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Denhardt溶液 50x

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
D485245-50ml
50ml 现货 Stock Image
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生物缓冲液 (83)

基本描述

别名 AA0230
英文别名 blocking reagent | blocking solution | denhardts | hybridization buffer | hybridization solution | northern blocking solution | pre-hybridization | southern blocking solution
规格或纯度 for Northern and Southern blotting, solution
英文名称 Denhardt′s Solution 50x
储存温度 -20°C储存,充氩
运输条件 超低温冰袋运输
产品介绍

50×Denhardt's溶液主要由saccharosan、PVP、BSA 组成,经过无菌处理,主要用于RNA杂交、单拷贝序列的DNA杂交、DNA固定在尼龙膜上的杂交

组分

Denhardt溶液是1%牛血清白蛋白(BSA)、1%saccharosan和1%聚乙烯吡咯烷酮(PVP)的水溶液。


使⽤说明

按实验具体要求操作,或稀释于杂交缓冲液(通常是含有SDS、变性鲑鱼精DNA的SSC)中。


注意事项

1. 如果每次的使用量很小,可以适当分装后再使用,避免反复冻融。

2. 注意无菌操作,防止染菌。

3. 为了您的安全和健康,请穿实验服并戴手套操作。



Description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.Component:Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % saccharosan, and 1 % Polyvinylpyrrolidone (PVP) in water.


名称和识别符

分子类型 未知

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到8个结果

批号(Lot Number) 证书类型 货号
G2529267 分析证书 D485245
E2513204 分析证书 D485245
C2517213 分析证书 D485245
L2417044 分析证书 D485245
C2419122 分析证书 D485245
C2419123 分析证书 D485245
F23011009 分析证书 D485245
F23011010 分析证书 D485245

引用文献

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溶液计算器