货号 (SKU)

包装规格

是否现货

价格

数量

rp143209-GMP-25μg

25μg

现货

rp143209-GMP-1mg

1mg

期货

基本描述

产品名称	Recombinant Human BDNF GMP Protein
别名	重组人BDNF蛋白 \| 重组人脑源性神经营养因子蛋白
英文别名	Abrineurin \| BDNF \| Abrineurin \| ANON2 \| BDNF \| brain-derived neurotrophic factor \| BULN2 \| MGC34632 \| Neurotrophin \| Abrineurin \| ANON2 \| BDNF \| brain-derived neurotrophic factor \| BULN2 \| MGC34632 \| Neurotrophin
规格或纯度	无动物源, 无载体, GMP, 生物活性, ActiBioPure™, 高性能, 无菌, ≥70%(SDS-PAGE)
生化机理	Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
生物活性	The activity is determined by the dose-dependent proliferation of C6 cell sand is typically between 0.5-1.5 ug/mL. Corresponding to a specific Activity of 1 x 10^3 units/mg.
内毒素水平	<10 EU/mg
表达系统	CHO
种属	人(Human)
氨基酸	129-247 aa
序列	HSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR
蛋白标签	No tag
N-末端序列	Met-Arg134-Arg-Gly-Glu-Leu-Ser-Val-(Cys)-Asp
无载体	Yes
无动物源	Yes
Accession #	P23560-1
来源	重组表达
预测分子量	13.5 kDa
SDS-PAGE	12 kDa, under reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from a 0.22 μm filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH7.4.
复溶	It is recommended to redissolve in sterile deionized water.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	长期储存-20℃~-80℃（36个月）；复溶后建议分装，避免反复冻融。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human BDNF GMP Protein (rp143209)

Recombinant Human BDNF GMP Protein (rp143209) - SDS-PAGE
2 μg/lane of Recombinant Human BDNF GMP Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 13.5 kDa.

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引用文献

1. Shintani, A A, Ono, Y Y, Kaisho, Y Y and Igarashi, K K.. (1992) Characterization of the 5'-flanking region of the human brain-derived neurotrophic factor gene.. Biochemical and biophysical research communications, (15): [PMID:1339267]
2. Maisonpierre, P C PC and 8 more authors.. (1991) Human and rat brain-derived neurotrophic factor and neurotrophin-3: gene structures, distributions, and chromosomal localizations.. Genomics, [PMID:1889806]
3. Hallböök, F F, Ibáñez, C F CF and Persson, H H.. (1991) Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary.. Neuron, [PMID:2025430]
4. Jones, K R KR and Reichardt, L F LF.. (1990) Molecular cloning of a human gene that is a member of the nerve growth factor family.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2236018]
5. Robinson, R C RC, Radziejewski, C C, Stuart, D I DI and Jones, E Y EY.. (1995) Structure of the brain-derived neurotrophic factor/neurotrophin 3 heterodimer.. Biochemistry, (4): [PMID:7703225]
6. Rosenfeld, R D RD and 7 more authors.. (1995) Purification and identification of brain-derived neurotrophic factor from human serum.. Protein expression and purification, [PMID:8527932]
7. Cargill, M M and 17 more authors.. (1999) Characterization of single-nucleotide polymorphisms in coding regions of human genes.. Nature genetics, [PMID:10391209]
8. Robinson, R C RC and 8 more authors.. (1999) The structures of the neurotrophin 4 homodimer and the brain-derived neurotrophic factor/neurotrophin 4 heterodimer reveal a common Trk-binding site.. Protein science : a publication of the Protein Society, [PMID:10631974]
9. Mowla, S J SJ and 6 more authors.. (2001) Biosynthesis and post-translational processing of the precursor to brain-derived neurotrophic factor.. The Journal of biological chemistry, (20): [PMID:11152678]
10. Murphy, W J WJ and 5 more authors.. (2001) Molecular phylogenetics and the origins of placental mammals.. Nature, (1): [PMID:11214319]
11. Weese-Mayer, Debra E DE, Bolk, Stacey S, Silvestri, Jean M JM and Chakravarti, Aravinda A.. (2002) Idiopathic congenital central hypoventilation syndrome: evaluation of brain-derived neurotrophic factor genomic DNA sequence variation.. American journal of medical genetics, (1): [PMID:11840487]
12. Egan, Michael F MF and 11 more authors.. (2003) The BDNF val66met polymorphism affects activity-dependent secretion of BDNF and human memory and hippocampal function.. Cell, (24): [PMID:12553913]
13. Hall, Diana D, Dhilla, Alefiya A, Charalambous, Anna A, Gogos, Joseph A JA and Karayiorgou, Maria M.. (2003) Sequence variants of the brain-derived neurotrophic factor (BDNF) gene are strongly associated with obsessive-compulsive disorder.. American journal of human genetics, [PMID:12836135]
14. Ribasés, M M and 9 more authors.. (2003) Met66 in the brain-derived neurotrophic factor (BDNF) precursor is associated with anorexia nervosa restrictive type.. Molecular psychiatry, [PMID:12888803]
15. Sasaki, Ayako A and 13 more authors.. (2003) Molecular analysis of congenital central hypoventilation syndrome.. Human genetics, [PMID:14566559]
16. Ribasés, Marta M and 27 more authors.. (2004) Association of BDNF with anorexia, bulimia and age of onset of weight loss in six European populations.. Human molecular genetics, (15): [PMID:15115760]
17. Liu, Qing-Rong QR and 9 more authors.. (2005) Human brain derived neurotrophic factor (BDNF) genes, splicing patterns, and assessments of associations with substance abuse and Parkinson's Disease.. American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, (5): [PMID:15666411]
18. Pruunsild, Priit P, Kazantseva, Anna A, Aid, Tamara T, Palm, Kaia K and Timmusk, Tõnis T.. (2007) Dissecting the human BDNF locus: bidirectional transcription, complex splicing, and multiple promoters.. Genomics, [PMID:17629449]
19. Han, Joan C JC and 11 more authors.. (2008) Brain-derived neurotrophic factor and obesity in the WAGR syndrome.. The New England journal of medicine, (28): [PMID:18753648]
20. Mandel, A L; Ozdener, H and Utermohlen, V.. (2009) Identification of pro- and mature brain-derived neurotrophic factor in human saliva.. Archives of oral biology, [PMID:19467646]
21. Autry, Anita E AE and Monteggia, Lisa M LM.. (2012) Brain-derived neurotrophic factor and neuropsychiatric disorders.. Pharmacological reviews, [PMID:22407616]
22. Travaglia, Alessio A and 5 more authors.. (2012) Copper, BDNF and Its N-terminal domain: inorganic features and biological perspectives.. Chemistry (Weinheim an der Bergstrasse, Germany), (3): [PMID:23135810]
23. Haile, C N CN and 11 more authors.. (2014) Plasma brain derived neurotrophic factor (BDNF) and response to ketamine in treatment-resistant depression.. The international journal of neuropsychopharmacology, [PMID:24103211]
24. Glerup, Simon S and 22 more authors.. (2014) SorCS2 regulates dopaminergic wiring and is processed into an apoptotic two-chain receptor in peripheral glia.. Neuron, (4): [PMID:24908487]
25. Harripaul, R R and 31 more authors.. (2018) Mapping autosomal recessive intellectual disability: combined microarray and exome sequencing identifies 26 novel candidate genes in 192 consanguineous families.. Molecular psychiatry, [PMID:28397838]
26. Giza, Joanna I and 13 more authors.. (2018) The BDNF Val66Met Prodomain Disassembles Dendritic Spines Altering Fear Extinction Circuitry and Behavior.. Neuron, (11): [PMID:29909994]
27. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334]
28. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al.. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs.. Nat Genet, 36 (1): (40-5). [PMID:14702039]
29. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811]

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Recombinant Human BDNF GMP Protein

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