LV-320

COA

级别和纯度:

≥95%

CAS编号: 2449093-46-1
分子式: C₂₉H₂₆ClNO₂S₂
分子量: 520.11
PubChem编号: 134817239

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货号 (SKU)	包装规格	是否现货
L648052-5mg	5mg	期货
L648052-10mg	10mg	期货
L648052-50mg	50mg	期货

查看相关系列

Amino Acid/Protein Metabolism (4559) Atg and Atg-related Protein (255) Autophagy (2848) Cathepsin (64) Metabolic Enzyme/Protease (4889)

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基本描述

规格或纯度	≥95%
英文名称	LV-320
生化机理	LV-320 是一种强效、非竞争性 ATG4B 抑制剂，其 IC 50 为 24.5u2009µM，K d 为 16u2009µM。LV-320 可抑制 ATG4B 酶的活性，阻断细胞中的自噬通量，并且稳定、无毒，在体内具有活性。
储存温度	-20°C储存
运输条件	超低温冰袋运输
产品介绍	LV-320 is a potent and uncompetitive ATG4B inhibitor with an IC 50 of 24.5 µM and a K d of 16 µM. LV-320 inhibits ATG4B enzymatic activity, blocks autophagic flux in cells, and is stable, non-toxic and active in vivo In Vitro LV-320 (0-120 µM; SKBR3, MCF7, JIMT1, and MDA-MB-231 cells) treatment results in a dose-dependent increase in endogenous LC3B-II and protein p62 levels in all four cell lines. LV-320 (120 µM; 48 hours; MDA-MB-231 cells) treatment results in an increase in LC3B-II, indicating that LV-320 blocks autophagic flux. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: SKBR3, MCF7, JIMT1, and MDA-MB-231 cells Concentration: 0 µM, 25 µM, 50 µM, 75 µM, 100 µM, or 120 µM Incubation Time: Result: Resulted in a dose-dependent increase in endogenous LC3B-II and protein p62 levels in all four cell lines. Cell Autophagy AssayCell Line: MDA-MB-231 cells Concentration: 120 µM Incubation Time: 48 hours Result: Blocked autophagic flux. In Vivo LV-320 (100-200 mg/kg; oral gavage; three times over two days; GFP-LC3 mice) treatment results in a terminal blood level of 169 µM and a liver level of 104 µM. The expression of GFP-LC3 puncta is significantly greater accumulation in LV-320 treated animals compared to controls. LC3B-II protein is also increased in LV-320-treated animals. The treatment do not cause significant toxicity in mice at either dose . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: GFP-LC3 mice (females, 9-14 weeks) Dosage: 100 mg/kg or 200 mg/kg Administration: Oral gavage; three times over two days (Pharmacokinetic study) Result: Terminal blood levels were 169 µM and liver levels were 104 µM. LC3B-II protein level was also increased. Form:Solid IC50& Target:IC50: 24.5 µM (ATG4B),Kd: 16 μM (ATG4B)
纯度	≥95%

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

名称和识别符

分子类型	未知
IIUPAC Name	3-[[4-[(E)-2-(7-chloroquinolin-4-yl)ethenyl]phenyl]-(2-phenylethylsulfanyl)methyl]sulfanylpropanoic acid
INCHI	1S/C29H26ClNO2S2/c30-25-12-13-26-23(14-17-31-27(26)20-25)9-6-22-7-10-24(11-8-22)29(35-19-16-28(32)33)34-18-15-21-4-2-1-3-5-21/h1-14,17,20,29H,15-16,18-19H2,(H,32,33)/b9-6+
InChi Key	PHENTWJNXGESKT-RMKNXTFCSA-N
Smiles	C1=CC=C(C=C1)CCSC(C2=CC=C(C=C2)C=CC3=C4C=CC(=CC4=NC=C3)Cl)SCCC(=O)O
Isomeric SMILES	C1=CC=C(C=C1)CCSC(C2=CC=C(C=C2)/C=C/C3=C4C=CC(=CC4=NC=C3)Cl)SCCC(=O)O
PubChem CID	134817239
分子量	520.11

化学和物理性质

溶解性	DMSO : 135 mg/mL (259.56 mM; Need ultrasonic)

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