Recombinant Human Fc gamma RIIA/CD32a Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P12318
蛋白标签: C-His
生物活性: Immobilized recombinant human CD32a at 1 μg/mL (100 μL/well) can bind biotinylated human IgG1 with a linear range of 30-250 ng/mL.
内毒素水平: <0.1 EU/μg

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货号 (SKU)	包装规格	是否现货
rp143958-10μg	10μg	现货
rp143958-50μg	50μg	现货
rp143958-100μg	100μg	现货
rp143958-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: FCGR2A

Accession#:P12318 (8) CD32 (15) CD32A (7) Fc gamma RIIA (1) Fcgr2a (11) Gene ID:2212 (8)

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基本描述

产品名称	Recombinant Human Fc gamma RIIA/CD32a Protein
别名	重组人CD32A蛋白 \| 重组人 Fc gamma RIIA/CD32a 蛋白
英文别名	CDw32 \| Fc-gamma RII-a \| Fc-gamma-RIIa \| FcRII-a \| IgG Fc receptor II-a \| CD32 \| CD32 antigen \| CDw32 \| Fc fragment of IgG, low affinity IIa, receptor (CD32) \| Fc fragment of IgG, low affinity IIa, receptor for (CD32) \| Fc gamma RII a \| Fc gamma RIIa \| Fc
规格或纯度	无动物源, Carrier Free, 生物活性, ActiBioPure™, ≥95%(SDS-PAGE)
产品介绍	Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
生物活性	Immobilized recombinant human CD32a at 1 μg/mL (100 μL/well) can bind biotinylated human IgG1 with a linear range of 30-250 ng/mL.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	36-218 aa (H167R)
序列	AAPPKAVLKLEPPWINVLQEDSVTLTCQGARSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIMLRCHSWKDKPLVKVTFFQNGKSQKFSRLDPTFSIPQANHSHSGDYHCTGNIGYTLFSSKPVTITVQVPSMGSSSPMGIHHHHHH
纯度	≥95% (SDS-PAGE)
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P12318
来源	重组表达
预测分子量	21 kDa
SDS-PAGE	28 - 35 kDa, under reducing conditions; 28 - 35 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human Fc gamma RIIA/CD32a Protein (rp143958)

Recombinant Human Fc gamma RIIA/CD32a Protein (rp143958) - SDS-PAGE
3 μg/lane of Recombinant Human Fc gamma RIIA/CD32a Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 28 - 35 kDa.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F1215504	分析证书	rp143958
ZJ24F1215502	分析证书	rp143958
ZJ24F1215503	分析证书	rp143958

引用文献

1. and Seki, T T.. (1989) Identification of multiple isoforms of the low-affinity human IgG Fc receptor.. Immunogenetics, [PMID:2526077]
2. Brooks, D G DG, Qiu, W Q WQ, Luster, A D AD and Ravetch, J V JV.. (1989) Structure and expression of human IgG FcRII(CD32). Functional heterogeneity is encoded by the alternatively spliced products of multiple genes.. The Journal of experimental medicine, (1): [PMID:2529342]
3. Stuart, S G SG and 6 more authors.. (1987) Isolation and expression of cDNA clones encoding a human receptor for IgG (Fc gamma RII).. The Journal of experimental medicine, (1): [PMID:2824655]
4. Hibbs, M L ML, Bonadonna, L L, Scott, B M BM, McKenzie, I F IF and Hogarth, P M PM.. (1988) Molecular cloning of a human immunoglobulin G Fc receptor.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2965389]
5. Stengelin, S S, Stamenkovic, I I and Seed, B B.. (1988) Isolation of cDNAs for two distinct human Fc receptors by ligand affinity cloning.. The EMBO journal, [PMID:3402431]
6. Hamada, F F, Aoki, M M, Akiyama, T T and Toyoshima, K K.. (1993) Association of immunoglobulin G Fc receptor II with Src-like protein-tyrosine kinase Fgr in neutrophils.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:8327512]
7. Salmon, J E JE and 8 more authors.. (1996) Fc gamma RIIA alleles are heritable risk factors for lupus nephritis in African Americans.. The Journal of clinical investigation, (1): [PMID:8636449]
8. Bewarder, N N and 6 more authors.. (1996) In vivo and in vitro specificity of protein tyrosine kinases for immunoglobulin G receptor (FcgammaRII) phosphorylation.. Molecular and cellular biology, [PMID:8756631]
9. Maxwell, K F KF and 6 more authors.. (1999) Crystal structure of the human leukocyte Fc receptor, Fc gammaRIIa.. Nature structural biology, [PMID:10331870]
10. Powell, M S MS and 8 more authors.. (1999) Biochemical analysis and crystallisation of Fc gamma RIIa, the low affinity receptor for IgG.. Immunology letters, (3): [PMID:10397151]
11. Pengal, Ruma A RA and 5 more authors.. (2003) SHIP-2 inositol phosphatase is inducibly expressed in human monocytes and serves to regulate Fcgamma receptor-mediated signaling.. The Journal of biological chemistry, (20): [PMID:12690104]
12. Lin, Thomas S TS and 9 more authors.. (2005) FCGR3A and FCGR2A polymorphisms may not correlate with response to alemtuzumab in chronic lymphocytic leukemia.. Blood, (1): [PMID:15217834]
13. Criswell, Lindsey A LA and 11 more authors.. (2004) The influence of genetic variation in the HLA-DRB1 and LTA-TNF regions on the response to treatment of early rheumatoid arthritis with methotrexate or etanercept.. Arthritis and rheumatism, [PMID:15457442]
14. Hughes, L B LB and 7 more authors.. (2004) Genetic risk factors for infection in patients with early rheumatoid arthritis.. Genes and immunity, [PMID:15526004]
15. Wang, Yaning Y, Fei, David D, Vanderlaan, Martin M and Song, An A.. (2004) Biological activity of bevacizumab, a humanized anti-VEGF antibody in vitro.. Angiogenesis, [PMID:15886877]
16. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414]
17. Zhang, Wu W and 12 more authors.. (2007) FCGR2A and FCGR3A polymorphisms associated with clinical outcome of epidermal growth factor receptor expressing metastatic colorectal cancer patients treated with single-agent cetuximab.. Journal of clinical oncology : official journal of the American Society of Clinical Oncology, (20): [PMID:17704420]
18. Lu, Jinghua J and 5 more authors.. (2008) Structural recognition and functional activation of FcgammaR by innate pentraxins.. Nature, (18): [PMID:19011614]

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