计算溶液所需的质量、体积或浓度。
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| 别名 | 6X DNA上样缓冲液 | 6X DNA上样染料 | 6X DNA上样液 | 琼脂糖凝胶上样缓冲液 | 琼脂糖凝胶上样染料 | 琼脂糖凝胶上样液 | 样品上样缓冲液 | 样品上样染料,样品上样液 |
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| 英文别名 | 6X DNA loading buffer | 6X DNA loading dye | 6X DNA loading solution | agarose gel loading buffer | agarose gel loading dye | agarose gel loading solution | sample loading buffer | sample loading dye | sample loading solution |
| 规格或纯度 | 用于核酸电泳 |
| 英文名称 | Gel Loading Buffer |
| 生化机理 | 凝胶上样缓冲液特别配制用于非变性聚丙烯酰胺和核酸的琼脂糖凝胶电泳。凝胶上样溶液含有溴酚蓝跟踪染料和蔗糖,以增加密度,并促进样品上样。已包括 EDTA,以抑制需要二价阳离子的核酸酶,并且已添加 SDS,以帮助解离可干扰电泳的 DNA-蛋白质复合物。 |
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 备注 | 如果形成晶体,可以将溶液加热至 65℃,10 分钟将其溶解。 |
| 产品介绍 |
凝胶上样缓冲液用作电泳过程中的跟踪染料。 染料具有轻微的负电荷,并且将与 DNA 相同的方向迁移,允许用户监测分子在凝胶中移动的进程。 迁移速率随凝胶组成而变化。 上样前将样品以 1:3 至 1:6 进行稀释。 应用 凝胶上样缓冲液用于上样聚合酶链反应(PCR)扩增的基因间隔区(ISR)序列样本 PCR扩增的肠粘膜DNA样本锥虫属NDA在琼脂糖凝胶上进行电泳。适用于琼脂糖或非变性聚丙烯酰胺凝胶电泳(PAGE),可用作Northern印迹和Southern印迹杂交程序的一部分。 组分
凝胶上样缓冲液含有 色素溴酚蓝、二甲苯腈蓝 FF(Xylene Cyanol FF)和甘油等成分;
Gel loading buffer is used as a tracking dye during electrophoresis. The dye has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample before loading.Gel Loading Buffer has been used for loading polymerase chain reaction (PCR) amplified intergenic spacer region (ISR) sequence samples, PCR amplified DNA samples of the intestinal mucosa,Trypanosoma spDNA on agarose gel for electrophoresis.It is suitable for use with agarose or non-denaturing polyacrylamide gel electrophoresis (PAGE), which may be part of Northern and Southern blot hybridization procedures. |
| 溶解性 | Soluble in water at 20 °C |
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