基本描述

产品名称

蛋白组学前处理试剂盒

规格或纯度

BioReagent, 无菌, 蛋白组学级

产品介绍

目前常规的蛋白质组样品前处理过程存在着诸多问题，如重现性差、方法稳定性不好、灵敏度不高且非常耗时及无法自动化等。针对上述问题，阿拉丁推出新一代“All In One Tube”的创新产品，本产品主要是针对微量蛋白（5-100μg）的处理，即便是没有组学背景的科研人员，也可以快速的制备前处理的样品，可用于后续的质谱分析。

蛋白组学前处理试剂盒（P1456469）实验流程图

产品组分及储存条件：

P1456469	Component	12 T	24T	48T	Storage
P1456469A	Lysis Buffer	1.25 mL	2.5 mL	5 mL	-20℃. Store in the dark.
P1456469B	Digest	0.05 mL	0.1 mL	0.2 mL	-20℃
P1456469C	Balance Buffer	3 mL	6 mL	12 mL	2-8℃
P1456469D	Stop Buffer	0.375 mL	0.75 mL	1.5 mL	RT
P1456469E	Wash Buffer I	5 mL	10 mL	20 mL	RT
P1456469F	Wash Buffer II	3.75 mL	7.5 mL	15 mL	RT
P1456469G	Wash Buffer III	3.75 mL	7.5 mL	15 mL	RT
P1456469H	Elution Buffer	6.25 mL	12.5 mL	25 mL	RT. Store in the dark.
P1456469I	Loading Buffer	0.25 mL	0.5 mL	1 mL	RT
P1456469J	Tip柱	12 T	24T	48T	RT

产品特点：

微量蛋白：针对5-100μg的蛋白前处理
操作简单：可快速制备前处理样品，仅需一台金属浴和常规离心机
稳定性高：各批次严格质检，实验结果重复性高

操作流程：

1.裂解

①取50μL的Lysis buffer加入到含有样品的EP管中，震荡混匀。

②将含有Lysis buffer的EP管水浴95°C、10min，然后取出冷却至室温。

2.酶解

①向冷却至室温的EP管中加入225μL的Balance buffer。

②加入3μL的Digest后混匀，37°C，1200rpm，震荡酶解，过夜(建议16h)。

3.除盐(室温)

①向样品中加入25μL的Stop buffer，涡旋混匀。

②再加入320μL Wash buffer 1,剧烈震荡3min，15000rpm离心3min，将上层液体去除。

③将下层样品转入Tip柱中，2500rpm/min,离心3-5min，直到液体全部离心下来，如果液体流速较慢，可以加大转速。

④向脱盐柱中加入200μL的Wash buffer 2 (用之前震荡10-20s)，2500rpm/min，离心3-5min，直到液体全部离心下来。

⑤向脱盐柱中加入200μL的Wash buffer 3，2500rpm/min，离心3-5min，直到液体全部离心下来。

⑥将脱盐柱重新放入一个新的EP管中，向脱盐柱中加入200μL的Elution buffer，2000rpm/min，离心3-5min，直到液体全部离心下来。

⑦重复步骤⑥，收集两次的洗脱液，冷冻干燥。

⑧加入10μL的Loading buffer，剧烈涡匀3min，离心20000g、10min，取适量样品，即可质谱检测。以HF-X仪器为例，上样0.5-1μg即可。

注意事项：

1. Digest分装后-20°C保存；

2. Lysis Buffer分装后-20°C保存，避免反复冻融；
3.本产品仅限于专业人员的科学研究使用，不得用于临床诊断或治疗，不得用于食品或药品。

Product Introduction:
At present, the conventional proteome sample pretreatment process has many problems, such as poor reproducibility, poor method stability, low sensitivity, being very time-consuming, and inability to be automated. To address the above issues, Aladdin has launched a new generation of "All In One Tube" innovative products. This product is mainly designed for the processing of trace proteins (5-100μg). Even researchers without omics background can quickly prepare pretreated samples, which can be used for subsequent mass spectrometry analysis.

Experimental Flowchart of Proteomics Pretreatment Kit (P1456469)

Product Components and Storage Conditions:

P1456469	Component	12 T	24T	48T	Storage
P1456469A	Lysis Buffer	1.25 mL	2.5 mL	5 mL	-20℃. Store in the dark.
P1456469B	Digest	0.05 mL	0.1 mL	0.2 mL	-20℃
P1456469C	Balance Buffer	3 mL	6 mL	12 mL	2-8℃
P1456469D	Stop Buffer	0.375 mL	0.75 mL	1.5 mL	RT
P1456469E	Wash Buffer I	5 mL	10 mL	20 mL	RT
P1456469F	Wash Buffer II	3.75 mL	7.5 mL	15 mL	RT
P1456469G	Wash Buffer III	3.75 mL	7.5 mL	15 mL	RT
P1456469H	Elution Buffer	6.25 mL	12.5 mL	25 mL	RT. Store in the dark.
P1456469I	Loading Buffer	0.25 mL	0.5 mL	1 mL	RT
P1456469J	Tip pillar	12 T	24T	48T	RT

Trace proteins: Designed for the pretreatment of 5-100μg proteins.
Simple operation: Enables rapid preparation of pretreated samples, requiring only a metal bath and a conventional centrifuge.
High stability: Strict quality inspection for each batch ensures high reproducibility of experimental results.

Operating Procedure:

Lysis
① Add 50μL of Lysis buffer to the EP tube containing the sample and mix by shaking.
② Place the EP tube with Lysis buffer in a 95°C water bath for 10 minutes, then take it out and cool to room temperature.
Enzymatic Digestion
① Add 225μL of Balance buffer to the EP tube that has cooled to room temperature.
② Add 3μL of Digest, mix well, and perform enzymatic digestion with shaking at 37°C and 1200rpm overnight (16 hours is recommended).
Desalting (at room temperature)
① Add 25μL of Stop buffer to the sample and vortex to mix.
② Add 320μL of Wash buffer 1, shake vigorously for 3 minutes, centrifuge at 15000rpm for 3 minutes, and remove the supernatant.
③ Transfer the lower layer sample into the Tip column, centrifuge at 2500rpm for 3-5 minutes until all liquid is centrifuged down. If the liquid flow rate is slow, the speed can be increased.
④ Add 200μL of Wash buffer 2 (shake for 10-20 seconds before use) to the desalting column, centrifuge at 2500rpm for 3-5 minutes until all liquid is centrifuged down.
⑤ Add 200μL of Wash buffer 3 to the desalting column, centrifuge at 2500rpm for 3-5 minutes until all liquid is centrifuged down.
⑥ Put the desalting column into a new EP tube, add 200μL of Elution buffer to the desalting column, centrifuge at 2000rpm for 3-5 minutes until all liquid is centrifuged down.
⑦ Repeat step ⑥, collect the eluates from both times, and freeze-dry them.
⑧ Add 10μL of Loading buffer, vortex vigorously for 3 minutes, centrifuge at 20000g for 10 minutes, take an appropriate amount of sample, and then mass spectrometry detection can be performed. Taking the HF-X instrument as an example, 0.5-1μg of sample is sufficient for loading.

Precautions:
After aliquoting, Digest should be stored at -20°C.
After aliquoting, Lysis Buffer should be stored at -20°C and avoid repeated freezing and thawing.
This product is limited to scientific research use by professionals, and must not be used for clinical diagnosis or treatment, nor for food or drugs.

储存与运输

物理形态	液体
储存温度	2-8°C储存,避光,室温,-20°C储存
运输条件	冰袋运输
稳定性与储存	各组分在相应的储存条件下保质期1年。
分子类型	试剂盒

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到2个结果

批号(Lot Number)	证书类型	货号
ZJ25F0927612	分析证书	P1456469
ZJ25F0926874	分析证书	P1456469

技术文档和文章

p1456469 蛋白组学前处理试剂盒中文说明书.pdf

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

=

浓度

x

体积

x

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

x

体积 1 (V1)

=

浓度 2 (C2)

x

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

=

质量（小瓶）

÷

所需的复溶浓度

货号 (SKU)	包装规格	是否现货
P1456469-12T	12T	现货
P1456469-24T	24T	现货
P1456469-48T	48T	期货

蛋白组学前处理试剂盒

库存信息

库存信息

库存信息

基本描述

储存与运输

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器