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基本描述
| 产品名称 | SK-N-SH Whole Cell Lysate |
|---|---|
| 别名 | SK-N-SH全细胞裂解物 | SK-N-SH 全细胞裂解物 |
| 规格或纯度 | Mycoplasma free |
| 产品介绍 |
This cell line is used in studies of apoptosis and neuroprotection as an in vitro model of human neuronal cells.
|
储存与运输
| 物理形态 | 冻干(Lyophilized) |
|---|---|
| 储存缓冲液 | Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na₃VO₄). |
| 防腐剂 | No |
| 复溶 | Recommended reconstitute at 1-2 mg/mL in ddH₂O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. |
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 用干冰运输。冻干粉建议在 -80℃ 下保存。复溶后，建议等分，避免反复冻融，短期保存在 -20℃ 下，长期保存在 -80℃ 下。 |
| 分子类型 | 细胞或组织提取物 |
图片
SK-N-SH Whole Cell Lysate (cl185705) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/2000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: HeLa Whole Cell Lysate (cl155894)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: SK-N-SH Whole Cell Lysate (cl185705)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 21.1 s
SK-N-SH Whole Cell Lysate (cl185705) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: HeLa Whole Cell Lysate (cl155894)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: SK-N-SH Whole Cell Lysate (cl185705)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 31 kDa
Exposure time: 113.4 s
SK-N-SH Whole Cell Lysate (cl185705) - Western Blot
All lanes: COX IV Mouse mAb (Ab097414) at 1/2000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: HeLa Whole Cell Lysate (cl155894)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: SK-N-SH Whole Cell Lysate (cl185705)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa
Exposure time: 22.9 s
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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