基本描述

规格或纯度

for NGS Size Selection

英文名称

MagBead DNA Purification Kit

储存温度

2-8°C储存

运输条件

冰袋运输

产品介绍

产品内容：

Component	M666134 5 ml	M666134 50 ml
CMPure	5 ml	50 ml

产品简介

本试剂盒提供了一种简单、快速、高效的核酸纯化方法。该产品可用于二代测序建库时DNA的选择性或非选择性回收，以及PCR产物的纯化回收。 CMPure与样品按一定比例混合后，磁珠选择性将核酸吸附。经两步漂洗后，洗脱得到的DNA纯度高， A260/A280 的比值在 1.7-1.9之间, A260/A230 的比值通常在2.0以上。经该试剂盒纯化得到的 DNA适用于PCR，Real-Time PCR，测序， southern blotting等实验。

试剂盒说明

Sample type	Typical yield	Sample type	Typical yield
5000 bp segment	Up to 90%	1000 bp segment	Up to 90%
500 bp segment	Up to 80%	200 bp segment	Up to 70%

自备仪器、试剂

1.磁力架

2.80%乙醇。

3.洗脱液： Buffer EB (10 mM Tris-HCl, pH 8.0)；去离子水 (pH在7.0-8.0之间)。

实验前准备及重要注意事项

1.冰冻、离心、超声会对CMPure中的磁珠造成不可逆的损害。

2.CMPure中磁珠长期放置后会聚集成团，从而使磁珠表面积减小，降低样品回收得率，使用前一定要涡旋振荡彻底混匀磁珠。

3.使用前，建议将CMPure涡旋震荡混匀后分装到1.5 ml的离心管中，每管分装1 ml CMPure。

4.本试剂盒不适用于纯化回收小于100 bp的DNA片段，如果要回收小于100 bp的DNA 片段，建议将CMPure的用量增加到样品体积的4倍。

5.进行DNA的选择性回收时， CMPure对于DNA溶液中的离子浓度较为敏感。不同厂家的二代测序建库试剂盒得到的接头连接后的DNA溶液以及PCR扩增产物中离子浓度不同，所以用CMPure做DNA选择性回收时,试剂用量有所不同。

操作步骤

1.涡旋振荡CMPure 20秒，使其彻底混匀为均一溶液。

2.向1.5 ml的离心管中加入纯化的DNA溶液。

3.向上一步的离心管中加入2倍样品体积的CMPure，涡旋震荡5秒后室温静置5分钟。

4.将上一步的离心管放于磁力架上，直至磁珠完全吸附（约需5分钟）。

5.保持离心管固定于磁力架上，彻底弃去溶液，期间避免接触磁珠。

6.继续保持离心管固定于磁力架上，向离心管中加入250 µl新鲜配置的80%乙醇。

7.保持离心管固定于磁力架上，待悬起的磁珠完全吸附后彻底弃去乙醇。

8.重复步骤6-7两次。

9.保持离心管固定于磁力架上静置放置10分钟，使乙醇完全挥发干净。

10.将离心管从磁力架上取下，加入20-100 µl EB（自备）或去离子水，涡旋振荡使磁珠完全重悬于洗脱液中后，室温放置5分钟。

11.将离心管放于磁力架上直至磁珠完全吸附（约需5分钟）。

12.将洗脱液转移至一个新的1.5 ml离心管中。此时，可弃去磁珠。

纯化回收得率的计算

我们建议通过琼脂糖电泳纯化前后的样品进行回收得率的计算。我们不建议通过 260 nm处的光吸收值来计算回收得率。因为溶液中单链、双链DNA和dNTP以及一些纯化前的某些杂质在260 nm处都有光吸收，这样在计算回收前样品中DNA浓度时会得到一个假的、虚高的DNA浓度。

Product content:

Component	M666134 5 ml	M666134 50 ml
CMPure	5 ml	50 ml

Product Introduction
This reagent kit provides a simple, fast, and efficient method for nucleic acid purification. This product can be used for selective or non selective recovery of DNA during second-generation sequencing library construction, as well as purification and recovery of PCR products. After mixing CMPure with the sample in a certain proportion, magnetic beads selectively adsorb nucleic acids. After two steps of rinsing, the purified DNA obtained is high, with a ratio of A260/A280 between 1.7-1.9 and a ratio of A260/A230 typically above 2.0. The DNA purified by this reagent kit is suitable for PCR, Real Time PCR, sequencing, southern blotting, and other experiments.

Kit Description

Sample type	Typical yield	Sample type	Typical yield
5000 bp segment	Up to 90%	1000 bp segment	Up to 90%
500 bp segment	Up to 80%	200 bp segment	Up to 70%

Self provided instruments and reagents
1. Magnetic frame
2.80% ethanol.
3. Elution solution: Buffer EB (10 mM Tris HCl, pH 8.0); Deionized water (pH between 7.0 and 8.0).

Preparation and important precautions before the experiment
1. Freezing, centrifugation, and ultrasound can cause irreversible damage to the magnetic beads in CMPure.
2.After long-term storage, the magnetic beads in CMPure will aggregate into clusters, reducing the surface area of the magnetic beads and reducing the sample recovery rate. Before use, it is necessary to thoroughly mix the magnetic beads with vortex oscillation.
3. Before use, it is recommended to mix CMPure with vortex shaking and divide it into 1.5 ml centrifuge tubes, with 1 ml of CMPure per tube.
4. This reagent kit is not suitable for purifying and recovering DNA fragments less than 100 bp. If you want to recover DNA fragments less than 100 bp, it is recommended to increase the amount of CMPure to four times the sample volume.
5.When selectively recovering DNA, CMPure is more sensitive to the ion concentration in the DNA solution. The concentration of ions in the DNA solution and PCR amplification products obtained from the second generation sequencing library kits of different manufacturers varies after connector connection. Therefore, when using CMPure for DNA selective recovery, the amount of reagents used varies.

Operation steps
1. Vortex oscillate CMPure for 20 seconds to thoroughly mix into a homogeneous solution.
2. Add purified DNA solution to a 1.5 ml centrifuge tube.
3. Add twice the sample volume of CMPure to the centrifuge tube in the previous step, vortex for 5 seconds, and let it stand at room temperature for 5 minutes.
4. Place the centrifuge tube from the previous step on the magnetic stand until the magnetic beads are completely adsorbed (about 5 minutes).
5. Keep the centrifuge tube fixed on the magnetic frame, completely discard the solution, and avoid contact with the magnetic beads during this process.
6. Continue to keep the centrifuge tube fixed on the magnetic frame and add 250 µ l of freshly prepared 80% ethanol to the centrifuge tube.
7. Keep the centrifuge tube fixed on the magnetic frame, and completely discard the ethanol after the suspended magnetic beads are fully adsorbed.
8. Repeat steps 6-7 twice.
9. Keep the centrifuge tube fixed on the magnetic frame and let it stand for 10 minutes to completely evaporate the ethanol.
10. Remove the centrifuge tube from the magnetic rack, add 20-100 µ l of EB (self prepared) or deionized water, vortex oscillate to completely resuspend the magnetic beads in the eluent, and leave at room temperature for 5 minutes.
11. Place the centrifuge tube on the magnetic frame until the magnetic beads are completely adsorbed (about 5 minutes).
12. Transfer the eluent to a new 1.5 ml centrifuge tube. At this point, the magnetic beads can be discarded.
Calculation of purification recovery rate
We suggest calculating the recovery rate of samples before and after purification by agarose electrophoresis. We do not recommend calculating the recovery rate based on the light absorption value at 260 nm. Because single stranded, double stranded DNA, dNTP, and some impurities before purification in the solution have light absorption at 260 nm, a false and artificially high DNA concentration will be obtained when calculating the DNA concentration in the sample before recovery.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

技术文档和文章

m666134_magbead dna purification kit.pdf

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

=

浓度

x

体积

x

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

x

体积 1 (V1)

=

浓度 2 (C2)

x

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

=

质量（小瓶）

÷

所需的复溶浓度

货号 (SKU)	包装规格	是否现货	价格	数量
M666134-5ml	5ml	期货
M666134-50ml	50ml	期货

磁珠法DNA纯化回收试剂盒

库存信息