基本描述

别名

脱盐小柱配套试剂 | 脱盐柱试剂

英文别名

Desalting Column Reagents

规格或纯度

BioReagent, 无菌, 蛋白组学级

英文名称

Peptide Desalting Columns Supporting Reagent

储存温度

避光,室温

运输条件

常规运输

产品介绍

脱盐柱配套试剂可处理酶解后的除盐环节，快速制备前处理样品，用于后续的质谱分析。

操作流程示意图

产品组分及储存条件：

H1456342	Component	25T	48T	96T	Storage
H1456342A	Wash buffer 1	9.5 mL	17.5 mL	35 mL	RT
H1456342B	Wash buffer 2	6 mL	12 mL	24 mL	RT
H1456342C	Wash buffer 3	6 mL	12 mL	24 mL	RT
H1456342D	Elution buffer	12 mL	25 mL	50 mL	RT. Store in the dark.
H1456342E	Loading buffer	500 μL	1 mL	2 mL	RT

产品规格*（若无脱盐小柱，可联系我司购买，货号： P1456352）

操作流程：

①向酶解完成后的样品中加320μL的Wash buffer 1，剧烈震荡3min，15000rpm离心3min，将上层液体去除。

②将下层样品转入Tip柱中，2500rpm/min，离心3-5min，直到液体全部离心下来，如果液体流速较慢，可以加大转速。

③向脱盐柱中加入200μL的Wash buffer 2 (用之前震荡10-20s)，2500rpm/min，离心3-5min，直到液体全部离心下来。

④向脱盐柱中加入200μL的Wash buffer 3，2500rpm/min，离心3-5min，直到液体全部离心下来。

⑤将脱盐柱重新放入一个新的EP管中，向脱盐柱中加入200μL的Elution buffer，2000rpm/min，离心3-5min，直到液体全部离心下来。

⑥重复步骤⑤，收集两次的洗脱液，冷冻干燥。

⑦加入10μL的Loading buffer，剧烈涡匀3min，离心20000g、10min，取适量样品，即可质谱检测。以HF-X仪器为例，上样0.5-1μg即可。

注意事项和免责说明：

本产品仅限于专业，人员的科学研究使用，不得用于临床诊断或治疗，不得用于食品或药品。

Supporting Reagent with the desalting column can handle the desalting step after enzymatic hydrolysis, quickly prepare pretreated samples, and are used for subsequent mass spectrometry analysis.

Schematic diagram of the operation process

Product Components and Storage Conditions:

H1456342	Component	25T	48T	96T	Storage
H1456342A	Wash buffer 1	9.5 mL	17.5 mL	35 mL	RT
H1456342B	Wash buffer 2	6 mL	12 mL	24 mL	RT
H1456342C	Wash buffer 3	6 mL	12 mL	24 mL	RT
H1456342D	Elution buffer	12 mL	25 mL	50 mL	RT. Store in the dark.
H1456342E	Loading buffer	500 μL	1 mL	2 mL	RT

Operating Procedure:

① Add 320μL of Wash buffer 1 to the sample after enzymatic hydrolysis, shake vigorously for 3 minutes, centrifuge at 15000rpm for 3 minutes, and remove the upper liquid.
② Transfer the lower layer sample into the Tip column, centrifuge at 2500rpm for 3-5 minutes until all the liquid is centrifuged down. If the liquid flow rate is slow, the rotation speed can be increased.
③ Add 200μL of Wash buffer 2 (shake for 10-20 seconds before use) to the desalting column, centrifuge at 2500rpm for 3-5 minutes until all the liquid is centrifuged down.
④ Add 200μL of Wash buffer 3 to the desalting column, centrifuge at 2500rpm for 3-5 minutes until all the liquid is centrifuged down.
⑤ Put the desalting column into a new EP tube, add 200μL of Elution buffer to the desalting column, centrifuge at 2000rpm for 3-5 minutes until all the liquid is centrifuged down.
⑥ Repeat step ⑤, collect the eluates from both times, and freeze-dry them.
⑦ Add 10μL of Loading buffer, vortex vigorously for 3 minutes, centrifuge at 20000g for 10 minutes, take an appropriate amount of sample for mass spectrometry detection. Taking the HF-X instrument as an example, 0.5-1μg of sample is sufficient for loading.

Precautions and Disclaimer:
This product is limited to scientific research use by professional personnel. It must not be used for clinical diagnosis or treatment, nor for food or drugs.