计算溶液所需的质量、体积或浓度。
This is a demo store. No orders will be fulfilled.
| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| N752097-2×1ml |
2x1ml |
现货 ![]() |
| |
| N752097-10×1ml |
10×1ml |
现货 ![]() |
|
| 别名 | 非变性PAGE蛋白上样缓冲液 | 非变性还原PAGE蛋白上样缓冲液 | 非变性PAGE蛋白上样缓冲液(5 x) | 非变性还原PAGE蛋白上样缓冲液(5 x) |
|---|---|
| 英文别名 | Native Gel Sample Loading Buffer (5X) |
| 规格或纯度 | BioReagent, for western blot, 用于聚丙烯酰胺凝胶电泳, 5X |
| 稳定性与储存 | Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. |
| 英文名称 | Native-PAGE Loading Buffer (5X) |
| 储存温度 | 避光,-20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 产品介绍 |
非变性蛋白上样缓冲液 (5×) 是以溴酚蓝为染料,5倍浓缩的蛋白上样缓冲液,具有沉淀蛋白样品进入凝胶孔和指示蛋白样品在凝胶中运动位置的作用,缓冲液中不含有 SDS 等变性剂,但是含有 DTT/β-巯基乙醇等还原剂,适用于非变性还原型的蛋白样品制备和电泳。 1.1 将非变性蛋白上样缓冲液(5×) 与蛋白样品按照 1:4 的比例混匀; 2、蛋白样品处理 2.1 蛋白样品以小于3000rpm的条件离心30秒; 3、蛋白上样及电泳 3.1 离心后,以微量进样器取适量上清,直接加入凝胶加样孔内; 3.2 进行常规电泳,通常染料到达距离凝胶底端0.5cm-1cm处即可停止电泳。 Native-PAGE Loading Buffer (reduced, 5X) is a 5-fold concentrated SDS-PAGE gel electrophoresis loading buffer with bromophenol blue as the dye. It has the function of precipitating protein samples into gel pores and indicating the movement position of protein samples in gel. The buffer does not contain denaturants such as SDS, but contains reducing agents such as DTT/β - mercaptoethanol. It is suitable for the preparation and electrophoresis of non denatured reduced protein samples. 1. Before adding the sample, thaw it at room temperature or 37-40 ℃ for a few minutes and gently shake well to ensure even mixing of the solution; 1. Reagent preparation |
| pH | 6.2-7.2 |
|---|
| 分子类型 | 生物试剂/缓冲液 |
|---|
| 敏感性 | Light sensitive |
|---|
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | N752097 | |
| 分析证书 | N752097 |
¥54.90
¥101.90
¥83.90
¥58.90