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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| L304912-1mg |
1mg |
期货 ![]() |
| |
| L304912-5mg |
5mg |
现货 ![]() |
| |
| L304912-25mg |
25mg |
期货 ![]() |
|
| 产品名称 | 重组溶葡萄球菌酶 |
|---|---|
| 别名 | 溶葡萄球菌素 | 溶葡球菌酶 | 甘氨酰-甘氨酸内肽酶 |
| 英文别名 | glycyl-glycine endopeptidase | lss | rLysostaphin,rLSS |
| 规格或纯度 | 重组,EnzymoPure™,≥90%(SDS-PAGE),≥1200 U/mg solid |
| 生化机理 | 通过水解肽聚糖的聚甘氨酸肽间桥来裂解葡萄球菌细胞。催化活性:连接葡萄球菌细胞壁肽聚糖的五甘氨酸肽链中-Gly-|-Gly-键的水解。辅因子:Zn2+。注:每个亚基结合1个锌离子。 |
| 产品介绍 |
溶葡球菌酶(Lysostaphin)是模拟葡萄球菌分泌的一种专门溶解溶葡萄球菌的溶菌酶,是一种含锌的肽链内切酶,裂解葡萄球菌肽聚糖的“甘氨酰五肽”键,使得细菌溶胀裂解死亡。能特异性杀灭金黄色葡萄球菌和表皮葡萄球菌,也包括可裂解造成致命感染的耐甲氧西林金黄色葡萄球菌和耐万古霉素金黄色葡萄球菌的细胞壁。该酶也能裂解其它革兰氏阳性菌细胞壁的甘氨酸肽键,还能够水解富含甘氨酸的蛋白质,如不溶性弹性蛋白。本品是采用基因工程重组表达技术生产的高纯度高活性溶葡球菌酶。可用于日常杀菌、临床检验、科学研究、食品保鲜等领域。 序列相似性:Belongs to the peptidase M23B family. 保存和稳定性 1、该酶冻干粉剂在4℃至少保存两年活性不下降; 2、酶溶液(pH5~8)在4℃放置,10天内基本稳定。 Lysostaphin is a specialized lysozyme that simulates the secretion of Staphylococcus aureus. It is a zinc containing peptide chain endonuclease that cleaves the "glycyl pentapeptide" bond of Staphylococcus peptidoglycan, causing bacterial swelling, lysis, and death. It can specifically kill Staphylococcus aureus and Staphylococcus epidermidis, as well as the cell walls of methicillin-resistant Staphylococcus aureus and vancomycin resistant Staphylococcus aureus that can cause fatal infections by lysis. This enzyme can also cleave glycine peptide bonds in the cell walls of other Gram positive bacteria, and hydrolyze proteins rich in glycine, such as insoluble elastin. This product is a high-purity and highly active lysosomal enzyme produced using genetic engineering recombinant expression technology. Can be used in daily sterilization, clinical testing, scientific research, food preservation and other fields. Sequence similarity: Belongs to the peptidase M23B family Preservation and stability 1. The enzyme freeze-dried powder should be stored at 4 ℃ for at least two years without any decrease in activity; 2. The enzyme solution (pH 5-8) remains stable at 4 ℃ for 10 days. |
| 生物活性 | ≥1200 U/mg solid |
| 表达系统 | E.coli & Pichia pastoris |
| 种属 | |
| Accession # | P10547 |
| 来源 | 重组表达 |
| 预测分子量 | 27 kDa |
| 物理形态 | 冻干(Lyophilized) |
|---|---|
| 浓度 | ≥1200 U/mg solid |
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 长期储存2-8℃或-20℃(2年);密封。 |
| CAS编号和信息 | 9011-93-2 |
| 酶学委员会编号 | 3.4.24.75 |
| 单位定义 | 使每毫升金葡菌菌液37℃,每分钟OD620吸收值降低0.01所需的酶量。 |
| 分子类型 | 酶 |