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4',6-二脒基-2-苯基吲哚二盐酸盐

荧光DNA染色
134篇引用文献 COA COA
    级别和纯度:
  • ≥98%
有货

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货号 (SKU) 包装规格 是否现货 价格 数量
D106471-5mg
 5mg 现货 Stock Image
D106471-25mg
 25mg 现货 Stock Image
D106471-100mg
 100mg 现货 Stock Image
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Cell Cycle/DNA Damage (2603) DNA Damage (3514) DNA Stain (54) 五元杂环化合物 (2065) 吡咯类 (536)
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基本描述

别名 2-(4-氨基甲酰基苯基)-1H-吲哚-6-羧酰胺二盐酸盐 | 6-脒基-2-(4-脒基苯基)吲哚二盐酸盐 | DAPI 二盐酸盐
英文别名 DAPI (dihydrochloride) | DAPI dihydrochloride | 2-(4-carbamimidoylphenyl)-1h-indole-6-carboximidamide dihydrochloride | 76BFW26YJO | MFCD00012681 | 4',6-Diamidino-2-phenylindole dihydrochloride | DAPI
规格或纯度 ≥98%
英文名称 4',6-Diamidino-2-phenylindole Dihydrochloride
生化机理 荧光DNA染色。与富含AT的DNA区域结合。高浓度时可渗透细胞。
储存温度 -20°C储存
运输条件 超低温冰袋运输
产品介绍

DAPI的最大激发波长为340nm，最大发射波长为488nm；DAPI和双链DNA结合后最大激发波长为364nm，最大发射波长为454nm。DAPI染色溶液(DAPI Staining Solution)是适用于所有常见细胞和组织细胞核染色的染色液。 可以直接用于固定细胞或组织的细胞核染色。 1、DAPI是一种可以穿透细胞膜的蓝色荧光染料。和双链DNA结合后可以产生比DAPI自身强20多倍的荧光。和EB(ethidium bromide)相比，对双链DNA的染色灵敏度要高很多倍。 2、DAPI染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常3、用于普通的细胞核染色以及某些特定情况下的双链DNA染色。

应用

4′,6-二脒基-2-苯基吲哚二盐酸盐应用于免疫细胞化学和细胞化学中各种细胞的染色，人类皮下脂肪组织的荧光成像、免疫荧光胚胎切片。 在琼脂糖凝胶中染色DNA，DAPI比溴化乙锭敏感几倍。它可用于DNA的光足迹图谱，通过对双链复合体的特殊可视化检测印迹应用中的退火探针，并使用流式细胞分析术研究DNA中的变化并分析凋亡过程中的DNA含量。DAPI染色也是一种敏感和特异的支原体检测方法。

DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.
A cell-permeable DNA-binding dye preferential to adenine and thymine rich DNA
纯度 ≥98%

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

名称和识别符

PubChem SID 488188313
EC号 249-186-7
分子类型 小分子
IIUPAC Name 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide;dihydrochloride
INCHI 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChi Key FPNZBYLXNYPRLR-UHFFFAOYSA-N
Smiles C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl
Isomeric SMILES C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl
PubChem CID 160166
分子量 350.25
Beilstein号 4894417
Reaxy-Rn 4894417

化学和物理性质

溶解性 溶于DMSO, 最高浓度 (mg/mL): 35.02, 最高浓度(mM): 100；溶于water, 最高浓度 (mg/mL): 3.5, 最高浓度(mM): 10 温和加热
密度 1.41
敏感性 对光及热敏感
分子量 350.200 g/mol
XLogP3
氢键供体数Hydrogen Bond Donor Count 7
氢键受体数Hydrogen Bond Acceptor Count 2
可旋转键计数Rotatable Bond Count 3
精确质量Exact Mass 349.086 Da
单同位素质量Monoisotopic Mass 349.086 Da
拓扑极表面积Topological Polar Surface Area 116.000 Ų
重原子数Heavy Atom Count 23
形式电荷Formal Charge 0
复杂度Complexity 413.000
同位素原子数Isotope Atom Count 0
定义的原子立体中心计数Defined Atom Stereocenter Count 0
未定义的原子立体中心计数Undefined Atom Stereocenter Count 0
定义的键立体中心计数Defined Bond Stereocenter Count 0
未定义的键立体中心计数Undefined Bond Stereocenter Count 0
所有立体化学键的总数The total count of all stereochemical bonds 0
共价键合单元计数Covalently-Bonded Unit Count 3

安全和危险性（GHS）

象形图 GHS07
信号词 Warning
WGK Germany 3
个人防护装备 Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter

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  2. 4',6-二脒基-2-苯基吲哚二盐酸盐
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