磷酸酶抑制剂混合物（100×）

COA

级别和纯度:

BioReagent

用于蛋白分析

for western blot

100x

有货

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
P666104-1ml	1ml	现货

查看相关系列

WB (53) 抑制剂混合物 (13) 蛋白质提取 (8)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本描述

别名	磷酸酶抑制剂混合物液
英文别名	Phosphatase Inhibitor Cocktail
规格或纯度	BioReagent, for western blot, 用于蛋白分析, 100x
稳定性与储存	Store at -20℃ long term (12 months).
英文名称	Phosphatase Inhibitor Cocktail (100×)
储存温度	-20°C储存
运输条件	超低温冰袋运输
产品介绍	本品是一种广谱磷酸酶抑制剂，高效抑制Serine/Threonine Phosphatase，Tyrosine Phosphatase，Acid Phosphatase，Alkaline phosphatase等细胞内所含的磷酸酶。本产品含Sodium fluoride，Sodium orthovanadate，Sodium molybdate，Sodium tartrate， Imidazole，Sodium pyrophosphate decahydrate，β-Glycerophosphate，Cantharidin8种高效磷酸酶抑制剂，提供比同类产品（一般含3~4种抑制剂）更为全面的磷酸酶抑制能力。使用说明 1、本品用于各种蛋白质含量或其活性分析实验（Western Blot，Co-IP，pull-down，IF，IHC等），抑制磷酸酶的活性； 2、将本品以1:100（体积比）的稀释比加入到目标溶液中（如细胞裂解液，蛋白提取液等）。注意事项 1、当与蛋白酶抑制剂联用时，请不要使用含有EDTA的蛋白酶抑制剂，因为EDTA会严重影响磷酸酶抑制剂混合液中某些成分的抑制效果。事实上磷酸酶抑制剂混合液中已含有多种螯合剂成分； 2、本品为高浓度溶液，长期存放可能会出现微量晶体状沉淀，此为正常现象，不影响实验结果； 3、不同来源的样品中磷酸酶含量各不相同，1:100为推荐的常规剂量。实际使用中，如发现磷酸酶含量较高，可适当提高磷酸酶抑制剂混合液的使用浓度； 4、为了您的安全和健康，请穿实验服并戴一次性手套操作。所有抑制剂对人体均有毒，请勿直接与皮肤接触。如不小心接触皮肤，请立即用大量清水冲洗。如不慎吞食，应立即就医处理； 5、本产品仅限科研使用。 This product is a broad-spectrum phosphatase inhibitor that effectively suppresses various phosphatases present in cells, including Serine/Threonine Phosphatase, Tyrosine Phosphatase, Acid Phosphatase, and Alkaline Phosphatase. It contains 8 potent phosphatase inhibitors: 1. Sodium fluoride 2. Sodium orthovanadate 3. Sodium molybdate 4. Sodium tartrate 5. Imidazole 6. Sodium pyrophosphate decahydrate 7. β-Glycerophosphate 8. Cantharidin This comprehensive formulation provides superior phosphatase inhibition compared to conventional products (typically containing only 3–4 inhibitors). Protocol 1. Applicable to various protein quantification or activity analysis experiments (e.g., Western Blot, Co-IP, Pull-down, IF, IHC) to inhibit phosphatase activity. 2. Dilute the inhibitor cocktail 1:100 (v/v) into the target solution (e.g., cell lysis buffer, protein extraction buffer). Precautions 1. When used in combination with protease inhibitors, avoid protease inhibitors containing EDTA, as EDTA may significantly impair the efficacy of certain components in this phosphatase inhibitor cocktail. Note that this cocktail already contains multiple chelating agents. 2. This product is a high-concentration solution. Long-term storage may result in the formation of trace crystalline precipitate, which is normal and does not affect experimental results. 3. The recommended dilution ratio of 1:100 is suitable for general use. If the sample contains high levels of phosphatases, increase the concentration of the inhibitor cocktail accordingly. 4. For safety, wear a lab coat and disposable gloves during handling. All inhibitors are toxic to humans—avoid direct skin contact. In case of contact, rinse immediately with plenty of water. If ingested, seek medical attention immediately. 5. For research use only.