This is a demo store. No orders will be fulfilled.

凝胶加载溶液

有货

库存信息

关闭

库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
G485241-100ml
100ml 期货 Stock Image
G485241-500ml
500ml 期货 Stock Image

基本描述

规格或纯度 适用于NA电泳,溶液
英文名称 Gel Loading Solution
储存温度 2-8°C储存
运输条件 冰袋运输
备注 预期条带迁移如下:在聚丙烯酰胺凝胶上,二甲苯氰基可与大约450-460bp的DNA共迁移,而溴酚蓝与15-100bp的DNA共迁移。在0.5-1.4%琼脂糖凝胶上,二甲苯氰基可与4kb dsDNA共迁移,而溴酚蓝则与300bp dsDNA共迁移。
产品介绍

凝胶上样溶液在电泳期间可用作跟踪染料。染料具有轻微的负电荷,并且与DNA的迁移方向相同,从而使得用户可以监测分子在凝胶中移动的进程。迁移速率随凝胶组成而变化。上样前用样品进行1:6稀释。


应用

适用于琼脂糖或非变性聚丙烯酰胺凝胶电泳(PAGE),该电泳步骤可能是Northern和Southern印迹杂交过程的一部分。


适用性



原理



制备说明



外形



重悬



组分

凝胶上样缓冲液含有0.25%溴酚蓝、0.25%二甲苯蓝和40%蔗糖。

Gel loading solution is used as a tracking dye during electrophoresis. The dyes have a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:6 with sample before loading.Suitable for use with agarose or non-denaturing polyacrylamide gel electrophoresis (PAGE), which may be part of Northern and Southern blot hybridization procedures.

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

引用文献

1. David R Macinga,Paul J Renick,Kelly M Makin,David H Ellis,Allison A Kreiner,Min Li,Kirk J Rupnik,Erica M Kincaid,Cynthia D Wallace,Benoit Ledoussal,Timothy W Morris.  (2003-07-25)  Unique biological properties and molecular mechanism of 5,6-bridged quinolones..  Antimicrobial agents and chemotherapy,  47  ((8)): (2526-2537).  [PMID:12878515]
2. Jung-Lim Lee,Robert E Levin.  (2006-12-22)  Use of ethidium bromide monoazide for quantification of viable and dead mixed bacterial flora from fish fillets by polymerase chain reaction..  Journal of microbiological methods,  67  ((3)): (456-462).  [PMID:17183624]
3. Peter Konings,Evelyne Vanneste,Sigrun Jackmaert,Michèle Ampe,Geert Verbeke,Yves Moreau,Joris Robert Vermeesch,Thierry Voet.  (2012-01-21)  Microarray analysis of copy number variation in single cells..  Nature protocols,  ((2)): (281-310).  [PMID:22262009]
4. S Henry,R Mollicone,J B Lowe,B Samuelsson,G Larson.  (1996-01-01)  A second nonsecretor allele of the blood group alpha(1,2)fucosyl-transferase gene (FUT2)..  Vox sanguinis,  70  ((1)): (21-25).  [PMID:8928486]
5. M K Bolla,N Wood,S E Humphries.  (1999-12-10)  Rapid determination of apolipoprotein E genotype using a heteroduplex generator..  Journal of lipid research,  40  ((12)): (2340-2345).  [PMID:10588960]

溶液计算器