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StableCell<sup>™</sup> DMEM - 高葡萄糖

    级别和纯度:
  • 无菌过滤
  • 用于细胞培养
  • With 4500 mg/L glucose, stable glutamine, and sodium bicarbonate, without sodium pyruvate., liquid
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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
D755880-500ml
500ml 期货 Stock Image

基本描述

规格或纯度 无菌过滤, 用于细胞培养, With 4500 mg/L glucose, stable glutamine, and sodium bicarbonate, without sodium pyruvate., liquid
英文名称 DMEM - high glucose
储存温度 2-8°C储存
运输条件 冰袋运输
产品介绍

Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。应用:

该 DMEM-Hi 葡萄糖培养基与原始配方不同,因为它补充有L-丙氨酰-L-谷氨酰胺二肽。 众所周知,谷氨酰胺在细胞培养中不稳定。该二肽提供更稳定形式的谷氨酰胺用于细胞培养。它也不同于原始的DMEM-Hi 配方,其中吡哆醇代替吡哆醛。 吡哆醛是一种不稳定的培养基成分。

This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Application:
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name 'DMEM/High'.

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此产品的引用文献

引用文献

1. Wei Cui, Yong Xie, Yinghui Zhang, Xinlian Su, Tianqi Cui, Xingguang Chen, Zhaoming Wang, Feiran Xu, Hui Zhou, Baocai Xu.  (2025)  Antioxidant potential of peptides from poultry hemoglobin via probiotic-assisted hydrolysis: Deciphering mechanisms at the cellular level and through molecular dynamics simulations.  FOOD RESEARCH INTERNATIONAL,  204  (115953).  [PMID:39986793] [10.1016/j.foodres.2025.115953]

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