基本描述

英文名称

NGS Barcode Adapters 1-16 Kit for Ion Torrent

储存温度

-20°C储存,避免反复冻融

运输条件

超低温冰袋运输

产品介绍

产品内容

N670210	Component	16×10 μL	Storage
N670210A	Ion P1 Adapter (10 μM)	160 μL	-20℃. Avoid freeze/thaw cycle.
N670210B	Ion Barcode Ax (10 μM)	16×10 μL	-20℃. Avoid freeze/thaw cycle.

产品简介

NGS Barcode Adapters for Ion Torrent (1-16)是针对Ion Torrent高通量测序平台文库构建的专用配套试剂盒，可用于构建适用于Ion PGM™ System、Ion Proton™ System、Ion S5™ System和Ion GeneStudio™ S5 Series System等Ion Torrent高通量测序平台的多样品靶向测序DNA文库。本试剂盒包含Ion Torrent测序平台文库建库中使用的P1 Adaptor与Barcode A1至Barcode A16共计16种不同Barcode接头，与测序快速DNA建库试剂盒和AmpliSeq二代测序DNA文库构建试剂盒配套使用，可用于构建16种index标记DNA文库进行测序分析。试剂盒中提供的所有试剂都经过了严格的质量控制和功能验证，最大程度上保证了样品识别的可靠性、文库构建的稳定性和重复性。

注意事项

1．Adapter为双链结构，请勿将其置于室温温度以上，以免发生解链，影响使用。

2．Barcode Adapter开盖前请短暂离心，使液体收集到管底，以免不同Barcode Adapter 交叉污染。

操作步骤

本试剂盒中每种Barcode Adapter包装量足够进行≤14个100 ng Input DNA或者1个 1 µg Input DNA的文库构建。接头使用量可根据样本DNA起始量进行调整，以达到最优使用效果。

以下操作示例按照测序快速DNA建库试剂盒进行：

1. 在一个新的0.2 ml PCR管中依次加入以下试剂并充分混匀。

Component	Volume	Volume
	50-100-ng DNA Input	1-µg DNA Input
End Repaired DNA Fragment	60 μl	60 μl
Ligation and Nick Repair Buffer	10 μl	10 μl
Ion P1 Adapter	7 μl(1 μM )	7 μl(10 μM )
Ion Barcode Ax	7 μl(1 μM )	7 μl(10 μM )
Nuclease-free Water	12 μl	10 μl
DNA Ligase	2 μl	4 μl
Bst DNA Polymerase	2 μl	2 μl
Total volume	100 μl	100 μl

注意：建议Adaptor的加入量与DNA片段的摩尔比为10:1-20:1，具体Adaptor的使用浓度和使用量请参照测序快速DNA建库试剂盒使用说明书进行：DNA量为10-100 ng 时，Adaptor建议使用浓度为1 μM（小于260 bp）或0.5 μM（300-400 bp）；DNA量为1 µg时，Adaptor 建议使用浓度为10 μM（小于260 bp）或 5 μM（300-400 bp）。
2. 将PCR管置于PCR仪中，热盖设置为80℃，运行如下程序：

Stage	Temperature	Time
Hold	25℃	15 minutes
Hold	65℃	5 minutes
Hold	4℃	Hold

注意：连接接头后请尽快进行下一步操作，进行接头连接产物的纯化。

序列信息
Ion P1 Adapter：5’-CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGAT-3’ Ion Barcode Ax：5’-CCATCTCATCCCTGCGTGTCTCCGACTCAGXXXXXXXXXXGAT-3’ *下划线标记序列X即为测序时的barcode序列。
各Barcode Adapter对应的barcode的序列如下：

Component	Barcode
Ion Barcode A1	CTAAGGTAAC
Ion Barcode A2	TAAGGAGAAC
Ion Barcode A3	AAGAGGATTC
Ion Barcode A4	TACCAAGATC
Ion Barcode A5	CAGAAGGAAC
Ion Barcode A6	CTGCAAGTTC
Ion Barcode A7	TTCGTGATTC
Ion Barcode A8	TTCCGATAAC
Ion Barcode A9	TGAGCGGAAC
Ion Barcode A10	CTGACCGAAC
Ion Barcode A11	TCCTCGAATC
Ion Barcode A12	TAGGTGGTTC
Ion Barcode A13	TCTAACGGAC
Ion Barcode A14	TTGGAGTGTC
Ion Barcode A15	TCTAGAGGTC
Ion Barcode A16	TCTGGATGAC

Product content:

N670210	Component	16×10 μL	Storage
N670210A	Ion P1 Adapter (10 μM)	160 μL	-20℃. Avoid freeze/thaw cycle.
N670210B	Ion Barcode Ax (10 μM)	16×10 μL	-20℃. Avoid freeze/thaw cycle.

Product Introduction:

NGS Barcode Adapter for Ion Torrent (1-16) is a specialized kit designed for library construction on Ion Torrent high-throughput sequencing platforms. It can be used to construct libraries suitable for Ion PGM ™ System, Ion Proton System, Ion S5 System, and Ion GeneStudio ™ Multi sample targeted sequencing DNA library using Ion Torrent high-throughput sequencing platforms such as S5 Series System. This kit contains 16 different barcode adapters, including P1 Adaptor and Barcode A1 to Barcode A16, used in the library construction of the Ion Torrent sequencing platform. It is compatible with the Kangwei Century second-generation sequencing rapid DNA library construction kit and the AmpliSeq second-generation sequencing DNA library construction kit and can be used to construct 16 index labeled DNA libraries for sequencing analysis. All reagents provided in the kit have undergone strict quality control and functional validation, ensuring the reliability of sample identification, stability and repeatability of library construction to the greatest extent possible.

Notice:

1. The adapter is a double chain structure, please do not place it above room temperature to avoid chain breakage and affect use.

2. Before opening the barcode adapter, please centrifuge briefly to collect the liquid at the bottom of the tube to avoid cross contamination between different barcode adapters.

Operation steps
The packaging quantity of each Barcode Adapter in this kit is sufficient for library construction of ≤ 14 pieces of 100 ng Input DNA or 1 piece of 1 µ g Input DNA. The amount of adapter used can be adjusted according to the initial amount of DNA in the sample to achieve optimal usage effect. The following operation example is performed using the Kangwei Century second-generation sequencing rapid DNA library kit:

1. Add the following reagents sequentially into a new 0.2 ml PCR tube and mix thoroughly.

Component	Volume	Volume
	50-100-ng DNA Input	1-µg DNA Input
End Repaired DNA Fragment	60 μl	60 μl
Ligation and Nick Repair Buffer	10 μl	10 μl
Ion P1 Adapter	7 μl(1 μM )	7 μl(10 μM )
Ion Barcode Ax	7 μl(1 μM )	7 μl(10 μM )
Nuclease-free Water	12 μl	10 μl
DNA Ligase	2 μl	4 μl
Bst DNA Polymerase	2 μl	2 μl
Total volume	100 μl	100 μl

Attention: It is recommended that the molar ratio of the amount of Adaptor added to the DNA fragment be 10:1-20:1. Please refer to the instructions for using the Kangwei Century second-generation sequencing rapid DNA library kit for specific concentrations and amounts of Adaptor. When the DNA amount is 10-100 ng, the recommended concentration of Adaptor is 1 μ M (less than 260 bp) or 0.5 μ M (300-400 bp); When the DNA content is 1 µ g, Adaptor recommends using a concentration of 10 μ M (less than 260 bp) or 5 μ M (300-400 bp).
2. Place the PCR tube in the PCR instrument, set the hot cap to 80 ℃, and run the following program:

Stage	Temperature	Time
Hold	25℃	15 minutes
Hold	65℃	5 minutes
Hold	4℃	Hold

Attention: Please proceed to the next step as soon as possible after connecting the connector to purify the product of the connector connection.

Sequence information
Ion P1 Adapter：5’-CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGAT-3’ Ion Barcode Ax：5’-CCATCTCATCCCTGCGTGTCTCCGACTCAGXXXXXXXXXXGAT-3’ *The underlined sequence X is the barcode sequence used during sequencing.
The sequence of barcodes corresponding to each Barcode Adapter is as follows:

Component	Barcode
Ion Barcode A1	CTAAGGTAAC
Ion Barcode A2	TAAGGAGAAC
Ion Barcode A3	AAGAGGATTC
Ion Barcode A4	TACCAAGATC
Ion Barcode A5	CAGAAGGAAC
Ion Barcode A6	CTGCAAGTTC
Ion Barcode A7	TTCGTGATTC
Ion Barcode A8	TTCCGATAAC
Ion Barcode A9	TGAGCGGAAC
Ion Barcode A10	CTGACCGAAC
Ion Barcode A11	TCCTCGAATC
Ion Barcode A12	TAGGTGGTTC
Ion Barcode A13	TCTAACGGAC
Ion Barcode A14	TTGGAGTGTC
Ion Barcode A15	TCTAGAGGTC
Ion Barcode A16	TCTGGATGAC

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

技术文档和文章

ngs barcode adapters 1-16 kit for ion torrent.pdf

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分子量

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浓度1（C1）

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浓度 2 (C2)

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复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

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质量（小瓶）

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所需的复溶浓度

二代测序接头引物试剂盒(1-16)

库存信息