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C2325742计算溶液所需的质量、体积或浓度。
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基本描述
| 英文别名 | OB gene product | OB Protein | Obesity Protein | OBS | Obesity factor |
|---|---|
| 规格或纯度 | ≥97% (SDS-PAGE) |
| 英文名称 | Leptin (human), (recombinant) |
| 储存温度 | -20°C储存,干燥 |
| 运输条件 | 超低温冰袋运输 |
| 备注 | Lyophilized Leptin protein is stable at room temperature, but should be stored desiccated below 0°C. Reconstituted Leptin is best stored refrigerated at 4°C. |
| 产品介绍 |
Leptin (aka OB protein, or Obesity factor protein) is a 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.|Leptin (human), (recombinant) is produced in E.Coli as a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of approximately 16 kDa. The Leptin protein is purified by proprietary chromatographic techniques.|Biological Activity is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Product description Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant Human Leptin is a 16.0 kDa protein containing 147 amino acid residues. Source E.coli Synonyms Obesity protein (OB) AA Sequence MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC Purity: ≥ 97% by SDS-PAGE gel Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg Accession Number P41159 Gene ID 3952 Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 50 mm PB, pH 3.5, with 0.02 % Tween-20. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. Leptin (aka OB protein, or Obesity factor protein) is a 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.|Leptin (human), (recombinant) is produced in E.Coli as a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of approximately 16 kDa. The Leptin protein is purified by proprietary chromatographic techniques.|Biological Activity is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Product description Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant Human Leptin is a 16.0 kDa protein containing 147 amino acid residues. Source E.coli Synonyms Obesity protein (OB) AA Sequence MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC Purity: ≥ 97% by SDS-PAGE gel Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg Accession Number P41159 Gene ID 3952 Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 50 mm PB, pH 3.5, with 0.02 % Tween-20. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 纯度 | ≥97%(SDS-PAGE) |
化学和物理性质
| 溶解性 | Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point |
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安全和危险性（GHS）
| 预防措施声明 |
P264: 处理后要彻底洗手。 |
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