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甘油3-磷酸氧化酶

1篇引用文献

COA

级别和纯度:

EnzymoPure™

≥35U/mg

有货

库存信息

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货号 (SKU)	包装规格	是否现货
G196970-0.5KU	0.5KU	现货
G196970-1KU	1KU	现货
G196970-5KU	5KU	现货
G196970-10KU	10KU	现货

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代谢物 (8911) 磷酸氧化酶 (1) 酶类 (34)

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基本描述

产品名称	甘油3-磷酸氧化酶
别名	甘油3-磷酸氧化酶 \| sn-3-磷酸甘油:2-氧氧化还原酶 \| 3-磷酸甘油氧化 \| 酶
英文别名	sn-Glycerol 3-phosphate:oxygen 2-oxidoreductase \| L-Glycerol 3-phosphate Oxidase
规格或纯度	EnzymoPure™, ≥35U/mg
产品介绍	活力定义： One unit is defined as the enzyme quantity which oxidizes one μ mole of L-a-Glycerophosphate per minute under the condition described below. Reagents A. Aminoantipyrine Solution (5 mg/mi) Weigh 0.25 g of s-aminoantipyrinc and dissoive in 20 ml ol dcionized water. Fill up to 50ml with deionized water. B. Phenol Solution (50 mg/ ml) Weigh 5.0 g of phenol and dissolve in 30 ml of deionized water. Fill up to 100 ml with deionized water. C. 0.2M Phosphate Buffer (KH2PO4-NaOH, pH 20) D. 002M Phosphate Buffer (KH2PO4-NaOH, pH 20) E. Aminoantipyrine-Phenol Solution Dilute 6 ml of Aminoantipyrine Solution (A), 0.5 ml of Phenol Solution (B) and 200 purpurogallin units of Per-oxidase (produced by Amano Enzyme Inc.) with 0.2M Phosphate Buffer (C). Fill up to 50 ml with 0.2M Phosphate Buffer (C). (Expires after 14 days at 2-8°C) F. Substrate Solution Weigh 36.7 g of DL-α-glycerophosphate·2Na·5H2O (produced by Wako Pure Chemical Industries, Ltd. ) and dissolve in approx. 40 ml of deionized water. Adjust the pH to 7.0 with 4N HCl and fill up to 100 ml with deionized water. Then, filtrate the solution with 0.45 um membrane filter. G. Diluent Weigh 1.00 g of bovine serum albumin (produced by Wako Pure Chemical Industries, Ltd.: Fraction V), 66.0 g of (NH,),SO, and dissolve in 002M Phosphate Buffer (D). Fil up to 1,000 ml with 0.02M Phosphate Buffer (D). (Expires after 1 month at 2-8°C) H. Enzyme Solution Weigh 20 mg of L-a-Glycerophosphate Oxidase "Amano" and dissolve in chilled Diluent (G). Enzyme Solution should be prepared so that the value of △OD/ minute becomes in the range of 0.020±0.005 甘油三酯的氧化用于医学检测甘油三酯 This enzyme is used for the enzymatic determination of triglyceride in serum by coupling with lipoprotein lipase and glycerol kinase in clinical diagnosis.

储存与运输

浓度	≥35U/mg
储存温度	-20°C储存
运输条件	超低温冰袋运输
CAS编号和信息	9046-28-0
酶学委员会编号	EC 1.1.3.21

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找到9个结果

批号(Lot Number)	证书类型	货号
F2413115	分析证书	G196970
F2413117	分析证书	G196970
F2413121	分析证书	G196970
F2413119	分析证书	G196970
F2413116	分析证书	G196970
F2413118	分析证书	G196970
F2413120	分析证书	G196970
F2413122	分析证书	G196970
F2412119	分析证书	G196970

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引用文献

1. Jia Xu, Habib Khan, Li Yang. (2021) Hydrogel Paper-Based Analytical Devices: Separation-Free In Situ Assay of Small-Molecule Targets in Whole Blood. ANALYTICAL CHEMISTRY, 93 (44): (14755–14763). [PMID:34709797] [10.1021/acs.analchem.1c03347]