基本描述

别名	核酸绿
英文别名	NA-Green \| Nucleic Acid Green \| Gel-Green \| Gel-Green DNA Stain
规格或纯度	≥90%(HPCE), 10000 x in DMSO
稳定性与储存	长期储存2-8℃（2年）。
英文名称	Gelgreen Nucleic Acid Gel Dyes (Aggregation-induced emission, AIE)
储存温度	2-8°C储存
运输条件	冰袋运输
产品介绍	储存缓冲液：DMSO AIE-Gelgreen 核酸凝胶染料是一种基于聚集诱导发光荧光材料的四苯乙烯衍生物，具有典型的AIE特性。产品可以作为各种核酸电泳的染色剂，适用于各种片段大小染色。与标准凝胶成像系统和可见光激发的凝胶观察装置兼容，适用于紫外凝胶成像系统或蓝色可见光激发的凝胶观察装置，特别是蓝色可见光激发的凝胶观察装置。 AIE-Gelgreen 核酸凝胶染料是一种灵敏度高、稳定性强、适用性广且极具兼容性的核酸凝胶染料，可用于对琼脂糖凝胶和聚丙烯酰胺凝胶中的dsDNA，ssDNA以及RNA进行染色。其表现出了极高的灵敏度，检测限均可低至pg级别。在胶染方面，是银染的理想替代品，在5min内即可获得清晰明亮的条带，操作简单，结果直观。并且 AIE-Gelgreen 核酸凝胶染料具有较宽的激发光谱特性，因此极具兼容性，可以在不改变任何成像系统的情况下用来替换溴化乙锭（EB）和其它凝胶染料，适用于原先使用 SYBR Green 或 SYBR Gold 等为核酸染料的凝胶成像和检测系统，这可使其成为与激光扫描仪配合使用的理想选择。并且，其可以使用对人体无害的蓝光灯或蓝光成像仪进行核酸检测，从而避免常规的紫外检测对核酸样品的致突变性，以及紫外对人的眼睛和皮肤的伤害。产品优势（1）灵敏度高：适用于各种大小片段的核酸电泳染色，灵敏度高至pg级别；（2）稳定性强：可使用微波炉加热，室温保存2年效果无影响；（3）兼容性广：通道匹配标准凝胶成像系统以及可见光激发的凝胶观察装置；（4）快速简单：泡染5min内即可获得清晰明亮的条带，无需脱色或冲洗；（5）操作性强：在0.1×至1×的浓度范围内，均具有较高的信噪比；（6）适用性广：适用于琼脂糖凝胶或聚丙烯酰胺凝胶电泳，可对dsDNA、ssDNA或RNA染色；实验方法一、胶染法（前染法）（用法同EB，推荐） 1．按常规操作，制备琼脂糖凝胶，加入浓缩的 AIE-Gelgreen 核酸凝胶染料，使其在凝胶中的终浓度为1×（比如，制备100mL凝胶，加入染料10μL，可根据实际情况调整用量），轻轻摇匀，倒胶。 2．按常规方法电泳，观测结果。二、点染法 1．按常规操作，制备琼脂糖凝胶，待胶凝固后，将染料与样品及loading buffer进行混匀，后加载到上样孔中（不影响迁移速率）。 2．按常规方法电泳，观测结果。三、泡染法（后染法） 1.按照常规方法进行电泳。 2.用ddH2O将AIE-Gelgreen 核酸凝胶染料浓缩液稀释约2500倍到0.1M的NaCl中，制成2×染色液。（比如，将15-20μL10000× AIE-Gelgreen 核酸凝胶染料浓缩液和5mL 1M NaCl加到45mL ddH2O中）。 3.将凝胶小心放入合适的容器中，缓慢加入足量的2×染色液浸没胶。室温振荡染色约5min即可，最佳染色时间根据凝胶厚度及琼脂糖浓度不同而略有不同。对于3.5-10%丙烯酰胺胶，染色时间可以适当延长。然后观测结果。注意事项 1、使用前请先短暂混匀； 2、为了您的安全和健康，请穿实验服并戴一次性手套操作； 3、本品仅适用于科研用途。 AIE-Gelgreen Nucleic Acid Gel Dyes is a tetraphenylethylene derivative based on aggregation-induced emission (AIE) fluorescent materials, with typical AIE properties. This product can be used as a staining agent for various nucleic acid electrophoresis, suitable for staining nucleic acid fragments of different sizes. It is compatible with standard gel imaging systems and gel observation devices excited by visible light, especially those excited by blue visible light. AIE-Gelgreen Nucleic Acid Gel Dyes is a highly sensitive, stable, versatile and highly compatible nucleic acid gel dye, which can be used to stain dsDNA, ssDNA and RNA in agarose gels and polyacrylamide gels. It exhibits extremely high sensitivity, with a detection limit as low as the pg level. In terms of gel staining, it is an ideal alternative to silver staining. Clear and bright bands can be obtained within 5 minutes, with simple operation and intuitive results. Moreover, AIE-Gelgreen nucleic acid gel dye has a wide excitation spectrum, thus featuring strong compatibility. It can replace ethidium bromide (EB) and other gel dyes without changing any imaging system, and is suitable for gel imaging and detection systems that originally use SYBR Green or SYBR Gold as nucleic acid dyes, making it an ideal choice for use with laser scanners. In addition, it allows nucleic acid detection using blue light lamp or blue light imagers that are harmless to the human body, thereby avoiding the mutagenicity of conventional ultraviolet detection to nucleic acid samples and the damage of ultraviolet rays to human eyes and skin. Product Advantages (1) High sensitivity: Suitable for staining nucleic acid electrophoresis of fragments of various sizes, with a sensitivity as high as the pg level; (2) Strong stability: Can be heated in a microwave oven, and stored at room temperature for 2 years without affecting its effectiveness; (3) Wide compatibility: The channel is compatible with standard gel imaging systems and gel observation devices excited by visible light; (4) Fast and simple: Clear and bright bands can be obtained within 5 minutes of soaking staining, without the need for decolorization or rinsing; (5) Strong operability: It has a high signal-to-noise ratio within the concentration range of 0.1× to 1×; (6) Wide applicability: Suitable for agarose gel or polyacrylamide gel electrophoresis, and can stain dsDNA, ssDNA or RNA; Experimental Methods I. Gel staining method (pre-staining method) (used the same as EB, recommended) 1.Prepare agarose gel according to routine operations, add concentrated AIE-Gelgreen Nucleic Acid Gel Dyes to make its final concentration in the gel 1× (for example, to prepare 100mL gel, add 10μL of dye; the dosage can be adjusted according to actual conditions), shake gently, and pour the gel. 2.Perform electrophoresis according to routine methods and observe the results. II. Spot staining method 1.Prepare agarose gel according to routine operations. After the gel solidifies, mix the dye with the sample and loading buffer, then load it into the sample well (does not affect the migration rate). 2.Perform electrophoresis according to routine methods and observe the results. III. Soaking staining method (post-staining method) 1.Perform electrophoresis according to routine methods. 2.Dilute the AIE-Gelgreen Nucleic Acid Gel Dyes concentrate about 2500 times with ddH2O into 0.1M NaCl to prepare 2× staining solution. (For example, add 15-20μL of 10000× AIE-Gelgreen Nucleic Acid Gel Dyes concentrate and 5mL of 1M NaCl to 45mL of ddH2O). 3.Carefully put the gel into a suitable container, slowly add enough 2× staining solution to submerge the gel. Stain with shaking at room temperature for about 5 minutes. The optimal staining time varies slightly depending on the gel thickness and agarose concentration. For 3.5-10% acrylamide gels, the staining time can be appropriately extended. Then observe the results. Precautions 1.Please mix briefly before use; 2.For your safety and health, please wear a lab coat and disposable gloves during operation; 3.This product is only for scientific research purposes.
纯度	≥90%(HPCE)

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批号(Lot Number)	证书类型	货号
ZJ25F0927194	分析证书	N1375492
ZJ25F0927193	分析证书	N1375492
ZJ25F0927192	分析证书	N1375492

技术文档和文章

n1375492_gelgreen 核酸凝胶染料聚集诱导发光产品说明书.pdf

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货号 (SKU)	包装规格	是否现货
N1375492-0.2ml	0.2ml	现货
N1375492-1ml	1ml	现货
N1375492-5ml	5ml	现货

Gelgreen 核酸凝胶染料（聚集诱导发光）

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基本描述

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器