Human Fc Receptor Blocking Solution

COA

级别和纯度:

无菌过滤

BioReagent

10 μL/test

有货

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货号 (SKU)	包装规格	是否现货
H1374078-25T	25T	现货
H1374078-100T	100T	现货
H1374078-5×100T	5×100T	现货

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封闭液 (18) 流式细胞术 (1163)

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基本描述

别名	单核/巨噬细胞阻断剂 \| Fc受体封闭剂 \| Fc受体阻断剂 \| 流式细胞封闭剂 \| FcR阻断剂 \| 人Fc受体封闭剂 \| 人单核/巨噬细胞阻断剂 \| 花菁染料封闭液
英文别名	Cyanine dye blocking solution \| Blocking buffer for Flow \| Blocking Reagent \| Monocyte Blocker \| Fc Receptor Blocker \| FcR Blocking Reagent \| Fc Receptor Blocking Solution
规格或纯度	无菌过滤, BioReagent, 10 μL/test
稳定性与储存	Store at 2-8°C for 12 months. When stored at 2°C to 8°C, the presence of serum in this product may cause aggregation of various proteins and lipoproteins (such as cold agglutinins, fibrinogen, vitronectin, etc.), forming visible precipitate or cloudiness.
英文名称	Human Fc Receptor Blocking Solution
储存温度	2-8°C储存,避光
运输条件	冰袋运输
产品介绍	储存缓冲液：0.05% ProClin 300 是否无菌：是 Non-specific monocyte binding remains a ubiquitous challenge in flow cytometry. Although the exact mechanism is undefined, literature implicates CD64 interactions with cyanine dyes. However, our data demonstrate that non-specific binding extends beyond cyanine-based fluorophores: certain non-cyanine fluorescent dyes also exhibit this phenomenon. Crucially, we observed selective non-specific staining on monocytes for all tested PE and APC tandem dyes. Human Fc Receptor Blocking Solution is a non-antibody blocking solution and has been formulated to block non-specific binding of PE-Cy7, PE-Cy5, PE-Cy5.5, PE-APC, APC-Cy7, and AF647 which is also a cyanine dye commonly appeared on monocytes and macrophages. This buffer contains specialized human IgG containing 0.05% ProClin 300. It is not recommended to be used for staining human IgG. Handle as biohazard agent. Human peripheral blood leukocytes were either not preincubated (Upper Plots) or were preincubated (Lower Plots) with Human Fc Receptor Blocking Solution as indicated. The cells were then selectively stained with either Mouse IgG1-kappa (PE-Cy5.5), anti-E8L (Ab216584), Mouse IgG1-kappa (PE-Cy7), anti-E8L (Ab216583), Mouse IgG2b-kappa (PE-Cy5), anti-E8L (Ab216565), Mouse IgG2c-kappa (PE-Cy5), anti-HEL (Ab212231) or Mouse IgG2c-kappa (PE-Cy7), anti-HEL (Ab212232). Two-dimensional pseudocolor plots of SSC-A vs Mouse IgG show that the Human Fc Receptor Blocking Solution reduces non-specific binding compared to staining without any blocking reagent. Human peripheral blood leukocytes were either not preincubated any blocking reagent (Top Plots) or were preincubated with competitor Fc Receptor Blocking reagent (Middle Plots) or were preincubated with Human Fc Receptor Blocking Solution (Bottom Plots) as indicated. The cells were then selectively stained with either Mouse IgG (APC-Cy7) (Ab213860), Mouse IgG2b-kappa (PE-Cy5), anti-E8L (Ab216565) or Mouse IgG2c-kappa (PE-Cy5), anti-HEL (Ab212231). Two-dimensional pseudocolor plots of SSC-A vs Mouse IgG show that the Human Fc Receptor Blocking Solution reduces non-specific binding compared to staining without any blocking reagent or with competitor Fc Receptor Blocking reagent.

图片

Flow Cytometry: Human Fc Receptor Blocking Solution (H1374078)

Human Fc Receptor Blocking Solution (H1374078) - Flow Cytometry
Human peripheral blood mononuclear cells were either untreated (black) or treated with Human Fc Receptor Blocking Solution (H1374078) (red) and stained with recombinant CD14 Antibody (AF405) (Ab214465) and CD3 Mouse mAb (PE-Cy5) (Ab189665) (left), CD3 Mouse mAb (PE-Cy7) (Ab189666) (middle), or CD3 Mouse mAb (AF647) (Ab189663).

Flow Cytometry: Human Fc Receptor Blocking Solution (H1374078)

Human Fc Receptor Blocking Solution (H1374078) - Flow Cytometry
Flow Cytometry analysis of no Human Fc Receptor Blocking Solution (left) or with Human Fc Receptor Blocking Solution (H1374078) (right) blocked human peripheral blood monocyte labelling CD11b (red) with CD11b Mouse mAb (PE-Cy5) (Ab191617) at 1.1 μg/mL for 1 hour at 4°C. Blue - Mouse IgG (PE-Cy5) (Ab190078). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Human Fc Receptor Blocking Solution (H1374078)

Human Fc Receptor Blocking Solution (H1374078) - Flow Cytometry
Human peripheral blood leukocytes were either not preincubated (Left Plots) or were preincubated (Right Plots) with Human Fc Receptor Blocking Solution (H1374078) as indicated. The cells were then stained with Mouse IgG (PE-Cy5) (Ab190078). Two-dimensional pseudocolor plots of SSC-A vs Mouse IgG show that the Human Fc Receptor Blocking Solution reduces non-specific binding compared to staining without any blocking reagent.

应用

应用名称	稀释比例
Flow Cytometry	10 μL/test. It is recommended to add 10 µL of Human Fc Receptor Blocking Solution per million cells in 100 µl staining volume, mix and incubate at 4 °C for 30 minutes prior to staining with antibody of inter

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批号(Lot Number)	证书类型	货号
ZJ25F0825781	分析证书	H1374078
ZJ25F0825782	分析证书	H1374078

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Human Fc Receptor Blocking Solution

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