按主题分类的文章 "Buffer"

1. Antibody Purification Tag Strategies and Applications
   发布于 2025年7月28日

   9
   Antibodies, as core molecules in biopharmaceuticals, play a crucial role in determining both product quality and production costs. Tag technologies, as key tools in antibody purification, have evolved from mature applications in classical systems to innovative breakthroughs in emerging systems, ...
2. PCR Laboratory Contamination Prevention and Efficient Reagent Application: —Optimizing Experimental Steps and Choosing
   发布于 2025年2月24日

   2
   Polymerase Chain Reaction (PCR) is a core technology in molecular biology, clinical diagnostics, and scientific research. Its high sensitivity means that even minute contaminants can affect experimental results, leading to false positives or false negatives.
3. Can tumor tissue cuts be stored for a long time after fixation in 4% paraformaldehyde fixative at room temperature?
   发布于 2024年8月28日

   1
   Can tumor tissue cuts be stored for a long time after fixation in 4% paraformaldehyde fixative at room temperature?
4. Protein samples boiled in buffer are blue in color and turn blue-violet after being left at -80°C. What is the reason for this? Does it affect the subsequent electrophoresis?
   发布于 2024年8月28日

   1
   Protein samples boiled in buffer are blue in color and turn blue-violet after being left at -80°C. What is the reason for this? Does it affect the subsequent electrophoresis?
5. BCA concentration measured protein concentration 3mg/ml, but after adding buffer found no protein bands appeared, what is the reason?
   发布于 2024年8月28日

   1
   BCA concentration measured protein concentration 3mg/ml, but after adding buffer found no protein bands appeared, what is the reason?
6. Is there a requirement for protein concentration for this product? If the protein concentration is high, do I need to increase the concentration of the buffer?
   发布于 2024年8月28日

   1
   Is there a requirement for protein concentration for this product? If the protein concentration is high, do I need to increase the concentration of the buffer?
7. What is the reason why the protein after denaturation by adding buffer tends to be difficult to sink and float up easily when spotting? What should I do to solve this problem?
   发布于 2024年8月28日

   0
   What is the reason why the protein after denaturation by adding buffer tends to be difficult to sink and float up easily when spotting? What should I do to solve this problem?
8. After adding buffer to boil denatured proteins for cryopreservation, do they still need to be boiled after they are removed again? Why?
   发布于 2024年8月28日

   0
   After adding buffer to boil denatured proteins for cryopreservation, do they still need to be boiled after they are removed again? Why?
9. What is the reason that the protein added to the buffer cooks without precipitation, but the next day when the sample is removed and spiked, precipitation appears? What should be done?
   发布于 2024年8月27日

   1
   What is the reason that the protein added to the buffer cooks without precipitation, but the next day when the sample is removed and spiked, precipitation appears? What should be done?
10. If the protein contains polymer heterobands, dimers may be present, do I need to add doubling buffer to open the dimers?
    发布于 2024年8月27日

    0
    If the protein contains polymer heterobands, dimers may be present, do I need to add doubling buffer to open the dimers?