL.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	22-105 aa
序列	FKIPIEELEDRVFVNCNTSITWVEGTVGTLLSDITRLDLGKRILDPRGIYRCNGTDIYKDKESTVQVHYRMCQSCVELDPATVAHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P04234-1
来源	重组表达
预测分子量	10 kDa
SDS-PAGE	17 kDa under reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。复溶后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human CD3 delta/CD3d Protein (rp143990)

Recombinant Human CD3 delta/CD3d Protein (rp143990) - SDS-PAGE
2 μg/lane of Recombinant Human CD3 delta/CD3d Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 17 - 27 kDa.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0723534	分析证书	rp143990
ZJ25F0723533	分析证书	rp143990
ZJ25F0723532	分析证书	rp143990

引用文献

1. de Saint Basile G, Geissmann F, Flori E, Uring-Lambert B, Soudais C, Cavazzana-Calvo M, Durandy A, Jabado N, Fischer A, Le Deist F. (2004) Severe combined immunodeficiency caused by deficiency in either the delta or the epsilon subunit of CD3.. J Clin Invest, 114 (10): (1512-7). [PMID:15546002]
2. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811]
3. Phillips, J H JH and 5 more authors.. (1992) Ontogeny of human natural killer (NK) cells: fetal NK cells mediate cytolytic function and express cytoplasmic CD3 epsilon,delta proteins.. The Journal of experimental medicine, (1): [PMID:1372642]
4. Barber, E K EK, Dasgupta, J D JD, Schlossman, S F SF, Trevillyan, J M JM and Rudd, C E CE.. (1989) The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2470098]
5. Alexander, D D and 5 more authors.. (1989) Dephosphorylation of the human T lymphocyte CD3 antigen.. European journal of biochemistry, (15): [PMID:2540970]
6. van den Elsen, P P, Georgopoulos, K K, Shepley, B A BA, Orkin, S S and Terhorst, C C.. (1986) Exon/intron organization of the genes coding for the delta chains of the human and murine T-cell receptor/T3 complex.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2939461]
7. Tunnacliffe, A A, Sims, J E JE and Rabbitts, T H TH.. (1986) T3 delta pre-mRNA is transcribed from a non-TATA promoter and is alternatively spliced in human T cells.. The EMBO journal, [PMID:3488209]
8. van den Elsen, P P and 6 more authors.. (1984) Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex.. Nature, [PMID:6095101]
9. Wilde, M I MI and Goa, K L KL.. (1996) Muromonab CD3: a reappraisal of its pharmacology and use as prophylaxis of solid organ transplant rejection.. Drugs, [PMID:8861551]
10. Doucey, Marie-Agnès MA and 7 more authors.. (2003) CD3 delta establishes a functional link between the T cell receptor and CD8.. The Journal of biological chemistry, (31): [PMID:12215456]
11. Salomon, Arthur R AR and 10 more authors.. (2003) Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry.. Proceedings of the National Academy of Sciences of the United States of America, (21): [PMID:12522270]
12. Dadi, Harjit K HK, Simon, Amos J AJ and Roifman, Chaim M CM.. (2003) Effect of CD3delta deficiency on maturation of alpha/beta and gamma/delta T-cell lineages in severe combined immunodeficiency.. The New England journal of medicine, (6): [PMID:14602880]
13. Jin, Pei P and 7 more authors.. (2004) PCR isolation and cloning of novel splice variant mRNAs from known drug target genes.. Genomics, [PMID:15028279]
14. Brill, Laurence M LM and 5 more authors.. (2004) Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.. Analytical chemistry, (15): [PMID:15144186]
15. Arnett, Kelly L KL, Harrison, Stephen C SC and Wiley, Don C DC.. (2004) Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.. Proceedings of the National Academy of Sciences of the United States of America, (16): [PMID:15534202]
16. Yu, Grace P and 13 more authors.. (2011) Genotype, phenotype, and outcomes of nine patients with T-B+NK+ SCID.. Pediatric transplantation, [PMID:21883749]
17. Zugmaier, Gerhard G, Klinger, Matthias M, Schmidt, Margit M and Subklewe, Marion M.. (2015) Clinical overview of anti-CD19 BiTE(®) and ex vivo data from anti-CD33 BiTE(®) as examples for retargeting T cells in hematologic malignancies.. Molecular immunology, [PMID:25883042]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度