| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| E666128-4KU |
4KU |
期货  |
| |
| E666128-20KU |
20KU |
期货 |
|
| 别名 | 外切酶I(Exo I) | ||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 英文名称 | Exonuclease I（Exo I） | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| 储存温度 | -20°C储存,避免反复冻融 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| 运输条件 | 超低温冰袋运输 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| 产品介绍 |
产品内容
产品简介
本产品来源于重组E.coli菌株，其携带有Exo I基因，具有从3’-5’方向水解单链DNA的
外切酶活性，能够逐步释放脱氧核糖核酸5’单磷酸，并留下完整的5’端二核苷酸。本产品
主要应用于PCR扩增后降解消化引物，对于双链DNA和磷酰或乙酰基团封闭的3’ OH末端
DNA链无活性。
活性定义
将在37℃条件下，30分钟内催化释放10 nmol的可溶性核苷酸所需的酶量定义为1个
活性单位（U）。
质量控制
经SDS-PAGE电泳检测，经考马斯亮蓝染色后分析其纯度大于95%。加入BSA可
保证酶的稳定性。
使用方法 以下举例PCR产物测序前的清理。该反应去除了单链的引物，降解未配对的核苷酸。 1.将PCR产物与Exonuclease I按下表混合。
2．
混匀后37℃孵育30分钟。
3． 80℃温育20分钟即可失活。 Products Content:
Products Introduction
This product is derived from a recombinant E.coli strain that carries the Exo I gene, which has an exonuclease activity that hydrolyzes single-stranded DNA in the 3'-5' direction, and is capable of gradually releasing the deoxyribonucleic acid 5' monophosphate, leaving the 5' end of the di-nucleotide intact. The product is mainly used for PCR amplification. It is mainly used to degrade digested primers after PCR amplification, and is inactive on double-stranded DNA and 3' OH-terminal DNA strands enclosed by phosphoryl or acetyl groups.
Active Definition The amount of enzyme required to catalyze the release of 10 nmol of soluble nucleotide in 30 minutes at 37°C is defined as 1 unit of activity (U).
quality control The purity of the enzyme is more than 95% after SDS-PAGE electrophoresis and analyzed by Caulmers Brilliant Blue staining. The addition of BSA can ensure the stability of the enzyme.
Usage The following is an example of PCR product cleanup prior to sequencing. This reaction removes single-stranded primers and degrades unpaired nucleotides.
1. Mix the PCR product with Exonuclease I according to the table below.
2. Mix and incubate at 37°C for 30 minutes. 3. Inactivate by incubation at 80°C for 20 minutes. |
首页
400-620-6333
