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Recombinant Human Beta-Defensin 3 Protein

Recombinant Human Beta-Defensin 3 Protein

级别和纯度:

≥98%(SDS-PAGE&HPLC)
0.5M in TMF

表达系统: E. coli
Accession #: P81534
蛋白标签: No tag
生物活性: Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 30 μg/ml.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp143202-10μg	10μg	现货
rp143202-20μg	20μg	期货
rp143202-50μg	50μg	现货
rp143202-100μg	100μg	现货
rp143202-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: DEFB103A

Accession#:P81534 (1) Beta-Defensin 3 (1) DEFB103B (1) Gene ID:55894 (1)

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基本描述

产品名称	Recombinant Human Beta-Defensin 3 Protein
别名	重组人BD-3蛋白 \| 重组人类 β-防御素 3 蛋白
英文别名	BD14 \| BD3 \| BD-3 \| beta-defensin 103 \| betaDefensin 3 \| beta-Defensin 3 \| DEFB103 \| DEFB103A \| DEFB103B \| DEFB3 \| DEFB-3 \| DEFB3DEFB103A \| Defensin, beta 103 \| defensin, beta 103B \| Defensin-like protein \| HBD-3 \| HBD3 \| HBP3 \| HBP-3 \| BD-3 \| Beta-defens
规格或纯度	无动物源, Carrier Free, 生物活性, ActiBioPure™, Azide Free, ≥98%(SDS-PAGE&HPLC), 0.5M in TMF
产品介绍	Purity > 98% by SDS-PAGE and HPLC analyses. Function Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistent E.faecium. No significant hemolytic activity was observe. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human β-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because β-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane.
生物活性	Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 30 μg/ml.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	23-67 aa
序列	GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK
纯度	＞98%(SDS-PAGE, HPLC)
蛋白标签	No tag
蛋白长度	Full length protein
无载体	Yes
无动物源	Yes
Accession #	P81534
来源	重组表达
预测分子量	5.2 kDa

储存与运输

应用	Active, SDS-PAGE
物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
浓度	0.5M in TMF
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	使用手动除霜冷冻机，避免反复冻融循环。自收到之日起 12 个月内，-20 至 -70 ℃（以提供时为准）；1 个月内，复溶后在无菌条件下 2 至 8 ℃；3 个月内，复溶后在无菌条件下 -20 至 -70 ℃。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human Beta-Defensin 3 Protein (rp143202)

Recombinant Human Beta-Defensin 3 Protein (rp143202)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by anti-microbial activity against E.coli. is less than 30 μg/mL, corresponding to a specific activity of > 33.3 IU/mg.

SDS-PAGE: Recombinant Human Beta-Defensin 3 Protein (rp143202)

Recombinant Human Beta-Defensin 3 Protein (rp143202)-SDS-PAGE
Recombinant Human Beta-Defensin 3 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a single band at 5.2 kDa.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F0600351	分析证书	rp143202
ZJ23F0600352	分析证书	rp143202
ZJ23F0600350	分析证书	rp143202

引用文献

1. Harder, J J, Bartels, J J, Christophers, E E and Schroder, J M JM.. (2001) Isolation and characterization of human beta -defensin-3, a novel human inducible peptide antibiotic.. The Journal of biological chemistry, (23): [PMID:11085990]
2. Jia, H P HP and 10 more authors.. (2001) Discovery of new human beta-defensins using a genomics-based approach.. Gene, (24): [PMID:11223260]
3. Schibli, David J DJ and 7 more authors.. (2002) The solution structures of the human beta-defensins lead to a better understanding of the potent bactericidal activity of HBD3 against Staphylococcus aureus.. The Journal of biological chemistry, (8): [PMID:11741980]

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