基本描述

英文别名	FICA
英文名称	Freund's Incomplete Adjuvant
储存温度	2-8°C储存,避光
运输条件	冰袋运输
产品介绍	仅用于抗体制备和动物免疫学相关研究，不得用于人类或当药品使用。应用： 1、弗氏佐剂可用于产生免疫原的油包水乳剂。油包水乳剂中的抗原可刺激高且持久的抗体应答，而这可归因于抗原的缓慢释放。 2、弗氏（不完全）佐剂缺乏在弗氏完全佐剂中发现的分枝杆菌，因此它可以最大限度地减少副作用。它用于增强注射。它用于乳化注射到动物体内的抗原，从而抗血清中产生单克隆抗体。说明： 1.免疫：本产品与抗原混合搅拌能很快形成油包水状态，在1:1-1:2情况下都能形成稳定的乳液，1:2乳化较1:1粘稠些，乳化后滴水数周不散；经多品种抗原多种属动物测试免疫效果极好，由于乳化彻底牢固，因此跟其他佐剂相比比较不容易出现免疫耐受现象。 2.单抗腹水制备：将佐剂注射BALB/C小鼠腹腔，每只0.3-0.5ml处理腹腔15天左右（一般12-18天），然后注射杂交瘤细胞，建议每只注射80万-100万个。腹水会比普通佐剂晚出1.5天-2天，但通常情况下腹水的量是普通佐剂出量的1.5-2倍，且单抗腹水效价不变。 Only used for antibody preparation and animal immunology related research, not for human or drug use. Application： 1. Freund's adjuvant can be used as an oil in water emulsion for producing immunogens. The antigens in oil-in-water emulsions can stimulate high and persistent antibody responses, which can be attributed to the slow release of antigens. 2. Freund's (incomplete) adjuvant lacks the mycobacteria found in Freund's complete adjuvant, so it can minimize side effects. It is used to enhance injection. It is used to emulsify antigens injected into animal bodies, thereby producing monoclonal antibodies in the antiserum. Explanation： 1. Immunity: This product can quickly form a water in oil state when mixed with antigen. Stable lotion can be formed under the conditions of 1:1-1:2. 1:2 emulsification is thicker than 1:1 emulsification. After emulsification, water will not disperse for several weeks; The immune effect is excellent after testing multiple types of antigens and animal genera, due to the milk Thoroughly and firmly solidified, it is less likely to exhibit immune tolerance compared to other adjuvants. 2. Preparation of monoclonal antibody ascites: Adjuvants are injected into the abdominal cavity of BALB/C mice, with each mouse treated with 0.3-0.5ml for about 15 days (usually 12-18 days), and then hybridoma cells are injected. It is recommended to inject 800000-100000 cells per mouse. Ascites may be delayed by 1.5-2 days compared to regular adjuvants, but there is no significant difference Under normal circumstances, the amount of ascites is 1.5-2 times that of a regular adjuvant, and the efficacy of monoclonal antibodies in ascites remains unchanged.

名称和识别符

分子类型	未知

化学和物理性质

敏感性	对光敏感

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批号(Lot Number)	证书类型	货号
D2522313	分析证书	F393371
D2522314	分析证书	F393371
J2411819	分析证书	F393371
J2411868	分析证书	F393371
J2411869	分析证书	F393371
F2414287	分析证书	F393371
F2414288	分析证书	F393371
D2407283	分析证书	F393371
G2307800	分析证书	F393371
A2312054	分析证书	F393371

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引用文献

1. Jia-Hui Ma, Li-Qiu Ren, Tian-Xuan Tang, Yu-Yao Chen, Chen-Xi Zhang, Yu-Feng Ke, Yan Zhang, Mitchell N. Muskat, Xiang-Rong Cheng. (2024) Effects of polyphenols from walnut pellicle on the structure and allergenicity of walnut globulin. Food Bioscience, 60 (104381). [10.1016/j.fbio.2024.104381]
2. Xiaofang Li, Jinyan Li, Yongwei Feng, Liqiang Liu, Hua Kuang, Chuanlai Xu, Lingling Guo. (2024) Fluorescent microsphere immunochromatographic sensor for the detection of total fumonisins B1, B2, and B3 in grain samples. JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 128 (106018). [10.1016/j.jfca.2024.106018]
3. Qianqian Lu, Liqiang Liu, Jinyan Li, Shanshan Song, Hua Kuang, Chuanlai Xu, Lingling Guo. (2024) Rapid and sensitive quantitation of amitraz in orange, tomato, and eggplant samples using immunochromatographic assay. FOOD CHEMISTRY, 446 (138899). [PMID:38452506] [10.1016/j.foodchem.2024.138899]
4. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu. (2024) Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method. Food Bioscience, 61 (104936). [10.1016/j.fbio.2024.104936]

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货号 (SKU)	包装规格	是否现货	价格	数量
F393371-10ml	10ml	现货
F393371-25ml	25ml	现货

弗氏不完全佐剂

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