| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| O1501802-1ml |
1ml |
期货  |
| |
| O1501802-10×1ml |
10×1ml |
期货 |
|
| 规格或纯度 | 适用于免疫荧光(IF), BioReagent, 即用型, 生物染色剂, 适用于荧光分析, 生物染料级, 用于显微镜, 用于细胞培养, 1.0 mg/ml in H₂O |
|---|---|
| 稳定性与储存 | Store at -20℃ long term (12 months). Store in the dark. Avoid repeated freezing and thawing. |
| 英文名称 | oechst 33342 Staining Solution |
| 储存温度 | 避光,-20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 产品介绍 |
Hoechst 33342溶液是一种用于对活细胞或固定细胞中的DNA和细胞核进行荧光染色的试剂。Hoechst 33342是一种双苯并咪唑染料，对双链DNA具有高度特异性（更倾向于与A-T基对结合）。这种染料非常有助于标记双链DNA，从而实现对细胞核的可视化。Hoechst 33342可在约355 nm处被紫外线光源激发。当与DNA结合时，它会在461 nm的发射峰值附近发出蓝色荧光。 Hoechst33342常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。也可以用于普通的细胞核染色，或常规的DNA染色。 本产品浓度为1.0 mg/mL，用于细胞核染色时，推荐工作浓度为1-10 μg/mL。 使用方法 1.对于固定的细胞或组织 a. 将细胞接种于培养板/皿中，培养至对数生长期。如需固定细胞，用4%多聚甲醛室温固定10-15 min，PBS洗涤2次，每次5分钟。 b. 加入Hoechst 33342工作液，37°C避光孵育10-30 min。 c. 吸除Hoechst 33342染色液，用PBS洗涤2-3次，每次5 min。 2.对于活细胞或组织 a. 收集细胞，1000 rpm离心5 min，弃上清。 b. 用PBS重悬细胞，加入Hoechst 33342工作液，室温避光孵育15-20 min。 c. 1000 rpm离心5 min，弃染色液，用PBS洗涤2-3次；加入PBS重悬细胞。 d. 直接在荧光显微镜下观察或封片后荧光显微镜下观察。细胞发生凋亡时，会看到凋亡细胞的细胞核呈致密浓染，或呈碎块状致密浓染。 注意事项 1.根据Hoechst 33342染色液应用的不同，建议使用前进行滴定以确定最佳工作浓度。 2.荧光染料都存在淬灭的问题，建议染色后尽量当天完成检测。活细胞或组织染色后宜立即观察。 3.本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。 4.为了您的安全和健康，请穿实验服并戴一次性手套操作。
Hoechst 33342 solution is a reagent used for fluorescent staining of DNA and cell nuclei in living cells or fixed cells. Hoechst 33342 is a bis-benzimidazole dye with high specificity for double-stranded DNA (it is more inclined to bind to A-T base pairs). This dye is very helpful for marking double-stranded DNA, thereby enabling visualization of the cell nucleus. Hoechst 33342 can be excited by a UV light source at approximately 355 nm. When combined with DNA, it emits blue fluorescence at the emission peak of 461 nm. Hoechst 33342 is commonly used for cell apoptosis detection. After staining, it can be observed using a fluorescence microscope or detected by a flow cytometer. It can also be used for ordinary cell nucleus staining or routine DNA staining. The concentration of this product is 1.0 mg/mL. When used for cell nucleus staining, the recommended working concentration is 1-10 μg/mL. Instruction for use 1.For fixed cells or tissues: a. The cells were inoculated onto the culture plates/flasks and cultured until they reached the logarithmic growth phase. If cell fixation was required, the cells were fixed with 4% paraformaldehyde at room temperature for 10-15 minutes, then washed twice with PBS, each time for 5 minutes. b. Add the Hoechst 33342 working solution and incubate at 37°C in the dark for 10-30 minutes. c. Remove the Hoechst 33342 staining solution and wash the cells twice with PBS for 5 minutes each time. 2.For living cells or tissues： a. Collect the cells, centrifuge at 1000 rpm for 5 minutes, and discard the supernatant. b. Resuspend the cells in PBS, add the Hoechst 33342 working solution, and incubate at room temperature in the dark for 15-20 minutes. c. Centrifuge at 1000 rpm for 5 minutes, discard the staining solution, wash twice with PBS for 5 minutes each time; then resuspend the cells in PBS. d. Observe directly under a fluorescence microscope or under a fluorescence microscope after mounting. When cells undergo apoptosis, the nuclei of apoptotic cells will show dense and compact staining, or fragmented and dense staining. Matters needing attention 1.Depending on the application of Hoechst 33342 staining solution, it is recommended to perform a titration before use to determine the optimal working concentration. 2.Fluorescent dyes all have the problem of quenching. It is recommended to complete the detection as soon as possible after staining. Live cells or tissues should be observed immediately after staining. 3.This product is only for professional scientific research use. It shall not be used for clinical diagnosis or treatment, nor for food or medicine. It shall not be stored in ordinary residences. 4.For your safety and health, please wear laboratory coats and disposable gloves during operation.
|
| 分子类型 | 生物试剂/缓冲液 |
|---|---|
| Isomeric SMILES | CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C |
| 关联CAS | 875756-97-1 |
| 分子量 | 561.93（anhydrous basis） |
| Beilstein号 | 1234011 |
| Reaxy-Rn | 9095542 |
| Reaxys-RN link address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=9095542&ln= |
| 敏感性 | Light-sensitive |
|---|
| 象形图 | GHS08, GHS07 |
|---|---|
| 信号词 | Warning |
| 危险声明 |
H315: 引起皮肤刺激 H335: 可能引起呼吸道刺激 H341: 怀疑引起遗传缺陷 H302: 吞食有害 |
| 预防措施声明 |
P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾 P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。 P302+P352: 如皮肤沾染：用水充分清洗。 P321: 特殊处理（请参阅此标签上的...）。 P405: 密闭存放 P501: 将内容物/容器处理到。。。 P264: 处理后要彻底洗手。 P281: 根据需要使用个人防护设备。 P271: 仅在室外或通风良好的地方使用。 P270: 使用本产品时，请勿进食、饮水或吸烟。 P304+P340: 如误吸入：将人转移到空气新鲜处，保持呼吸舒适体位。 P403+P233: 存放在通风良好的地方。保持容器密闭。 P362+P364: 脱掉沾污的衣服，清洗后方可重新使用。 P330: 漱口 P203: 使用前，获取、阅读并遵守所有安全说明。 P301+P317: 如果被吞咽：请寻求医疗帮助。 P318: 如果暴露或担心，请就医。 P332+P317: 如果出现皮肤刺激：请寻求医疗帮助。 P319: 如果你感到不适，请寻求医疗帮助。 |
| WGK Germany | 3 |
| 应用名称 | 稀释比例 |
|---|---|
| Flow Cytometry |
1-10 μg/ml
|
首页
400-620-6333
