按主题分类的文章 "Protein Biology"

1. Boc resin lysis protocol
   发布于 2024年8月22日

   9
   In peptide synthesis, Boc protection groups are widely used to protect the amino terminus of amino acids to prevent unnecessary reactions during synthesis. After the peptide synthesis is complete, the peptide is released from the resin through the cleavage and deprotection steps, and these ...
2. Solid-Phase Peptide Synthesis (SPPS) — Efficient Strategies and Innovative Techniques
   发布于 2024年8月21日

   2
   Solid-phase peptide synthesis (SPPS) is a revolutionary biochemical technique that has become the primary method for synthesizing peptides and proteins since its introduction in the 1960s. Robert Bruce Merrifield first proposed the concept of SPPS in 1963, and this groundbreaking work not only ...
3. Method and Precautions of SDS-PAGE Gel Preparation
   发布于 2024年5月20日

   0
   SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a commonly used technique to separate proteins based on size. By denaturating the protein and giving it a uniform negative charge, the protein migrates to the positive pole under the application of an electric field and is ...