Recombinant Human CD31/PECAM-1 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P16284
蛋白标签: C-His
生物活性: 1. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells in the presence of 10 μg/mL PHA. The ED₅₀ value was lower than 5 ng/mL. 2. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to CD31 coated plates (5μg/mL, 100 μL/well) in the presence of 10 μg/mL PHA, approximately 60-70% will adhere after 30 minutes at 37℃.
内毒素水平: <0.1 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp143954-10μg	10μg	现货
rp143954-50μg	50μg	现货
rp143954-100μg	100μg	现货
rp143954-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: PECAM1

Accession#:P16284 (8) CD31 (0) Gene ID:5175 (8) PECAM1 (9)

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基本描述

产品名称	Recombinant Human CD31/PECAM-1 Protein
别名	重组人血小板内皮细胞粘附分子1 \| 重组人 CD31/PECAM-1 蛋白
英文别名	adhesion molecule \| CD31 antigen \| CD31 \| CD31/EndoCAM \| EndoCAM \| FLJ34100 \| FLJ58394 \| GPIIA' \| PECA1 \| PECAM1 \| PECAM-1 \| PECAM-1, CD31/EndoCAM \| platelet endothelial cell adhesion molecule \| platelet endothelial cell adhesion molecule-1 \| platelet/end
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Purity >95% SDS-PAGE. Function Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). Post-translational Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
生物活性	1. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells in the presence of 10 μg/mL PHA. The ED₅₀ value was lower than 5 ng/mL. 2. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to CD31 coated plates (5μg/mL, 100 μL/well) in the presence of 10 μg/mL PHA, approximately 60-70% will adhere after 30 minutes at 37℃.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	28-601 aa
序列	QENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQHQMLFYKDDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQVLVEGVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELVTVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKAIVAHNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSKPELESSFTHLDQGERLNLSCSIPGAPPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFEVIKGQTIEVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKASKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKHHHHHH
纯度	＞95%(SDS-PAGE)
蛋白标签	C-His
蛋白长度	Protein fragment
无载体	Yes
无动物源	Yes
Accession #	P16284
来源	重组表达
预测分子量	64 kDa
SDS-PAGE	94.5 kDa, under reducing conditions; 85.9 kDa, under non-reducing conditions.

储存与运输

应用	Active,SDS-PAGE
物理形态	冻干(Lyophilized)
储存缓冲液	10mM PBS(PH7.4), containing 5% trehalose, 5% mannitol
复溶	Reconstitute in water to a concentration of 0.1-1.0 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human CD31/PECAM-1 Protein (rp143954)

Recombinant Human CD31/PECAM-1 Protein (rp143954) - Protein Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells in the presence of 10 μg/mL PHA. The ED₅₀ value was lower than 5 ng/mL.

Protein Bioactivity: Recombinant Human CD31/PECAM-1 Protein (rp143954)

Recombinant Human CD31/PECAM-1 Protein (rp143954) - Protein Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to CD31 coated plates (5μg/mL, 100 μL/well) in the presence of 10 μg/mL PHA, approximately 60-70% will adhere after 30 minutes at 37℃.

SDS-PAGE: Recombinant Human CD31/PECAM-1 Protein (rp143954)

Recombinant Human CD31/PECAM-1 Protein (rp143954) - SDS-PAGE
3μg/lane of Recombinant Human CD31/PECAM-1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 94.5 kDa under reducing conditions and 85.9 kDa under non-reducing conditions.

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找到6个结果

批号(Lot Number)	证书类型	货号
ZJ24F1215129	分析证书	rp143954
ZJ24F1215127	分析证书	rp143954
ZJ24F1215128	分析证书	rp143954
ZJ23F0600494	分析证书	rp143954
ZJ23F0600496	分析证书	rp143954
ZJ23F0600495	分析证书	rp143954

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Recombinant Human CD31/PECAM-1 Protein

库存信息

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基本描述

储存与运输

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质检证书（CoA,COO,BSE/TSE 和分析图谱)

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