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基本描述
| 英文名称 | RNA Sample Loading Buffer | ||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 储存温度 | -20°C储存 | ||||||||||||||||||||||||||
| 运输条件 | 超低温冰袋运输 | ||||||||||||||||||||||||||
| 产品介绍 |
RNA 上样缓冲液在 RNA 电泳期间用作追踪染料。这种染料带有轻微的负电荷，会向与 RNA 相同的方向迁移，使用户能够监测分子在凝胶中移动的进程。迁移速率因凝胶组成而异。用样品按 1:3 至 1:6 稀释，加热至 65℃，持续 10 分钟，上样前置于冰上冷却。 推荐用法：将 1 个体积的样品加入 2 至 5 倍的样品上样缓冲液中，混合均匀。将样品加热至 65°C，10 分钟，然后，在进行凝胶上样之前立刻在冰上冷却。 产品应用： RNA 样品上样缓冲液特别适用于在甲醛-琼脂糖凝胶（含有或不含溴化乙锭）上进行 RNA 电泳。在 Northern 印迹检测之前，不推荐使用溴化乙锭进行凝胶染色，因为琼脂糖凝胶或上样缓冲液中存在溴化乙锭会导致转移效率低下。适合与甲醛琼脂糖凝胶一起用于 Northern 印迹实验。 产品规格与参数：
Product introduction: RNA loading buffer is used as a tracking dye during RNA electrophoresis. This dye has a slight negative charge and will migrate in the same direction as RNA, enabling the user to monitor the progress of molecules moving in the gel. The migration rate varies with the composition of the gel. Dilute the sample in 1:3 to 1:6, heat it to 65 ℃ for 10 minutes, and cool it on ice before loading. Recommended usage: add 1 volume of sample into 2 to 5 times of sample loading buffer, and mix well. Heat the sample to 65 ° C for 10 minutes, and then cool it on ice immediately before gel loading. Product application: RNA sample loading buffer is especially suitable for RNA electrophoresis on formaldehyde agarose gel (with or without ethidium bromide). Before Northern blot detection, ethidium bromide is not recommended for gel staining, because ethidium bromide in agarose gel or loading buffer will lead to low transfer efficiency. It is suitable to be used with formaldehyde agarose gel for Northern blot experiments. Product specifications and parameters:
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质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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