| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| I755863-500ml |
500ml |
期货  |
|
| 规格或纯度 | 无菌过滤, 用于细胞培养, With stable glutamine and sodium bicarbonate, liquid |
|---|---|
| 英文名称 | IMDM |
| 储存温度 | 2-8°C储存 |
| 运输条件 | 冰袋运输 |
| 产品介绍 |
经 Guilbert 和 Iscove 证明，红细胞和巨噬细胞的前体细胞可在添加白蛋白、转铁蛋白、卵磷脂和硒的完全确定的无血清培养基中培养。该培养基是对 Dulbecco 改良 Eagle 培养基 (DME) 的改良，含有硒、额外的氨基酸和维生素、丙酮酸钠、HEPES 缓冲液和硝酸钾而不是硝酸铁。进一步研究证明，Iscove 培养基可支持小鼠 B 淋巴细胞、骨髓造血组织、脂多糖刺激的 B 细胞、T 淋巴细胞和多种杂交细胞。应用： 该 IMDM 与原处方不同，因为其添加了 L-丙氨酰-L-谷氨酰胺二肽。众所周知，谷氨酰胺在细胞培养中是不稳定的。这种二肽为细胞培养提供了一种更稳定的谷氨酰胺形式。 This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures. Application:Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells. |
首页
400-620-6333
