基本描述

规格或纯度	BioReagent, 用于细胞培养, sterile-filtered
英文名称	MEM Vitamin Solution (100×)
储存温度	避光,-20°C储存
运输条件	超低温冰袋运输
产品介绍	MEM维生素溶液(100×)是一种适合细胞培养的无菌过滤生物试剂。目前使用的合成/基础培养基含有氨基酸、碳水化合物、维生素和盐。Eagle′最低基础培养基(MEM)′是BME的改进版，其每种氨基酸含量都有所增加。为各种培养基添加水溶性维生素（如肌醇），以促进仓鼠和兔囊胚的孵化。此外，维生素还会影响绵羊和小鼠胚胎中葡萄糖的代谢。用途： MEM 维生素溶液 (100×) 用于以下用途：在配制 DMEM 培养基（用于原代神经细胞培养）用于维持伊格尔氏基本成分培养基中的细胞系（培养基中添加了 MEM 维生素溶液及其他成分）向培养基中添加维生素补充剂可刺激生长并延长培养细胞的活力。这种浓缩补充剂增加了营养物质，减少了细胞在体外的生物合成负担。 MEM维生素溶液(100×)用于：神经元原代培养的胶质条件培养基 in LB培养基或基本培养基(MM) Aa剥夺实验的私人培养基 PH：5.4-7.7 溶剂：0.85% NaCl 注意事项： 1. 本产品经0.1 μm过滤除菌； 2. 使用本产品时应注意无菌操作，避免污染； 3. 本产品为100×浓缩液，请根据需要稀释后使用； 4. 不宜长时间放置于室温环境或2-8℃长期保存； 5. 2-8℃中解冻，摇匀后使用，切忌反复冻融，用量较少时建议分装冻存； 6. 本产品仅用于科研或进一步研究使用，不用于诊断和治疗。 MEM Vitamin Solution (100×) is a sterile-filtered bioreagent, which is suitable for cell culture. Synthetic/ basal media used currently contain amino acids, carbohydrates, vitamins and salts. Eagle′s minimum essential medium (MEM) is a modified version of BME (basal medium Eagle′s), which contains increased amounts of each amino acid. Addition of water soluble vitamins such as, inositol to various culture facilitates the hatching of hamster and rabbit blastocysts. Vitamins also influence the metabolism of glucose in sheep and mouse embryos. Purpose： MEM Vitamin Solution (100×) has been used in: glial conditioned medium for primary neuronal culture in Luria-Bertani (LB) medium or minimal medium (MM) in private medium (PM = aminoacid-starved medium) for the Aa deprivation experiments MEM Vitamin Solution (100×) is used for the following applications: In the preparation of DMEM medium (for primary neural cell culture) For the maintenance of cell lines in Eagle′s Minimum Essential Medium (the medium is supplemented with MEM Vitamin Solution along with other components)

名称和识别符

分子类型	未知

化学和物理性质

敏感性	对光敏感

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批号(Lot Number)	证书类型	货号
I2529442	分析证书	M485434

技术文档和文章

常用细胞培养基指南

Categories: 技术文章

探索主题:

生物缓冲液生长因子细胞培养基原料细胞培养添加物氨基酸维生素盐葡萄糖核苷及核苷酸碳水化合物细胞培养和细胞转染

引用文献

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MEM 维生素溶液 (100×)

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